FlyBase Allele Report: Scer\GAL4[nSyb.PP]

General Information

Symbol

Scer\GAL4^nSyb.PP

Species

S. cerevisiae

Name

n-synaptobrevin promoter construct of Potter

FlyBase ID

FBal0288658

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

M{nSyb-GAL4.P}

P{nSyb-GAL4.P}

Also Known As

nSyb-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Riabinina and Potter, 2013.10.1)

Progenitor genotype

Carried in construct

M{nSyb-GAL4.P}

P{nSyb-GAL4.P}

Cytology

Description

nSyb regulatory sequences control expression of a GAL4 driver.

(Riabinina and Potter, 2013.10.1)

Allele components

Component

Use(s)

Regulatory region(s)

nSyb

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

neuron

(Bloomington Drosophila Stock Center, 2017, Riabinina and Potter, 2013.10.1)

larval stage -- adult stage

neuron

(Riabinina et al., 2015)

adult stage

adult neuron

(Tanaka and Clark, 2022)

Additional Information

Statement

Reference

ScerGAL4^nSyb.PP drives expression pan-neuronally.

(Bloomington Drosophila Stock Center, 2017)

Drives expression pan-neuronally at all developmental stages.

(FlyBase Curators, 2017)

ScerGAL4^nSyb.PP drives pan-neuronal expression.

(Riabinina et al., 2015)

ScerGAL4^nSyb.PP drives pan-neuronal expression at all stages.

(Riabinina and Potter, 2013.10.1)

Marker for

Reflects expression of

nSyb

(Tanaka and Clark, 2022, Bloomington Drosophila Stock Center, 2017, Riabinina et al., 2015, Riabinina and Potter, 2013.10.1)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage, with D12^GD14771

(Lo Piccolo et al., 2024)

abnormal behavior | adult stage, with D12^KK100444

(Lo Piccolo et al., 2024)

abnormal circadian rhythm | adult stage | progressive, with LKRSDH^GL00396

(Lv et al., 2024)

abnormal circadian rhythm | adult stage | progressive, with LKRSDH^HMC03424

(Lv et al., 2024)

abnormal circadian rhythm | adult stage | progressive, with LKRSDH^NIG.7144R

(Lv et al., 2024)

abnormal circadian rhythm | adult stage | progressive, with LKRSDH^TOE.GS01508, Spyo\Cas9^UAS.cUa

(Lv et al., 2024)

abnormal cold stress response | larval stage, with mTor^HMS00904

(Matsumura et al., 2025)

abnormal cold stress response | larval stage, with z^KK110032

(Matsumura et al., 2025)

abnormal heat stress response | larval stage, with BacA\p35^UAS.cHa

(Matsumura et al., 2025)

abnormal heat stress response | larval stage, with mTor^HMS00904

(Matsumura et al., 2025)

abnormal heat stress response | larval stage, with Phae1^KK106428

(Matsumura et al., 2025)

abnormal heat stress response | larval stage, with Phae1^NIG.16996R

(Matsumura et al., 2025)

abnormal heat stress response | larval stage, with z^HMJ21949

(Matsumura et al., 2025)

abnormal heat stress response | larval stage, with z^KK110032

(Matsumura et al., 2025)

abnormal locomotor behavior | adult stage, with fwd^GL00145

(Terriente-Felix et al., 2020)

abnormal mating behavior, with for^HMS04486

(Miao et al., 2025)

abnormal osmotic stress response | larval stage, with mTor^HMS00904

(Matsumura et al., 2025)

abnormal osmotic stress response | larval stage, with z^KK110032

(Matsumura et al., 2025)

abnormal stress response | larval stage, with mTor^HMS00904

(Matsumura et al., 2025)

abnormal stress response | larval stage, with z^KK110032

(Matsumura et al., 2025)

bang sensitive, with D12^GD14771

(Lo Piccolo et al., 2024)

bang sensitive, with D12^KK100444

(Lo Piccolo et al., 2024)

bang sensitive, with Imp^HMC03794

(Roy and Link, 2025)

bang sensitive, with Sdc^KK108799

(Roy and Link, 2025)

decreased rate of adult locomotory behavior, with D12^GD14771

(Lo Piccolo et al., 2024)

decreased rate of adult locomotory behavior, with D12^KK100444

(Lo Piccolo et al., 2024)

decreased rate of adult locomotory behavior, with Ero1L^UAS.cTa

(Yeewa et al., 2024)

decreased rate of movement | adult stage, with D12^GD14771

(Lo Piccolo et al., 2024)

decreased rate of movement | adult stage, with D12^KK100444

(Lo Piccolo et al., 2024)

decreased size | larval stage, with adp^HMC06600

(Losurdo et al., 2024)

decreased sleep | adult stage, with mir-ban^{sponge.UAS.DsRed(Unk)}

(Hobin et al., 2022)

increased sleep | adult stage, with mir-ban^UAS.cUa

(Hobin et al., 2022)

lethal - all die before end of first instar larval stage, with Hsap\NFIA^WT.UAS

(Uehara et al., 2021)

long lived, with Ero1L^HMC05709

(Yeewa et al., 2024)

partially lethal - majority die | male, with dths^HMS03377

(Zhang et al., 2024)

partially lethal - majority live, with Hsap\MRTFB^{R104G.UAS.Tag:HA}

(Andrews et al., 2023)

short lived, with Ero1L^UAS.cTa

(Yeewa et al., 2024)

short lived, with mtd^HMS01666

(Wang et al., 2019)

some die during embryonic stage, with Hsap\NFIA^WT.UAS

(Uehara et al., 2021)

some die during first instar larval stage, with Hsap\NFIA^WT.UAS

(Uehara et al., 2021)

viable, with Hsap\MRTFB^{A91P.UAS.Tag:HA}

(Andrews et al., 2023)

viable, with Hsap\MRTFB^{E109Q.UAS.Tag:HA}

(Andrews et al., 2023)

viable, with Hsap\MRTFB^{N95S.UAS.Tag:HA}

(Andrews et al., 2023)

viable, with Hsap\MRTFB^UAS.Tag:HA

(Andrews et al., 2023)

viable | female, with dths^HMS03377

(Zhang et al., 2024)

