FlyBase Allele Report: Dmel\foxo[UAS.cKb]

General Information

Symbol

Dmel\foxo^UAS.cKb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kanao

FlyBase ID

FBal0267692

Feature type

allele

Associated gene

Dmel\foxo

Associated Insertion(s)

Carried in Construct

P{UAS-foxo.Kb}

Key Links

Transgenic product class

wild_type

(Kanao et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kanao et al., 2010)

Mutagen

in vitro construct

(Kanao et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-foxo.Kb}

Cytology

Description

UAS regulatory sequences drive expression of foxo.

(Kanao et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

foxo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Detailed Description

Statement

Reference

Expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PF results in mild defects in the eye.

Expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 throughout the lifespan has no effect on lifespan.

(Kanao et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, enhanceable by Lrrk^UAS.cIa, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, enhanceable by Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, enhanceable by Diap1^th-1/th[+]

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, enhanceable by Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, enhanceable by Lrrk^R1069G.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, enhanceable by Lrrk^Y1383C.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, enhanceable by Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Suppressed by

Statement

Reference

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has abnormal neuroanatomy | adult stage | RU486 conditional phenotype, suppressible by W[+]/hid¹

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, suppressible by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, suppressible | partially by Dronc^KG02994/Nc[+]

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF, foxo^UAS.cKb has visible phenotype, suppressible | partially by W[+]/hid¹

(Kanao et al., 2010)

Other

Statement

Reference

Lrrk^UAS.cIa, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has abnormal neuroanatomy | adult stage | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has abnormal neuroanatomy | adult stage | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has abnormal locomotor behavior | adult stage | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has short lived | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has abnormal neuroanatomy | adult stage | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has short lived | RU486 conditional phenotype

(Kanao et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, enhanceable by Lrrk^UAS.cIa, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, enhanceable by Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, enhanceable by Diap1^th-1/th[+]

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, enhanceable by Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, enhanceable by Lrrk^R1069G.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, enhanceable by Lrrk^Y1383C.UAS, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, enhanceable by Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Suppressed by

Statement

Reference

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic neuron | RU486 conditional phenotype, suppressible by W[+]/hid¹

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPL1 neuron | RU486 conditional phenotype, suppressible by W[+]/hid¹

(Kanao et al., 2010)

Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, suppressible by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, suppressible | partially by Dronc^KG02994/Nc[+]

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^GMR.PF, foxo^UAS.cKb has eye phenotype, suppressible | partially by W[+]/hid¹

(Kanao et al., 2010)

Other

Statement

Reference

Lrrk^UAS.cIa, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPL1 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^I1915T.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPM3 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^UAS.cIa, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPL1 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^UAS.cIa, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPM3 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPL1 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Lrrk^Y1383C.UAS, Scer\GAL4^{elav.Switch.PO}, foxo^UAS.cKb has dopaminergic PPM3 neuron | RU486 conditional phenotype

(Kanao et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the eye defects caused by expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PF are enhanced by co-expression of either Lrrk^Scer\UAS.cIa, Lrrk^{I1915T.Scer\UAS}, Lrrk^{R1069G.Scer\UAS} or Lrrk^{Y1383C.Scer\UAS}.

The severity of the eye defects caused by expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of Akt1^{Scer\UAS.T:Ivir\HA1}.

Expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^{elav.Switch.PO} in a Lrrk^e03680/Lrrk^e03680 background in the presence of RU486 throughout the lifespan has no effect on lifespan.

Co-expression of foxo^Scer\UAS.cKb and Lrrk^Scer\UAS.cIa under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 throughout the lifespan has no effect on lifespan. These flies show an age-dependent decrease in the number of PPM1/2, PPM3 and PPL1 neurons.

Co-expression of foxo^Scer\UAS.cKb and Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 throughout the lifespan results in a reduction in lifespan. These flies show an age-dependent decrease in the number of PPM1/2, PPM3 and PPL1 neurons.

Co-expression of foxo^Scer\UAS.cKb and Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 throughout the lifespan results in a reduction in lifespan. These flies show an age-dependent decrease in the number of PPM1/2, PPM3 and PPL1 neurons.

The eye phenotype caused by co-expression of foxo^Scer\UAS.cKb and Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^GMR.PF is significantly suppressed if the flies also carry either Nc^KG02994/+ or W¹/+.

The eye phenotype caused by co-expression of foxo^Scer\UAS.cKb and Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by th¹/+.

The degeneration of the PPM1/2 and PPL1 neurons which is seen in flies co-expressing foxo^Scer\UAS.cKb and Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 is suppressed if they alo carry W¹/+.

(Kanao et al., 2010)

Xenogenetic Interactions

Statement

Reference

The severity of the eye defects caused by expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PF are enhanced by co-expression of either Hsap\LRRK2^Scer\UAS.cVa or Hsap\LRRK2^{I2020T.Scer\UAS}.

(Kanao et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

dFoxO WT

(Iyer et al., 2016)

foxo^Scer\UAS.cKb

foxo^UAS.cKb

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kanao

Secondary FlyBase IDs

References (2)

Report Sections

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