FlyBase Allele Report: Dmel\LamC[EX296]

General Information

Symbol

Dmel\LamC^EX296

Species

D. melanogaster

Name

FlyBase ID

FBal0192074

Feature type

allele

Associated gene

Dmel\LamC

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Schulze et al., 2005)

Progenitor genotype

P{PTT-GB}LamC^G00158

(Schulze et al., 2005)

Caused by aberration

Df(2L)LamC^EX296

Cytology

Description

Imprecise excision of the P{PTT-GB} element, removing the P{PTT-GB} insertion and resulting in a 560bp deletion in the first LamC exon. This lesion is located within both the LamC and ttv transcription units (since LamC is nested within an intron of ttv). However, only LamC function is affected, since the "EX296" chromosome complements ttv^00681b and fails to complement LamC^EP2199.

(Schulze et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with Df(2R)trix)

(Schulze et al., 2005)

abnormal flight | progressive, with LamC^A177P.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

flightless | progressive, with LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

flightless | progressive, with LamC^R205W.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

lethal (with LamC^EP2199)

(Schulze et al., 2005)

lethal (with LamC^EX3)

(Schulze et al., 2005)

lethal (with LamC^EX5)

(Schulze et al., 2005)

lethal (with LamC^EX16)

(Schulze et al., 2005)

lethal (with LamC^EX187)

(Schulze et al., 2005)

lethal (with LamC^EX265)

(Li et al., 2016, Schulze et al., 2005)

lethal (with LamC^EX364)

(Schulze et al., 2005)

lethal (with LamC^G00158)

(Schulze et al., 2005)

lethal (with LamC^UM-8373-3)

(Schulze et al., 2005)

lethal | recessive

(Schulze et al., 2005)

lethal - all die before end of first instar larval stage | recessive

(Gurudatta et al., 2010)

lethal - all die before end of prepupal stage (with Df(2R)trix)

(Schulze et al., 2005)

lethal - all die before end of P-stage

(Uchino et al., 2013)

lethal - all die before end of P-stage (with Df(2R)trix)

(Gurudatta et al., 2010)

some die during prepupal stage | recessive (with Df(2R)trix)

(Schulze et al., 2005)

some die during P-stage

(Uchino et al., 2013)

some die during third instar larval stage (with Df(2R)trix)

(Gurudatta et al., 2010)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-72

(Gurudatta et al., 2010)

suppressor of variegation | dominant, with T(2;3)Sb^V

(Gurudatta et al., 2010)

Phenotype Manifest In

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with LamC^EX265)

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with LamC^EX265), with LamC^E174K.UAS, Scer\GAL4^BG487

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with LamC^EX265), with LamC^R564C.UAS, Scer\GAL4^BG487

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 4 | third instar larval stage (with LamC^EX265)

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 4 | third instar larval stage (with LamC^EX265), with LamC^E174K.UAS, Scer\GAL4^BG487

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 4 | third instar larval stage (with LamC^EX265), with LamC^R564C.UAS, Scer\GAL4^BG487

(Li et al., 2016)

NMJ bouton | larval stage (with LamC^EX265)

(Li et al., 2016)

nuclear lamina | third instar larval stage (with LamC^EX265), with LamC^E174K.UAS, Scer\GAL4^BG487

(Li et al., 2016)

nuclear lamina | third instar larval stage (with LamC^EX265), with LamC^R564C.UAS, Scer\GAL4^BG487

(Li et al., 2016)

Detailed Description

Statement

Reference

LamC^EX296/LamC^EX265 transheterozygotes are lethal before adulthood and their third instar larval muscles 6 and 7 exhibit a significantly increased proportion of 'ghost' neuromuscular junctions, but not of abnormal nuclear lamina blebs, as compared to controls.

The expression of either LamC^{E174K.Scer\UAS} or LamC^{R564C.Scer\UAS} under the control of Scer\GAL4^Mhc.PW in LamC^EX296/LamC^EX265 transheterozygotes leads to a strong increase in the number of nuclei exhibiting more than 10 abnormal lamina blebs in third instar larval muscles 6 and 7, as compared to controls.

(Li et al., 2016)

Many homozygotes raised on sterilised corn-meal medium at 24[o]C and places on sterilised 20% agar plates at the pre-pupal stage can survive to the late pupal stage. Adult body formation is seen within the pupal cases, but none complete head eversion and no adults eclose.

(Uchino et al., 2013)

LamC^EX296 is early larval lethal when homozygous and late larval or early pupal lethal when hemizygous with Df(2R)trix.

Heterozygous LamC^EX296 suppresses the position effect variegation of P{hsp26-pt-T}39C-72.

Heterozygous LamC^EX296 suppresses the position effect variegation of T(2;3)Sb^V.

(Gurudatta et al., 2010)

LamC^EX296/Df(2R)trix animals show a developmental delay of about 2 days for all stages.

(Schulze et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

In(1)w^m4h, LamC^EX296, Su(var)205⁵/Su(var)205[+] has suppressor of variegation | dominant phenotype

(Gurudatta et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

In(1)w^m4h; Su(var)205⁵/LamC^EX296 flies have nearly normal red eye colour with little evidence of variegation.

(Gurudatta et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

LamC^EX364

(Schulze et al., 2005)

LamC^EX265

(Schulze et al., 2005)

LamC^EX187

(Schulze et al., 2005)

LamC^EX3

(Schulze et al., 2005)

LamC^EX5

(Schulze et al., 2005)

LamC^EX16

(Schulze et al., 2005)

LamC^UM-8373-3

(Schulze et al., 2005)

LamC^G00158

(Schulze et al., 2005)

LamC^EP2199

(Schulze et al., 2005)

Rescued by

LamC^EX296/LamC^EX265 is rescued by Scer\GAL4^Mhc.PW/LamC^UAS.cLa

(Li et al., 2016)

Not rescued by

LamC^EX296/LamC^EX265 is not rescued by Scer\GAL4^Mhc.PW/LamC^E174K.UAS

(Li et al., 2016)

LamC^EX296/LamC^EX265 is not rescued by Scer\GAL4^Mhc.PW/LamC^R564C.UAS

(Li et al., 2016)

Comments

LamC^EX296/LamC^EX265 transheterozygotes exhibit lethality before adulthood, which is not rescued by the expression of LamC^{R564C.Scer\UAS} under the control of Scer\GAL4^Mhc.PW; LamC^EX296/LamC^EX265 transheterozygotes also exhibit an increased proportion of 'ghost' neuromuscular junctions in third instar larval muscles 6 and 7, which is rescued by the expression of LamC^Scer\UAS.cLa, but not of LamC^{E174K.Scer\UAS} or LamC^{R564C.Scer\UAS}, under the control of Scer\GAL4^Mhc.PW.

(Li et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

605590

w^*; LamC^EX296/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

LamC^EX296

(Chandran et al., 2019, Uchino et al., 2013)

LamC^Ex296

(Wang et al., 2018)

lamC^EX296

(Li et al., 2016, Gurudatta et al., 2010)

lamin C ^Ex296

(Rohrbaugh et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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