FlyBase Allele Report: Hsap\SNCA[QUAS.cOa]

General Information

Symbol

Hsap\SNCA^QUAS.cOa

Species

H. sapiens

Name

Neurospora crassa QUAS construct of Ordonez

FlyBase ID

FBal0337971

Feature type

allele

Associated gene

Hsap\SNCA

Associated Insertion(s)

Carried in Construct

P{QUAS-hSNCA.WT}

Also Known As

QUAS-α-synuclein

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Ordonez et al., 2018)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{QUAS-hSNCA.WT}

Cytology

Nature of the lesion

Statement

Reference

QUAS regulatory sequences drive expression of wild-type Hsap\SNCA.

(Ordonez et al., 2018)

Allele components

Product class / Tool use(s)

Regulatory region(s)

QUAS

binary expression system - regulatory region

Encoded product / tool

Hsap\SNCA

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of synucleinopathy

CEA

(Ordonez et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of synucleinopathy

is ameliorated by Fhos^HMJ21037

(Ordonez et al., 2018)

is exacerbated by Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

is ameliorated by Drp1^UAS.cDb

(Ordonez et al., 2018)

is ameliorated by Act5C^G0010

(Ordonez et al., 2018)

is exacerbated by Act5C^UASp.R.GFP

(Ordonez et al., 2018)

is ameliorated by α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

abnormal size | adult stage, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

decreased cell number | adult stage, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

increased cell death | adult stage, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

short lived, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Phenotype Manifest In

adult brain, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

dopaminergic medulla neuron, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

filamentous actin | adult stage, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

medulla, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

mitochondrion | adult stage, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

neuron | adult stage, with Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Detailed Description

Statement

Reference

The expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb leads to significant decreases in adult locomotor activity (in negative geotaxis assays) and in lifespan, and leads to severe neurodegeneration in the adult brain, as shown by the progressive increase in the number of cortical and neuropil vacuoles, progressive loss of neurons in the anterior medulla (namely TH-immunoreactive dopaminergic neurons) and progressive increase in the number of apoptotic neurons, as compared to controls. These adult brains also show a significant and progressive accumulation in the number of rod-shaped actin-rich inclusions and a significantly enlarged and elongated mitochondria, as compared to controls.

(Ordonez et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Act5C^UASp.R.GFP

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Act5C^UASp.R.GFP

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal locomotor behavior | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Suppressed by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal size | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal size | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal size | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Gel^UAS.cDa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has decreased cell number | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has abnormal size | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Enhancer of

Statement

Reference

Hsap\SNCA^QUAS.cOa/Ncra\QF^QF2.nSyb is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav-C155

(Ordonez et al., 2018)

Other

Statement

Reference

Hsap\MAPT^UAS.cWa, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb, Scer\GAL4^elav-C155 has viable phenotype

(Ordonez et al., 2018)

Hsap\MAPT^R406W.UAS, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb, Scer\GAL4^elav-C155 has lethal phenotype

(Ordonez et al., 2018)

α-Spec^rg41, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has lethal phenotype

(Ordonez et al., 2018)

α-Spec^lm88, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has lethal phenotype

(Ordonez et al., 2018)

α-Spec^MI11816, Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has lethal phenotype

(Ordonez et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, enhanceable by Scer\GAL4^elav-C155/Act5C^UASp.R.GFP

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has medulla phenotype, enhanceable by Scer\GAL4^elav-C155/Hsap\MAPT^UAS.cWa

(Ordonez et al., 2018)

Suppressed by

Statement

Reference

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Drp1^UAS.cDb

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has dopaminergic medulla neuron phenotype, suppressible by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/α-Spec^UAS.Tag:MYC

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Gel^UAS.cDa

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has filamentous actin | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has mitochondrion | adult stage phenotype, suppressible | partially by Act5C^G0010/Act5C[+]

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has adult brain phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb has neuron | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Fhos^HMJ21037

(Ordonez et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The adult locomotor activity defects, the adult brain vacuolization, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased number of rod-shaped actin-rich inclusions in the adult brain resulting from the expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb are partially suppressed by either Act5C^G0010 heterozygosity or additional expression of Fhos^HMJ21037 under the control of Scer\GAL4^elav-C155, and are enhanced by the additional expression of Act5C^{Scer\UAS.P\T.R.T:Avic\GFP} under the control of Scer\GAL4^elav-C155. The adult locomotor activity defects, the adult brain vacuolization and the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) in the adult brain resulting from the expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb are partially suppressed by the additional expression of Drp1^Scer\UAS.cDb under the control of Scer\GAL4^elav-C155. The adult locomotor activity defects, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased number of rod-shaped actin-rich inclusions in the adult brain resulting from the expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb are partially suppressed by the additional expression of α-Spec^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155.

The increased mitochondria size in the adult brain of individuals expressing Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb is partially suppressed by either Act5C^G0010 heterozygosity or additional expression of either Fhos^HMJ21037, Drp1^Scer\UAS.cDb, Gel^Scer\UAS.cDa or α-Spec^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155.

The decreased number of anterior medulla cortical neurons resulting from the expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb is enhanced by the additional expression of Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^elav-C155. Co-expressing Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb and Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^elav-C155 leads to more severe adult locomotor defects than either single expression.

The expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb in combination with either heterozygosity for α-Spec^rg41, α-Spec^lm88 or α-Spec^MI11816, or additional expression of Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav-C155 leads to lethality.

(Ordonez et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\SNCA^{Ncra\QUAS.cOa}

Hsap\SNCA^QUAS.cOa

Name Synonyms

Neurospora crassa QUAS construct of Ordonez

Secondary FlyBase IDs

References (4)

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