FlyBase Allele Report: Dmel\Lrrk[Y1383C.UAS]

General Information

Symbol

Dmel\Lrrk^Y1383C.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0220787

Feature type

allele

Associated gene

Dmel\Lrrk

Associated Insertion(s)

Carried in Construct

P{UAS-Lrrk.Y1383C}

Also Known As

UAS-Lrrk Y1383C, UAS-Lrrk^Y1383C

Key Links

Genomic Maps

JBrowse

Transgenic product class

missense_variant

(Imai et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Imai et al., 2008)

Mutagen

in vitro construct

(Imai et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Lrrk.Y1383C}

Cytology

Description

Amino acid replacement: Y1383C.

(Imai et al., 2008)

UAS regulatory sequences drive expression of a mutated form of Lrrk.

(Imai et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Lrrk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:20,640,647..20,640,647 [+]

Comment:

The Y1383C mutation was reported relative to Lrrk-PB. analogous mutation in human LRRK2 implicated in Parkinson;disease 8; mutation carried on in vitro construct; site of nucleotide substitution in fly gene and specific disease association inferred by FlyBase curator.

(Imai et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Godena et al., 2014)

model of Parkinson's disease 8

CEC

(Inoshita et al., 2022)

CEA

(Sarkar et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Bardai et al., 2018)

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^QUAS.cOa

(Sarkar et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Imai et al., 2008)

LRRK2:p.Tyr1699Cys

(FlyBase curators, 2019-)

Variants Synonym(s)

Associated human disease model(s)

Parkinson disease 8

External database links

ClinVar: LRRK2_1937

Comments concerning this variant

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Toll-6-D42

(Godena et al., 2014)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Imai et al., 2008)

chemical sensitive, with Scer\GAL4^da.G32

(Imai et al., 2008)

Phenotype Manifest In

dopaminergic neuron, with Scer\GAL4^ple.PF

(Imai et al., 2008)

Detailed Description

Statement

Reference

Axonal transport of mitochondria is disrupted in larval motor neurons expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^D42.

Flies expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^D42 show locomotor defects.

(Godena et al., 2014)

Flies expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^ple.PG do not show a progressive reduction in electroretinogram amplitude (there is no significant difference in the amplitude seen in 3 and 28 day old flies).

(Hindle et al., 2013)

60 day old adults expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^ple.PF show a significant reduction in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster compared to control flies, although the number of neurons in the protocerebral posterior medial 3 cluster is unaffected in the mutant flies.

Flies expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^da.G32 show significantly higher sensitivity to paraquat and to hydrogen peroxide compared to control flies.

(Imai et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lrrk^Y1383C.UAS, Scer\GAL4^ple.PF has abnormal neuroanatomy | adult stage phenotype, suppressible by Thor^UAS.cMa, Scer\GAL4^ple.PF

(Imai et al., 2008)

Enhancer of

Statement

Reference

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^Y1383C.UAS, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKb

(Kanao et al., 2010)

Other

Statement

Reference

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has short lived | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has abnormal neuroanatomy | adult stage | RU486 conditional phenotype

(Kanao et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lrrk^Y1383C.UAS, Scer\GAL4^ple.PF has dopaminergic neuron phenotype, suppressible by Thor^UAS.cMa, Scer\GAL4^ple.PF

(Imai et al., 2008)

Enhancer of

Statement

Reference

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^Y1383C.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cKb

(Kanao et al., 2010)

Other

Statement

Reference

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPL1 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPM3 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the eye defects caused by expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PF are enhanced by co-expression of Lrrk^{Y1383C.Scer\UAS}.

Co-expression of foxo^Scer\UAS.cKb and Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 throughout the lifespan results in a reduction in lifespan. These flies show an age-dependent decrease in the number of PPM1/2, PPM3 and PPL1 neurons.

(Kanao et al., 2010)

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^ple.PF is suppressed by co-expression of Thor^Scer\UAS.cMa.

(Imai et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lrrk^{Y1383C.Scer\UAS}

Lrrk^Y1383C.UAS

Name Synonyms

Secondary FlyBase IDs

References (8)

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