FlyBase Allele Report: Dmel\Lrrk[3KD.UAS]

General Information

Symbol

Dmel\Lrrk^3KD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0220790

Feature type

allele

Associated gene

Dmel\Lrrk

Associated Insertion(s)

Carried in Construct

P{UAS-Lrrk.3KD}

Also Known As

UAS-dLRRK-3KD, 3KD

Key Links

Transgenic product class

missense_variant

(Imai et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Imai et al., 2008)

Mutagen

in vitro construct

(Imai et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Lrrk.3KD}

Cytology

Description

UAS regulatory sequences drive expression of a kinase-dead form of Lrrk.

(Imai et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Lrrk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 8

CEC

(Inoshita et al., 2022)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^109(2)80

(Lin et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^ppk.PU

(Lin et al., 2015)

Phenotype Manifest In

dendrite, with Scer\GAL4^109(2)80

(Lin et al., 2015)

dendrite, with Scer\GAL4^ppk.PU

(Lin et al., 2015)

dendritic arborizing neuron, with Scer\GAL4^109(2)80

(Lin et al., 2015)

dendritic arborizing neuron, with Scer\GAL4^ppk.PU

(Lin et al., 2015)

Golgi stack, with Scer\GAL4^ppk.PU

(Lin et al., 2015)

Detailed Description

Statement

Reference

Expression of Lrrk^3KD.Scer\UAS under the control of Scer\GAL4^Mhc.PW does not result in any significant difference in amplitude of miniature excitatory junction currents (mEJCs), EJCs, or quantal content of larval neuromuscular junctions, as compared to controls.

(Penney et al., 2016)

The dendrites of dendritic arborizing neurons expressing Lrrk^3KD.Scer\UAS under the control of Scer\GAL4^ppk.PU exhibit a reduced proportion of stationary golgi outposts (GOPs) compared with controls. The dynamic GOPs that move in the anterograde direction (towards dendritic ends) show increase displacement and increased velocity. Retrograde displacement (towards cell bodies) and velocity are unaffected. The distribution of GOPs is shifted towards the distal region.

Expression of Lrrk^3KD.Scer\UAS under the control of Scer\GAL4^109(2)80 induces slight dendritic overgrowth compared with controls, mainly affecting higher order dendrites. Expressing Lrrk^3KD.Scer\UAS specifically in Class IV da neurons under the control of Scer\GAL4^ppk.PU also induces dendritic overgrowth.

(Lin et al., 2015)

Flies expressing Lrrk^3KD.Scer\UAS under the control of Scer\GAL4^ple.PG do not show a progressive reduction in electroretinogram amplitude (there is no significant difference in the amplitude seen in 3 and 28 day old flies).

(Hindle et al., 2013)

Pre-synaptic or post-synaptic expression of Lrrk^3KD.Scer\UAS (under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Mhc.PW) does not significantly affect the number of type I boutons compared to controls.

(Lee et al., 2010)

The number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster and the number of neurons in the protocerebral posterior medial 3 cluster is normal in 60 day old adults expressing Lrrk^{Y1383C.Scer\UAS} under the control of Scer\GAL4^ple.PF.

(Imai et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Suppressor of

Statement

Reference

Lrrk^3KD.UAS, Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

NOT Suppressor of

Statement

Reference

Lrrk^3KD.UAS, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa

(Kanao et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is a suppressor of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is a suppressor of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Scer\GAL4^nSyb.PP/Lrrk^3KD.UAS is a suppressor of medulla intrinsic columnar neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^3KD.UAS, Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

NOT Suppressor of

Statement

Reference

Lrrk^3KD.UAS, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa

(Kanao et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa is only slightly suppressed (or not at all) by co-expression of Lrrk^3KD.Scer\UAS.

(Ruan et al., 2016)

Co-expression of foxo^Scer\UAS.cPa and for^P1.Scer\UAS under the control of Scer\GAL4^GMR.PF results in degeneration of the eye. The severity of this phenotype is not suppressed by co-expression of Lrrk^3KD.Scer\UAS.

(Kanao et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^Mhc.PW/Lrrk^3KD.UAS fails to rescue Lrrk^e03680

(Lee et al., 2010)

Scer\GAL4^elav-C155/Lrrk^3KD.UAS fails to rescue Lrrk^e03680

(Lee et al., 2010)

Comments

Muscle or neuronal expression of Lrrk^3KD.Scer\UAS (under the control of Scer\GAL4^Mhc.PW or Scer\GAL4^elav-C155 respectively) fails to rescue the synaptic morphology phenotype and average bouton size found in Lrrk^e03680 homozygous mutants.

(Lee et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Lrrk^3KD.Scer\UAS

Lrrk^3KD.UAS

dLRRK^3KD

(Ruan et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (11)

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