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General Information
Symbol
Dmel\Ddcts2
Species
D. melanogaster
Name
FlyBase ID
FBal0002299
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ddcts2/Ddc7 and Ddcts2/Df(2L)TW130 adults raised at 18[o]C and then transferred to 25[o]C 4 days prior to infection show no difference in survival rates after infection with either E. coli or S. aureus compared to the survival rates of wild-type controls.

When Ddcts2 pharate adult tissue (derived from larvae reared at the permissive temperature until third instar and then shifted to the restrictive temperature) is incubated with dopamine in vitro, wild-type levels of melanisation are obtained.

Without heat shock individuals carrying P{Hsp70/Ddc} exhibit full suppression of the pupal cuticle colouration phenotype.

Homozygous larvae raised at the restrictive temperature (29oC) show a significant decrease in immune capacity compared to homozygous larvae raised at the permissive temperature (20oC), with less than 9% melanotic encapsulation of L.boulardi eggs 48 hours after parisitization for larvae raised at 29oC, compared to approximately 80% encapsulation for larvae raised at 20oC.

Recently eclosed hemizygous adults shifted to 29oC have an underpigmented cuticle which darkens on aging, producing abnormally dark adults with obvious concentrations of melanisation in the joints of the legs and axillae of the wings. The bristles of hemizygous adults shifted to 29oC during metamorphosis are pale and straw coloured. Hemizygous pupal cases are dark grey and flexible.

Few homozygotes eclose at 22oC and exhibit an incomplete sclerotization phenotype.

Homozygotes are lethal at 25oC or 29oC and viable at 22oC. Adult escapers have a pale unpigmented cuticle, unpigmented bristles, melanization of joints on the leg and axillae of the wing. Phenotype can be rescued by introduction of P{DdcP10} construct.

At 25oC pupae are yellow-green.

Shows the escaper phenotype (incomplete sclerotinization) when transheterozygous with Df(2L)TW130. Developmental time is protracted by at least four days.

Shows conventional temperature sensitive effects on viability, though does not show sterility at 30oC.

Specific DDC activity of newly eclosed adult CyO/Ddc heterozygotes, as percentage of CyO/+ control: 52.3

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Cuticle phenotype is rescued by Ddc+t7.5.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Wright.

Comments
Comments

Induced by ethyl methanesulfonate or formaldehyde.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (20)