Phenotype Manifest In

larval brain | larval stage, with adp^HMC06600

(Losurdo et al., 2024)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nSyb.PP, z^KK110032 has abnormal heat stress response | larval stage phenotype, suppressible by Phae1^UAS.cMa, Scer\GAL4^nSyb.PP

(Matsumura et al., 2025)

Scer\GAL4^nSyb.PP, mTor^HMS00904 has abnormal heat stress response | larval stage phenotype, suppressible by Phae1^UAS.cMa, Scer\GAL4^nSyb.PP

(Matsumura et al., 2025)

Imp^HMC03794, Scer\GAL4^nSyb.PP has bang sensitive phenotype, suppressible by Sdc^UAS.cJa, Scer\GAL4^nSyb.PP

(Roy and Link, 2025)

Enhancer of

Statement

Reference

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^I1915T.UAS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^I1915T.UAS is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^G1914S.UAS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^G1914S.UAS is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^UAS.cIa is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^UAS.cIa is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^R1069G.UAS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^R1069G.UAS is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^RNAi.UAS.cIa/Scer\GAL4^nSyb.PP is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^RNAi.UAS.cIa/Scer\GAL4^nSyb.PP is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Suppressor of

Statement

Reference

Phae1^UAS.cMa, Scer\GAL4^nSyb.PP is a suppressor of abnormal heat stress response | larval stage phenotype of Scer\GAL4^nSyb.PP, z^KK110032

(Matsumura et al., 2025)

Phae1^UAS.cMa, Scer\GAL4^nSyb.PP is a suppressor of abnormal heat stress response | larval stage phenotype of Scer\GAL4^nSyb.PP, mTor^HMS00904

(Matsumura et al., 2025)

Sdc^UAS.cJa, Scer\GAL4^nSyb.PP is a suppressor of bang sensitive phenotype of Imp^HMC03794, Scer\GAL4^nSyb.PP

(Roy and Link, 2025)

Lrrk^Q1003H.UAS/Scer\GAL4^nSyb.PP is a suppressor of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^Q1003H.UAS/Scer\GAL4^nSyb.PP is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^nSyb.PP/Lrrk^I1915T.UAS is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^I1915T.UAS is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^I1915T.UAS is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^G1914S.UAS is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^G1914S.UAS is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^G1914S.UAS is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^UAS.cIa is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^UAS.cIa is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^UAS.cIa is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^Y1383C.UAS is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^R1069G.UAS is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^R1069G.UAS is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^R1069G.UAS is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Hsap\LRRK2^UAS.cVa/Scer\GAL4^nSyb.PP is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^RNAi.UAS.cIa/Scer\GAL4^nSyb.PP is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^RNAi.UAS.cIa/Scer\GAL4^nSyb.PP is an enhancer of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^RNAi.UAS.cIa/Scer\GAL4^nSyb.PP is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^nSyb.PP/tsr^{UAS.Tag:polyHis} is a suppressor | partially of outer medulla | adult stage phenotype of Lrrk^e03680

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/tsr^{UAS.Tag:polyHis} is a suppressor | partially of autophagosome | increased number phenotype of Lrrk^e03680

(Sarkar et al., 2021)

Lrrk^Q1003H.UAS/Scer\GAL4^nSyb.PP is a suppressor of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^Q1003H.UAS/Scer\GAL4^nSyb.PP is a suppressor of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^Q1003H.UAS/Scer\GAL4^nSyb.PP is a suppressor of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is a suppressor of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is a suppressor of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is a suppressor of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^nSyb.PP/z^UAS.ORF-CC rescues z^KK110032

(Matsumura et al., 2025)

Phae1^UAS.cMa/Scer\GAL4^nSyb.PP rescues Phae1^KK106428

(Matsumura et al., 2025)

Fails to rescue

Pgr^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^nSyb.PP fails to rescue Pgr^A1/Pgr^B1

(Fujinaga et al., 2025)

Scer\GAL4^nSyb.PP/cPges^{UAS.Tag:FLAG,Tag:HA} fails to rescue cPges^KO

(Fujinaga et al., 2025)

Scer\GAL4^nSyb.PP, LKRSDH^TOE.GS01508, Spyo\Cas9^UAS.cUa fails to rescue LKRSDH^MB01942

(Lv et al., 2024)

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

68222

w¹¹¹⁸; M{nSyb-GAL4.P}ZH-86Fb/TM6B

51941

y¹ w¹¹¹⁸; P{nSyb-GAL4.P}attP2

90901

P{hsFLP}1, P{tubP-GAL80}LL1 w^* P{neoFRT}19A; P{nSyb-GAL4.P}attP2

51944

w¹¹¹⁸; βTub60D^Pin-1/CyO; P{nSyb-GAL4.P}attP2, P{UAS-mCD8::GFP.L}LL6

51943

y¹ w¹¹¹⁸; βTub60D^Pin-1/CyO; P{nSyb-GAL4.P}attP2, P{UAS-lacZ.NZ}J312/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^n-syb.PP

Scer\GAL4^nSyb.PP

Name Synonyms

n-synaptobrevin promoter construct of Potter

Secondary FlyBase IDs

References (58)

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