FlyBase Allele Report: Dmel\Atg13[UAS.cCa]

General Information

Symbol

Dmel\Atg13^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chang

FlyBase ID

FBal0221503

Feature type

allele

Associated gene

Dmel\Atg13

Associated Insertion(s)

Carried in Construct

P{UAS-Atg13.C}

Also Known As

UAS-Atg13

Key Links

Transgenic product class

cDNA

(Chang and Neufeld, 2009)

wild_type

(Chang and Neufeld, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Chang and Neufeld, 2009)

wild_type

(Chang and Neufeld, 2009)

Mutagen

in vitro construct

(Chang and Neufeld, 2009)

Progenitor genotype

Carried in construct

P{UAS-Atg13.C}

Cytology

Description

UASt regulatory sequences drive expression of Atg13 cDNA LD09558.

(Chang and Neufeld, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atg13

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Niemann-Pick disease

modeled by Npc1a^KK101077

(Danielsen et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

The amelioration of the phenotype in Npc1a^KK101077 expressing flies is observed upon simultaneous co-expression of both Atg1^Scer\UAS.cSa and Atg13^Scer\UAS.cCa. sf161013

(Danielsen et al., 2016)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Atg13^Scer\UAS.cCa under the control of Scer\GAL4^Cg.PA does not induce formation of autophagic vesicles (as detected by Atg8a signal).

(Braden and Neufeld, 2016)

Expression of Atg13^Scer\UAS.cCa under the control of Scer\GAL4^Act5C.PP in fat body cell clones has no appreciable effect on cell size.

Expression of Atg13^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF has no discernible effect on eye development.

Expression of Atg13^Scer\UAS.cCa under the control of Scer\GAL4^Cg.PA does not affect larval growth or developmental timing.

(Chang and Neufeld, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^P0206, Atg1^UAS.cSa/Atg13^UAS.cCa is a suppressor of abnormal developmental rate phenotype of Npc1a^KK101077, Scer\GAL4^P0206

(Danielsen et al., 2016)

Scer\GAL4^P0206, Atg1^UAS.cSa/Atg13^UAS.cCa is a suppressor of increased body size | pupal stage phenotype of Npc1a^KK101077, Scer\GAL4^P0206

(Danielsen et al., 2016)

Other

Statement

Reference

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP has decreased cell size phenotype

(Chang and Neufeld, 2009)

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype

(Chang and Neufeld, 2009)

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has lethal | first instar larval stage phenotype

(Chang and Neufeld, 2009)

Phenotype Manifest In

Suppressor of

Statement

Reference

Atg13^UAS.cCa, Scer\GAL4^Cg.PA is a suppressor of autophagosome | larval stage phenotype of Aduk^UAS.EGFP, Scer\GAL4^Cg.PA

(Braden and Neufeld, 2016)

Atg13^UAS.cCa, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval fat body | larval stage phenotype of Aduk^UAS.EGFP, Scer\GAL4^Cg.PA

(Braden and Neufeld, 2016)

Other

Statement

Reference

Atg13^UAS.cCa, Atg1^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype

(Chang and Neufeld, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of autophagic vesicles (as detected by Atg8a signal) in the fat body of well-fed larvae induced by expression of Aduk^Scer\UAS.cBa under the control of Scer\GAL4^Cg.PA is prevented by co-expression of Atg13^Scer\UAS.cCa.

(Braden and Neufeld, 2016)

The pupariation delay and increased pupal size observed in flies expressing Npc1a^KK101077 under the Scer\GAL4^P0206 driver is rescued by simultaneous co-expression of Atg1^Scer\UAS.cSa and Atg13^Scer\UAS.cCa.

(Danielsen et al., 2016)

Co-expression of Atg13^Scer\UAS.cCa results in a marked increase in the number, size, and distribution of autophagosomes in animals expressing Atg1^{Scer\UAS.T:Hsap\MYC} under the control of low levels of Scer\GAL4^hs.PB (using a 1 hour heat shock).

Co-expression of Atg13^Scer\UAS.cCa and Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP in fat body cell clones results in cells that are markedly reduced in size compared to neighbouring control cells.

Co-expression of Atg13^Scer\UAS.cCa and Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF leads to disruption of ommatidial patterning.

Co-expression of Atg13^Scer\UAS.cCa and Atg1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Cg.PA leads to a growth and developmental arrest at the first instar stage of development.

(Chang and Neufeld, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Atg13^Scer\UAS.cCa

Atg13^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Chang

Secondary FlyBase IDs

References (3)

Report Sections

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