Gene model reviewed during 5.46
Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.
None of the polypeptides share 100% sequence identity.
Interacts with MAN1.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\LamC using the Feature Mapper tool.
In Northern blots, LamC transcript is detected starting in 12 hr embryos, and peaks in 19-22 hr embryos. Expression declines after larval stages, and is low in pupal and larval stages. Using in situ hybridization, LamC is first detected in late stage 12 embryos, in oenocytes. At stage 13, expression is detected in oenocytes, the hindgut, the foregut, and in the dorsal longitudinal trunks.
LamC protein is first detected in 12-15 hr embryos, reaches peak levels in larval stages, and is at low levels in pupal and adult stages. In embryos, LamC protein is first detected in late stage 12, in oenocytes, in the posterior spiracle, and in the hindgut. At stage 15, expression is detected in the foregut, the epidermis, and the dorsal longitudinal trunks. At stage 16, LamC protein is detected in the dorsal pharyngeal musculature, in exit glia, and in the visceral mesoderm. In larvae, LamC protein is detected in all nuclei, but at varying levels.
GBrowse - Visual display of RNA-Seq signalsView Dmel\LamC in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Overexpression of LamC during most larval stages stalls growth, inhibits ecdysteroid signaling, causes melanotic tumours and results in lethality.
Biochemical techniques reveal that in living cells lamins are closely associated with both DNA and RNA.
Clone pG-IF (isolated in FBrf0058818) encodes a nuclear lamin whose expression is developmentally controlled.
LamC gene locus organisation has been determined and its structure compared to the vertebrate lamin genes.
G-IF encodes a 46kD intermediate filament protein with around 60% nucleotide identity to Lam.