The expression of LamCG489V.UAS under the control of Scer\GAL4fln.IFM, or Scer\GAL4Act88F.PB leads to a severe and progressive decrease in flight ability, until individuals are unable to fly; expression under the control of Scer\GAL4EDTP-DJ694 leads to a milder decrease in flight ability; the Scer\GAL4fln.IFM-driven flight defects are also observed in a LamCEX296 heterozygous background.
Expressing LamCG489V.UAS under the control of Scer\GAL4Act88F.PB leads to structural defects in pupal indirect flight muscles, with shorter sarcomeres, and disrupted Z-discs and M-lines.
Adult indirect flight muscles expressing LamCG489V.UAS under the control of either Scer\GAL4fln.IFM or Scer\GAL4EDTP-DJ694 show myofibril disorganization, abnormal nuclear morphology, including nuclear envelope blebbing, and nuclear misalignment. The Scer\GAL4fln.IFM-driven expression also leads to shorter sarcomeres, with partial loss of Z-disc and M-lines, to severe mitochondrial dysmorphology including small and fragmented mitochondria, and to double membrane structures containing mitochondria and vacuoles, suggesting autophagic defects; adults show a nearly fully penetrant 'held-up wing' phenotype.
Expression of LamCG489V.Scer\UAS under the control of Scer\GAL4C57 leads to larval locomotion defects and semi-lethality at the pupal stage. A subset of the larval body wall muscles show abnormally shaped and spaced nuclei, and disorganization of the actin cytoskeleton.
Third instar larvae expressing LamCG489V.Scer\UAS under the control of Scer\GAL4C57 show reduced mobility. Myonuclei appear aggregated in approximately 30% of body wall muscles.