FlyBase Allele Report: Hsap\PINK1[UAS.cYa]

General Information

Symbol

Hsap\PINK1^UAS.cYa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Yang

FlyBase ID

FBal0239192

Feature type

allele

Associated gene

Hsap\PINK1

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\PINK1.Y}

Also Known As

UAS-hPINK1

Key Links

Transgenic product class

wild_type

(Yang et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yang et al., 2006)

Mutagen

in vitro construct

(Yang et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Hsap\PINK1.Y}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\PINK1 coding sequences.

(Yang et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\PINK1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\LRRK2^I2020T.UAS

(Venderova et al., 2009)

modeled by Hsap\LRRK2^UAS.cVa

(Venderova et al., 2009)

modeled by Pink1^RNAi.UAS.cYa

(Yang et al., 2006)

exacerbates Parkinson's disease

modeled by Hsap\LRRK2^UAS.cVa

(Venderova et al., 2009)

modeled by Hsap\LRRK2^I2020T.UAS

(Venderova et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Expression of Hsap\PINK1^Scer\UAS.cYa results in a complex interaction with the Hsap\LRRK2^Scer\UAS.cVa and Hsap\LRRK2^{I2020T.Scer\UAS} Parkinson's Disease models; some aspects of the eye phenotype (black lesions) are suppressed, whereas others (such as bristle loss) are enhanced.

(Venderova et al., 2009)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Phenotype Manifest In

autophagosome | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

enterocyte | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Venderova et al., 2009)

midgut | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

mitochondrion | prepupal stage | somatic clone, with Scer\GAL4^Act.PU

(Liu et al., 2016)

Detailed Description

Statement

Reference

The expression of Hsap\PINK1^Scer\UAS.cYa under the control of Scer\GAL4^Act.PU in midgut clones results in enterocytes during pupariation exhibiting a significant decrease in the relative mitochondrial content and a significant increase in the relative amount of autophagosomes, as assessed by a Atg8a fluorescent reporter, but no significant change in the transverse cell area, as compared to non-clone enterocytes.

(Liu et al., 2016)

Expression of Hsap\PINK1^Scer\UAS.cYa under the control of Scer\GAL4^GMR.PF at 29[o]C does not cause loss of pigmentation in the eye, but the eyes show some loss and disorganisation of interommatidial bristles.

(Venderova et al., 2009)

Expression of Hsap\PINK1^Scer\UAS.cYa, under the control of Scer\GAL4^ple.PF has no effect on TH+ neuron number.

(Yang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

NOT Suppressor of

Statement

Reference

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\LRRK2^I1122V.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\PINK1^UAS.cYa, Scer\GAL4^Act.PU has midgut | prepupal stage | somatic clone phenotype, enhanceable by Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PINK1^UAS.cYa, Scer\GAL4^Act.PU has enterocyte | prepupal stage | somatic clone phenotype, enhanceable by Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PINK1^UAS.cYa, Scer\GAL4^Act.PU has autophagosome | prepupal stage | somatic clone phenotype, enhanceable by Hsap\PRKN^UAS.cYa, Scer\GAL4^Act.PU

(Liu et al., 2016)

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by Hsap\LRRK2^I1122V.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Suppressor of

Statement

Reference

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PINK1^UAS.cYa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^ple.PF

(Yang et al., 2006)

Scer\GAL4^ple.PF/Hsap\PINK1^UAS.cYa is a suppressor of dopaminergic neuron phenotype of Pink1^RNAi.UAS.cYa

(Yang et al., 2006)

Hsap\PINK1^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Yang et al., 2006)

Hsap\PINK1^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of indirect flight muscle cell phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Yang et al., 2006)

Hsap\PINK1^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of muscle cell of leg depressor muscle phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Yang et al., 2006)

Hsap\PINK1^UAS.cYa, Scer\GAL4^da.G32 is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^da.G32

(Yang et al., 2006)

Hsap\PINK1^UAS.cYa, Scer\GAL4^Mhc.PW is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Yang et al., 2006)

NOT Suppressor of

Statement

Reference

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a non-suppressor of pigment cell phenotype of Hsap\LRRK2^UAS.cVa, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a non-suppressor of pigment cell phenotype of Hsap\LRRK2^I1122V.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\LRRK2^I1122V.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Hsap\PINK1^UAS.cYa, Scer\GAL4^GMR.PF is a non-suppressor of pigment cell phenotype of Hsap\LRRK2^I2020T.UAS, Scer\GAL4^GMR.PF

(Venderova et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The increased relative amount of autophagosomes exhibited during pupariation by enterocytes in midgut clones expressing Hsap\PINK1^Scer\UAS.cYa under the control of Scer\GAL4^Act.PU is enhanced by the clonal co-expression of Hsap\PARK2^Scer\UAS.cYa.

(Liu et al., 2016)

Co-expression of Hsap\PINK1^Scer\UAS.cYa does not significantly suppress the loss of pigmentation in the eye, the roughness of the eye surface or the formation of holes in eye sections that are seen in flies expressing Hsap\LRRK2^Scer\UAS.cVa under the control of Scer\GAL4^GMR.PF at 29[o]C, but the formation of black lesions in the eye is partially suppressed. The loss of interommatidial bristles that is seen in flies expressing Hsap\PINK1^Scer\UAS.cYa under the control of Scer\GAL4^GMR.PF at 29[o]C is enhanced by co-expression of Hsap\LRRK2^Scer\UAS.cVa.

Co-expression of Hsap\PINK1^Scer\UAS.cYa does not significantly suppress the loss of pigmentation in the eye, the roughness of the eye surface or the formation of holes in eye sections that are seen in flies expressing Hsap\LRRK2^{I1122V.Scer\UAS} under the control of Scer\GAL4^GMR.PF at 29[o]C. The loss of interommatidial bristles that is seen in flies expressing Hsap\PINK1^Scer\UAS.cYa under the control of Scer\GAL4^GMR.PF at 29[o]C is enhanced by co-expression of Hsap\LRRK2^{I1122V.Scer\UAS}.

(Venderova et al., 2009)

Mitochondrial integrity in Pink1^{dsRNA.Scer\UAS} (under the control of either Scer\GAL4^da.G32 or Scer\GAL4^Mhc.PW) mutants is restored through co-expression of Hsap\PINK1^Scer\UAS.cYa.

Mitochondrial integrity in Pink1^{dsRNA.Scer\UAS} (under the control of either Scer\GAL4^da.G32 or Scer\GAL4^Mhc.PW) mutants is not restored through co-expression of Hsap\PINK1^ΔC.Scer\UAS.

(Yang et al., 2006)

Complementation and Rescue Data

Partially rescues

Scer\GAL4^GH146/Hsap\PINK1^UAS.cYa partially rescues Hsap\PINK1^Pink1.P399L

(Pech et al., 2025)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

95263

w^*; P{UAS-Hsap\PINK1.Y}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\PINK1^Scer\UAS.cYa

Hsap\PINK1^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Yang

Secondary FlyBase IDs

References (4)

Report Sections

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