FlyBase Allele Report: Dmel\LamC[EX187]

General Information

Symbol

Dmel\LamC^EX187

Species

D. melanogaster

Name

FlyBase ID

FBal0192072

Feature type

allele

Associated gene

Dmel\LamC

Associated Insertion(s)

P{?PTT-GB}LamC^EX187

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Schulze et al., 2005)

Progenitor genotype

P{PTT-GB}LamC^G00158

(Schulze et al., 2005)

Associated Insertion(s)

P{?PTT-GB}LamC^EX187

Cytology

Description

Imprecise excision of the P{PTT-GB} element, resulting in a 357bp deletion in the first LamC exon. 2 insertions appear to be present (Avic\GFP sequences from the P{PTT-GB} element are detected but functional w[+] sequences are not present). The lesion is located within both the LamC and ttv transcription units (since LamC is nested within an intron of ttv). However, only LamC function is affected by the lesion, since the "EX187" chromosome complements ttv^00681b and fails to complement LamC^EP2199.

(Schulze et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2R)trix)

(Schulze et al., 2005)

lethal (with LamC^EP2199)

(Schulze et al., 2005)

lethal (with LamC^EX3)

(Schulze et al., 2005)

lethal (with LamC^EX5)

(Schulze et al., 2005)

lethal (with LamC^EX16)

(Schulze et al., 2005)

lethal (with LamC^EX265)

(Schulze et al., 2005)

lethal (with LamC^EX296)

(Schulze et al., 2005)

lethal (with LamC^EX364)

(Schulze et al., 2005)

lethal (with LamC^G00158)

(Schulze et al., 2005)

lethal (with LamC^UM-8373-3)

(Schulze et al., 2005)

lethal | recessive

(Schulze et al., 2005)

lethal - all die before end of first instar larval stage | recessive

(Gurudatta et al., 2010)

lethal - all die before end of P-stage

(Uchino et al., 2013)

lethal - all die before end of P-stage (with Df(2R)trix)

(Gurudatta et al., 2010)

some die during P-stage

(Uchino et al., 2013)

some die during third instar larval stage (with Df(2R)trix)

(Gurudatta et al., 2010)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-72

(Gurudatta et al., 2010)

suppressor of variegation | dominant, with T(2;3)Sb^V

(Gurudatta et al., 2010)

Phenotype Manifest In

embryonic/larval salivary gland

(Schulze et al., 2009)

imaginal disc

(Schulze et al., 2009)

nuclear envelope | larval stage

(Schulze et al., 2009)

nuclear inner membrane | larval stage

(Schulze et al., 2009)

nuclear outer membrane | larval stage

(Schulze et al., 2009)

nuclear pore | larval stage

(Schulze et al., 2009)

nucleus | larval stage

(Schulze et al., 2009)

Detailed Description

Statement

Reference

Many homozygotes raised on sterilised corn-meal medium at 24[o]C and places on sterilised 20% agar plates at the pre-pupal stage can survive to the late pupal stage. Adult body formation is seen within the pupal cases, but none complete head eversion and no adults eclose.

(Uchino et al., 2013)

LamC^EX187 is early larval lethal when homozygous and late larval lethal or early pupal lethal when hemizygous with Df(2R)trix.

Heterozygous LamC^EX187 suppresses the position effect variegation of P{hsp26-pt-T}39C-72.

Heterozygous LamC^EX187 suppresses the position effect variegation of T(2;3)Sb^V.

(Gurudatta et al., 2010)

LamC^EX187 mutant larval salivary gland nuclei show clustering of the nuclear pores. 15% of larval imaginal disc nuclei show separation of the inner and outer nuclear membranes and large perforations in the nuclear envelope resulting in chromatin leakage. The larval body wall muscles show abnormally shaped nuclei.

(Schulze et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

In(1)w^m4h, LamC^EX187, Su(var)205⁵/Su(var)205[+] has suppressor of variegation | dominant phenotype

(Gurudatta et al., 2010)

Phenotype Manifest In