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General Information
Symbol
Hsap\LRRK2I2020T.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0244982
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hLRRK2 I2020T
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutant form of Hsap\LRRK2, which contains the amino acid replacement I2020T.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
This allele represents a human variant implicated in disease.
LRRK2:p.Ile2020Thr
Variants Synonym(s)
Associated human disease model(s)
External database links
Comments concerning this variant
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4ple.PF, Scer\GAL4ple.TH-C' or Scer\GAL4ple.TH-D', but not if under the control of Scer\GAL4Ddc.PL or Scer\GAL4Ddc.HL9, leads to a significant decrease in proboscis extension reflex (in starved flies exposed to a moderate sugar stimulus) in 3 to 18 days old adults, as compared to controls; these flies exhibit a significantly slower proboscis extension speed compared to controls. In ad libitum conditions, Scer\GAL4ple.PF-driven expression leads to a significant decrease in lifespan compared to controls.

Expression of Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4Mhc.PW does not result in any significant difference in amplitude of miniature excitatory junction currents (mEJCs), but does lead to an increase in amplitude of EJCs, and an increase in quantal content of larval neuromuscular junctions, as compared to controls.

Expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4GMR.PU at 29[o]C causes a rough eye phenotype with pigmentation deficits (presence of black lesions, significantly increased compared to controls). Expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4Ddc.PU causes significant locomotor deficits (reduced climbing ability) in 5 or 10 (but not 20, when all flies including controls show age-dependent locomotor deficits) day old flies, and significantly shortens lifespan, compared to controls.

Flies expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4ple.PG do not show a progressive reduction in electroretinogram amplitude (there is no significant difference in the amplitude seen in 3 and 28 day old flies).

Expression of Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in any apparent eye degeneration.

Expression of Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4elav.PU has no effect on axonal growth in embryonic development.

10 day old and 50 day old adults expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4ple.PF at room temperature show loss of dopaminergic neurons in the dorsomedial posterior protocerebral clusters (PPM1/2) and in the dorsolateral posterior protocerebral cluster (PPL1) of the brain compared to control flies of the same age. A similar loss of neurons is seen in 20 day old adults expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4ple.PF at 29[o]C.

Expression of Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4da.G32 at room temperature significantly extends the basal lifespan of flies compared to controls, while expression of Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4elav.PU at room temperature or at 29[o]C has no significant effect on basal lifespan.

Flies expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4da.G32 have a significantly greater number of progeny compared with control flies.

Flies expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4da.G32 at room temperature show a greater sensitivity to chronic exposure to low levels of rotenone then control flies.

Flies expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4GMR.PF at 29[o]C show loss of pigmentation in the eyes. Significantly more black lesions are seen in the mutant eyes than in controls. Ultrastructurally, severe disruption of the regular trapezoidal arrangement of the photoreceptor cells is seen. The cell lattice between photoreceptor cell arrays of different ommatidia is completely absent and the ommatidia are sometimes fused together. Large holes are often seen in the eye sections.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
NOT Suppressor of
Phenotype Manifest In
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of Vps26G2008 or Vps35EY14200 significantly suppresses eye phenotypes (presence of black lesions in around 50% of flies) in flies with expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4GMR.PU at 29[o]C. Co-expression of Vps26G2008 significantly suppresses locomotor deficits in flies with expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4Ddc.PU. Co-expression of Vps35EY14200 significantly suppresses locomotor deficits and shortened lifespan in flies with expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4Ddc.PU.

Co-expression of Vps26HMS01769 does not significantly enhance eye phenotypes (presence of black lesions) in flies with expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4GMR.PU. Co-expression of Vps29HMS01877 or Vps35HMS01858 does not significantly enhance locomotor deficits in flies with expression of Hsap\LRRK2I2020T.Scer\UAS driven by Scer\GAL4Ddc.PU.

The severity of the eye defects caused by expression of foxoScer\UAS.cKb under the control of Scer\GAL4GMR.PF are enhanced by co-expression of Hsap\LRRK2I2020T.Scer\UAS.

Co-expression of Hsap\PINK1Scer\UAS.cYa does not significantly suppress the loss of pigmentation in the eye, the roughness of the eye surface or the formation of holes in eye sections that are seen in flies expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4GMR.PF at 29[o]C, but the formation of black lesions in the eye is partially suppressed.

The formation of black lesions in the eye seen in flies expressing Hsap\LRRK2I2020T.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of Hsap\PARK2Scer\UAS.cYa.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\LRRK2I2020T.Scer\UAS
Hsap\LRRK2I2020T.UAS
Name Synonyms
Secondary FlyBase IDs
    References (9)