FlyBase Allele Report: Ncra\QF[QF2.nSyb]

General Information

Symbol

Ncra\QF^QF2.nSyb

Species

N. crassa

Name

FlyBase ID

FBal0288660

Feature type

allele

Associated gene

Ncra\QF

Associated Insertion(s)

Carried in Construct

M{nSyb-QF2.P}

P{nSyb-QF2.P}

Also Known As

nsyb-QF2, n-syb-QF2, Syb-QF2

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Riabinina et al., 2015, Riabinina and Potter, 2013.10.1)

in vitro construct - deletion

(Riabinina et al., 2015)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

M{nSyb-QF2.P}

P{nSyb-QF2.P}

Cytology

Nature of the lesion

Statement

Reference

nSyb promoter sequences regulate expression of the QF2 driver (a derivative of QF in which the middle domain is completely deleted).

(Riabinina et al., 2015)

nSyb regulatory sequences control expression of a QF2 driver (a less toxic variant of QF).

(Riabinina and Potter, 2013.10.1)

Allele components

Product class / Tool use(s)

Regulatory region(s)

nSyb

Encoded product / tool

QF2

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

neuron

(Riabinina et al., 2015)

Additional Information

Statement

Reference

Ncra\QF^QF2.nSyb drives pan-neuronal expression.

(Riabinina et al., 2015)

Marker for

Reflects expression of

nSyb

(Riabinina et al., 2015)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

increased cell death | adult stage, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

lethal | heat sensitive, with shi^ts1.QUAS

(Riabinina et al., 2015)

locomotor behavior defective | adult stage, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

short lived, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

size defective | adult stage, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

viable, with Ncra\QS^αTub84B.PP, shi^ts1.QUAS

(Riabinina et al., 2015)

Phenotype Manifest In

adult brain, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

dopaminergic medulla neuron, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

filamentous actin | adult stage, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

medulla, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

mitochondrion | adult stage, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

neuron | adult stage, with Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has locomotor behavior defective | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Act5C^UASp.R.GFP

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Act5C^UASp.R.GFP

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has locomotor behavior defective | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Suppressed by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has locomotor behavior defective | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has size defective | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has locomotor behavior defective | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has locomotor behavior defective | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has size defective | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has size defective | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Gel^UAS.cDa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has locomotor behavior defective | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has size defective | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Enhancer of

Statement

Reference

Hsap\SNCA^QUAS.cOa/Ncra\QF^QF2.nSyb is an enhancer of locomotor behavior defective | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav-C155

(Ordonez et al., 2018)

Other

Statement

Reference

Hsap\MAPT^UAS.cWa, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb, Scer\GAL4^elav-C155 has viable phenotype

(Ordonez et al., 2018)

Hsap\MAPT^R406W.UAS, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb, Scer\GAL4^elav-C155 has lethal phenotype

(Ordonez et al., 2018)

α-Spec^rg41, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has lethal phenotype

(Ordonez et al., 2018)

α-Spec^lm88, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has lethal phenotype

(Ordonez et al., 2018)

α-Spec^MI11816, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has lethal phenotype

(Ordonez et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, enhanceable by Scer\GAL4^elav-C155/Act5C^UASp.R.GFP

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has medulla phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Suppressed by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Gel^UAS.cDa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (11)

Bloomington

78338

y¹ w¹¹¹⁸; P{nSyb-QF2.P}attP2/TM6B, Tb¹

51955

y¹ w¹¹¹⁸; M{nSyb-QF2.P}ZH-86Fb/TM6B, Tb¹

51959

w¹¹¹⁸; P{nSyb-QF2.P}attP2, P{QUAS-nuclacZ.P}44/TM6B, Tb¹

61674

w^*; P{nSyb-MKII::GAL4DBDo}attP24; P{QUAS(FRT.stop)p65AD::CaM}6, P{nSyb-QF2.P}attP2

61675

w^*; P{nSyb-MKII::nlsLexADBDo}attP24/CyO; P{QUAS(FRT.stop)p65AD::CaM}6, P{nSyb-QF2.P}attP2

61671

w^*; P{nSyb-MKII::GAL4DBDo}attP24, P{QUAS-p65AD::CaM}2, P{UAS-mCD8::GFP.L}LL5/CyO; P{nSyb-QF2.P}attP2, P{tub-QS.P}9B

61673

w^*; P{nSyb-MKIIK11A::GAL4DBDo}attP24, P{QUAS-p65AD::CaM}2, P{UAS-mCD8::GFP.L}LL5/CyO; P{nSyb-QF2.P}attP2, P{tub-QS.P}9B

64348

w^*; P{nSyb-MKII::nlsLexADBDo}attP24, P{QUAS-p65AD::CaM}2, P{13XLexAop2-mCD8::GFP}attP40/CyO; P{nSyb-QF2.P}attP2, P{tub-QS.P}9B

61670

w^* P{10XUAS-IVS-mCD8::RFP}attP18 P{13XLexAop2-mCD8::GFP}su(Hw)attP8; P{nSyb-MKII::GAL4DBDo}attP24, P{QUAS-p65AD::CaM}2/CyO; P{nSyb-QF2.P}attP2 P{tub-QS.P}9B

61672

w^* P{10XUAS-IVS-mCD8::RFP}attP18 P{13XLexAop2-mCD8::GFP}su(Hw)attP8; P{nSyb-MKIIK11A::GAL4DBDo}attP24, P{QUAS-p65AD::CaM}2/CyO; P{nSyb-QF2.P}attP2, P{tub-QS.P}9B

64347

w^* P{10XUAS-IVS-mCD8::RFP}attP18 P{13XLexAop2-mCD8::GFP}su(Hw)attP8; P{nSyb-MKII::nlsLexADBDo}attP24, P{QUAS-p65AD::CaM}2/CyO; P{nSyb-QF2.P}attP2, P{tub-QS.P}9B/TM6B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ncra\QF^QF2.n-syb

Ncra\QF^QF2.nSyb

n-syb-QF2

(Shearin et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (13)

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