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General Information
Symbol
Hsap\SNCAQUAS.cOa
Species
H. sapiens
Name
Neurospora crassa QUAS construct of Ordonez
FlyBase ID
FBal0337971
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
QUAS-α-synuclein
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

QUAS regulatory sequences drive expression of wild-type Hsap\SNCA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb leads to significant decreases in adult locomotor activity (in negative geotaxis assays) and in lifespan, and leads to severe neurodegeneration in the adult brain, as shown by the progressive increase in the number of cortical and neuropil vacuoles, progressive loss of neurons in the anterior medulla (namely TH-immunoreactive dopaminergic neurons) and progressive increase in the number of apoptotic neurons, as compared to controls. These adult brains also show a significant and progressive accumulation in the number of rod-shaped actin-rich inclusions and a significantly enlarged and elongated mitochondria, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
Enhancer of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The adult locomotor activity defects, the adult brain vacuolization, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased number of rod-shaped actin-rich inclusions in the adult brain resulting from the expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb are partially suppressed by either Act5CG0010 heterozygosity or additional expression of FhosHMJ21037 under the control of Scer\GAL4elav-C155, and are enhanced by the additional expression of Act5CScer\UAS.P\T.R.T:Avic\GFP under the control of Scer\GAL4elav-C155. The adult locomotor activity defects, the adult brain vacuolization and the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) in the adult brain resulting from the expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb are partially suppressed by the additional expression of Drp1Scer\UAS.cDb under the control of Scer\GAL4elav-C155. The adult locomotor activity defects, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased number of rod-shaped actin-rich inclusions in the adult brain resulting from the expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb are partially suppressed by the additional expression of α-SpecScer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav-C155.

The increased mitochondria size in the adult brain of individuals expressing Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb is partially suppressed by either Act5CG0010 heterozygosity or additional expression of either FhosHMJ21037, Drp1Scer\UAS.cDb, GelScer\UAS.cDa or α-SpecScer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav-C155.

The decreased number of anterior medulla cortical neurons resulting from the expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb is enhanced by the additional expression of Hsap\MAPTScer\UAS.cWa under the control of Scer\GAL4elav-C155. Co-expressing Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb and Hsap\MAPTScer\UAS.cWa under the control of Scer\GAL4elav-C155 leads to more severe adult locomotor defects than either single expression.

The expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb in combination with either heterozygosity for α-Specrg41, α-Speclm88 or α-SpecMI11816, or additional expression of Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4elav-C155 leads to lethality.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\SNCANcra\QUAS.cOa
Hsap\SNCAQUAS.cOa
Name Synonyms
Neurospora crassa QUAS construct of Ordonez
Secondary FlyBase IDs
    References (4)