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General Information
Symbol
Dmel\ThorUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Miron
FlyBase ID
FBal0147361
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-4E-BP, UAS-Thor, UAS-4E-BPwt
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild-type Thor.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of ThorScer\UAS.cMa under the control of Scer\GAL4da.PU does not induce mitochondria morphology defects in adult indirect flight muscles, as compared to controls; expression under the control of Scer\GAL4Ddc.PU does not induce significant defects in the adult life-span, in the adult locomotor capacity or in the number of dopaminergic PAL, PPM1/2, PPM3, PPL1 or PPL2 neurons in the adult brain, as compared to controls.

Ectopic expression of ThorScer\UAS.cMa under the Scer\GAL4elav-C155 driver does not alter the number of neuromuscular junction boutons in third instar larvae compared to controls.

Expression of ThorScer\UAS.cMa under the control of Scer\GAL4Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Expression of ThorScer\UAS.cMa under the simultaneous control of both Scer\GAL4Scer\UAS.cHa and Scer\GAL4tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

Post-synaptic expression of ThorScer\UAS.cMa under the control of Scer\GAL4Mhc.PW leads to a decrease in the total number of type I boutons (both type Ib and type Is) on muscle 6/7 of A3. Presynaptic expression of ThorScer\UAS.cMa (under the control of Scer\GAL4elav-C155) has no effect.

Overexpression of ThorScer\UAS.cMa under the control of Scer\GAL4arm.PS does not change lifespan on rich food in males or females. Under dietary restriction, there is no lifespan extension in these flies

Flies expressing ThorScer\UAS.cMa under the control of Scer\GAL4da.G32 are more resistant to hydrogen peroxide compared to control flies.

Overexpression of ThorScer\UAS.cMa, under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C has no effect on fat body internalisation of TR-avidin.

Expression of ThorScer\UAS.cMa under the control of Scer\GAL4Bx-MS1096 has no effect on wing size or pattern. Expression of ThorScer\UAS.cMa under the control of Scer\GAL4GMR.PF does not result in any discernible phenotype in the eye.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of LamCUAS.cSa and ThorUAS.cMa under the control of Scer\GAL4fln.IFM under the control of Scer\GAL4fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

S6kl-1 homozygous somatic clones also expressing ThorScer\UAS.cMa under the control of Scer\GAL4αTub84B.PL in third instar larval eye discs do not exhibit any delay in G1/S transition during the second mitotic wave, as mutant cells enter S phase in a similar region to control cells.

The expression of ThorScer\UAS.cMa under the control of Scer\GAL4da.PU suppresses the frequency and severity of the mitochondria morphology defects displayed in Chchd2H43 homozygous adult indirect flight muscles. Expression under the control of Scer\GAL4Ddc.PU suppresses the decreased life-span, the locomotor defects and the decreased number of dopaminergic PPL1 neurons in the brain, presented by Chchd2H43 homozygous adults; this expression, however, does not significantly affect the numbers of dopaminergic PAL, PPM1/2, PPM3 or PPL2 neurons in the adult brain of Chchd2H43 homozygotes.

Expression of ThorScer\UAS.cMa under the control of Scer\GAL4da.PU also suppresses the mitochondria morphology defects in adult indirect flight muscle exhibited by Chchd2null homozygotes.

Expression of ThorScer\UAS.cMa under the control of Scer\GAL4ey-OK107 fails to suppress the axon degeneration seen in bskflp147E mutant mushroom body neuron clones.

Expression of Sod2Scer\UAS.cUa or ThorScer\UAS.cMa driven by Scer\GAL4hs.PU partially suppresses phenotypes (collapsed thorax, downturned wings, reduced levels of mtDNA and ATP in the indirect flight muscle, and locomotor defects) seen in Pink1B9/Y flies. Expression of Sod2Scer\UAS.cUa or ThorScer\UAS.cMa driven by Scer\GAL4ple.PF partially suppresses the enlarged mitochondria in dopaminergic neurons and dopaminergic neuron loss (DL1 cluster) seen in Pink1B9/Y flies.

The reduction of neuromuscular junctions caused by post-synaptic expression of LrrkScer\UAS.cIa (under the control of Scer\GAL4Mhc.PW) is fully suppresses by the coexpression of ThorScer\UAS.cMa.

Coexpression of ThorScer\UAS.cMa in the post-synaptic compartment under the control of Scer\GAL4Mhc.PW is unable to rescue the neuromuscular junction phenotype caused by presynaptic LrrkScer\UAS.cIa expression (under the control of Scer\GAL4elav-C155.

Thor2/Thor2; park25/park25 double mutants are almost completely lethal and Thor2/Thor2; Pink1B9/Pink1B9 double mutants are partially lethal (unlike either viable single mutant or viable single mutants with the other allele heterozygotic), with rare escapers dying soon after eclosion; expression of ThorScer\UAS.cMa driven by Scer\GAL4da.PU suppresses lethality in double mutants.

Expression of ThorScer\UAS.cMa driven by Scer\GAL4how-24B significantly partially suppresses flight and climbing defects as well as muscle degeneration and mitochondrial disruption in Pink1B9/Pink1B9 flies. Expression of ThorScer\UAS.cMa driven by Scer\GAL4ple.PF significantly suppresses loss of dopaminergic PPL1 neurons in 30 day old Pink1B9/Pink1B9 flies. Expression of foxoScer\UAS.cFa driven by Scer\GAL4how-24B significantly partially suppresses flight and climbing defects as well as muscle degeneration and mitochondrial disruption in flies. Expression of ThorScer\UAS.cMa or foxoScer\UAS.cFa driven by Scer\GAL4ple.PF significantly suppresses loss of dopaminergic PPL1 neurons in 30 day old park25/park25 flies.

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing LrrkI1915T.Scer\UAS under the control of Scer\GAL4ple.PF is suppressed by co-expression of ThorScer\UAS.cMa.

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing LrrkY1383C.Scer\UAS under the control of Scer\GAL4ple.PF is suppressed by co-expression of ThorScer\UAS.cMa.

The enlarged eye phenotype caused by expression of Akt1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is partially suppressed by co-expression of ThorScer\UAS.cMa.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Ubiquitous expression of ThorScer\UAS.cMa under the control of Scer\GAL4da.G32 rescues the dietary restriction phenotype of Thork13517 mutants from 8% to 37% lifespan extension in males and from 13% to 48% in females, similar to controls.

The increased sensitivity of Thork13517 flies to paraquat and to hydrogen peroxide is rescued by expression of ThorScer\UAS.cMa under the control of Scer\GAL4da.G32.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid, transfected into S2 cells and used in translation assays.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ThorScer\UAS.cMa
ThorUAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Miron
Secondary FlyBase IDs
    References (21)