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General Information
Symbol
Hsap\LRRK2UAS.cVa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Venderova
FlyBase ID
FBal0244979
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hLRRK2 WT, UAS-hLRRK2
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild-type Hsap\LRRK2.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4Mhc.PW, Scer\GAL4G14 or Scer\GAL4how-24B does not result in any significant difference in amplitude of miniature excitatory junction currents (mEJCs), but does lead to an increase in amplitude of EJCs, and an increase in quantal content of larval neuromuscular junctions, and these phenotypes are present over a range of external calcium concentrations, as compared to controls.

Expression of Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4Mhc.PW does not result in a significant difference in number of synaptic boutons, presynaptic release sites, as compared to controls.

Expression of Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4ple.PU (together with tub-Gal80[ts] to limit the time of expression to adulthood) does not significantly change the number of dopaminergic neurons in the PPM1/2 cluster in the adult brain.

50 day old adults expressing Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4ple.PF at room temperature show loss of dopaminergic neurons in the dorsomedial posterior protocerebral clusters (PPM1/2) and in the dorsolateral posterior protocerebral cluster (PPL1) of the brain compared to control flies of the same age. A similar loss of neurons is seen in 20 day old adults expressing Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4ple.PF at 29[o]C.

Expression of Hsap\LRRK2Scer\UAS.cVa under the control of either Scer\GAL4elav.PU or Scer\GAL4da.G32 at room temperature significantly extends the basal lifespan of flies compared to controls. Expression of Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4elav.PU at 29[o]C has no significant effect on basal lifespan.

Flies expressing Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4da.G32 have a significantly greater number of progeny compared with control flies.

Flies expressing Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4da.G32 at room temperature show a greater sensitivity to chronic exposure to low levels of rotenone then control flies.

Flies expressing Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4GMR.PF at 29[o]C show loss of pigmentation in the eyes. Significantly more black lesions are seen in the mutant eyes than in controls. Ultrastructurally, severe disruption of the regular trapezoidal arrangement of the photoreceptor cells is seen. The cell lattice between photoreceptor cell arrays of different ommatidia is completely absent and the ommatidia are sometimes fused together. Large holes are often seen in the eye sections.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
NOT Enhancer of
NOT Suppressor of
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

S6kl-1/+ fully suppresses, and eIF4E1S058911/+ partially suppresses the increase in excitatory junction current amplitude and quantal content seen in larvae NMJs expressing Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4Mhc.PW.

Expression of Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4how-24B fails to suppress the increased quantal content observed in GluRIIASP16/Df(2L)cl-h4 mutant larval neuromuscular junctions.

Co-expression of Hsap\LRRK2Scer\UAS.cVa fails to suppress the increase in excitatory junction current amplitude and quantal content seen in larvae NMJs expressing LrrkScer\UAS.cIa under the control of Scer\GAL4Mhc.PW.

The ectopic wing margin bristle phenotype characteristic for adult flies expressing SerScer\UAS.cUa under the control of Scer\GAL4dpp.PU is not significantly modified by co-expression of Hsap\LRRK2Scer\UAS.cVa.

Flies expressing DlScer\UAS.cUa under the control of Scer\GAL4dpp.PU and kept at 18[o]C do not display any overt wing phenotype but show notching phenotype upon co-expression of Hsap\LRRK2Scer\UAS.cVa.

The vein thickening and ectopic veins phenotype characteristic for N55e11 mutant adults is not modified by expression of Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4dpp.PU in the mutant background.

The wing notching phenotype characteristic for adults expressing neurΔRING.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.PU is exacerbated by co-expression of Hsap\LRRK2Scer\UAS.cVa.

The severity of the eye defects caused by expression of foxoScer\UAS.cKb under the control of Scer\GAL4GMR.PF are enhanced by co-expression of Hsap\LRRK2Scer\UAS.cVa.

Co-expression of Hsap\PINK1Scer\UAS.cYa does not significantly suppress the loss of pigmentation in the eye, the roughness of the eye surface or the formation of holes in eye sections that are seen in flies expressing Hsap\LRRK2Scer\UAS.cVa under the control of Scer\GAL4GMR.PF at 29[o]C, but the formation of black lesions in the eye is partially suppressed. The loss of interommatidial bristles that is seen in flies expressing Hsap\PINK1Scer\UAS.cYa under the control of Scer\GAL4GMR.PF at 29[o]C is enhanced by co-expression of Hsap\LRRK2Scer\UAS.cVa.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\LRRK2Scer\UAS.cVa
Hsap\LRRK2UAS.cVa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Venderova
Secondary FlyBase IDs
    References (5)