FlyBase Allele Report: Dmel\Drp1[UAS.cDb]

General Information

Symbol

Dmel\Drp1^UAS.cDb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct b of DuBoff

FlyBase ID

FBal0278275

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

Carried in Construct

P{UAS-Drp1.Db}

Also Known As

UAS-DRP1

Key Links

Transgenic product class

cDNA

(Duboff et al., 2012)

wild_type

(Duboff et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Duboff et al., 2012)

wild_type

(Duboff et al., 2012)

Mutagen

in vitro construct

(Duboff et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Drp1.Db}

Cytology

Description

UAS regulatory sequences drive expression of a Drp1 cDNA (AT04516).

(Duboff et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Drp1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates hereditary spastic paraplegia

modeled by Arl6IP1^GD3027

(Fowler and O'Sullivan, 2016)

modeled by Arl6IP1^KK109154

(Fowler and O'Sullivan, 2016)

modeled by Rtnl1^GD15707

(Fowler and O'Sullivan, 2016)

ameliorates synucleinopathy

modeled by Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

ameliorates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Duboff et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^VGlut1-OK371

(Smith et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Drp1^UAS.cDb under the control of Scer\GAL4^VGlut-OK371 significantly increases mitochondrial number in axons of adult neurons, compared to controls, with no effect on mitochondrial length.

(Smith et al., 2019)

The expression of Drp1^Scer\UAS.cDb under the control of Scer\GAL4^elav-C155 has no significant effect on adult locomotion, or on either the number of neurons or the mitochondria size in the adult brain, as compared to controls.

(Ordonez et al., 2018)

Expression of Drp1^Scer\UAS.cDb under the control of Scer\GAL4^RapGAP1-OK6 results in increased circularity of mitochondria (due to increased mitochondrial fission) within distal portions of anterior (shorter, synapsing at segment 3) motor neuron axons in third instar larvae, but has no significant effect on the mitochondrial morphology in the the distal portions of the longer posterior (synapsing at segment 7) motoneuron axons. Expression under the Scer\GAL4^da.G32 driver leads to disrupted smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal boutons at the neuromuscular junctions of both anterior and posterior motor neurons.

Expression of Drp1^Scer\UAS.cDb using the Scer\GAL4^nSyb.PS driver does not significantly alter climbing ability of the adult flies compared to controls.

(Fowler and O'Sullivan, 2016)

Expression of Drp1^Scer\UAS.cDb under the control of Scer\GAL4^elav.PU has no effect on mitochondrial length in adult neurons. The amount of cell death and cell cycle activation are similar to controls, as are levels of superoxide production.

(Duboff et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Drp1^UAS.cDb, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

NOT suppressed by

Statement

Reference

Drp1^UAS.cDb, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Suppressor of

Statement

Reference

Drp1^UAS.cDb, Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | adult stage phenotype of Arl6IP1^GD3027, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | adult stage phenotype of Arl6IP1^KK109154, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Scer\GAL4^VGlut1-OK371/Drp1^UAS.cDb is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^elav-C155/Drp1^UAS.cDb is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Scer\GAL4^elav-C155/Drp1^UAS.cDb is a suppressor of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Drp1^UAS.cDb, Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Arl6IP1^GD3027, Scer\GAL4^nSyb.PS

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Arl6IP1^KK109154, Scer\GAL4^nSyb.PS

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^nSyb.PS is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Rtnl1^GD15707, Scer\GAL4^nSyb.PS

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^elav.PU is a suppressor of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Drp1^UAS.cDb, Scer\GAL4^elav.PU is a suppressor of abnormal cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Drp1^UAS.cDb, Scer\GAL4^elav.PU is a suppressor of abnormal cell cycle phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Drp1^UAS.cDb, Scer\GAL4^VGlut1-OK371 has mitochondrion | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Drp1^UAS.cDb, Scer\GAL4^VGlut1-OK371 has axon | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

NOT suppressed by

Statement

Reference

Drp1^UAS.cDb, Scer\GAL4^VGlut1-OK371 has mitochondrion | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Drp1^UAS.cDb, Scer\GAL4^VGlut1-OK371 has axon | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Suppressor of

Statement

Reference

Drp1^UAS.cDb, Scer\GAL4^da.G32 is a suppressor of embryonic/larval epidermis | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^da.G32

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^da.G32 is a suppressor of mitochondrion | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^da.G32

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of embryonic/larval motor neuron | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of mitochondrion | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of mitochondrion | larval stage | decreased number phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of axon | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of embryonic/larval motor neuron phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of mitochondrion | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of embryonic/larval motor neuron | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of mitochondrion | larval stage | decreased number phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of axon | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Scer\GAL4^VGlut1-OK371/Drp1^UAS.cDb is a suppressor of mitochondrion | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^VGlut1-OK371/Drp1^UAS.cDb is a suppressor of axon | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^elav-C155/Drp1^UAS.cDb is a suppressor of adult brain phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Scer\GAL4^elav-C155/Drp1^UAS.cDb is a suppressor of neuron | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Scer\GAL4^elav-C155/Drp1^UAS.cDb is a suppressor of dopaminergic medulla neuron phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Scer\GAL4^elav-C155/Drp1^UAS.cDb is a suppressor of mitochondrion | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of motor neuron | third instar larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of mitochondrion | third instar larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of axon | third instar larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of motor neuron | third instar larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of mitochondrion | third instar larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of axon | third instar larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of motor neuron | third instar larval stage phenotype of Rtnl1^GD15707, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of axon | third instar larval stage phenotype of Rtnl1^GD15707, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6 is a suppressor of mitochondrion | third instar larval stage phenotype of Rtnl1^GD15707, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Drp1^UAS.cDb, Scer\GAL4^elav.PU is a suppressor of adult brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Drp1^UAS.cDb, Scer\GAL4^elav.PU is a suppressor of mitochondrion phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of either Arl6IP1^GD3027 or Arl6IP1^KK109154 RNAi leads to abnormal elongated mitochondrial morphology in the distal portions of the longer posterior motor neuron axons (synapsing at segment 7) in third instar larvae (with the Scer\GAL4^RapGAP1-OK6 driver) as well as age-progressive climbing ability defects in adults (with the Scer\GAL4^nSyb.PS driver) and both these phenotypes are significantly suppressed by co-expression of Drp1^Scer\UAS.cDb (without compromising adult lifespan).

However, the disruption of the smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal synaptic boutons at the neuromuscular junctions of both anterior and posterior motor neurons observed in third instar larvae expressing Arl6IP1^GD3027 or Arl6IP1^KK109154 or Drp1^Scer\UAS.cDb alone under the control of Scer\GAL4^da.G32 is not significantly changed when Drp1^Scer\UAS.cDb is co-expressed with either of the Arl6IP1 RNAi lines.

The abnormal elongated morphology of mitochondria in the distal portions of long posterior (synapsing at segment 7) motor neurons axons observed in third instar larvae expressing Rtnl1^GD15707 (under the Scer\GAL4^RapGAP1-OK6 driver) as well the rapid age-progressive decline in climbing abilities in Rtnl1^GD15707-expressing adults (using the Scer\GAL4^nSyb.PS driver) is rescued by co-expression of Drp1^Scer\UAS.cDb (without compromising adult lifespan).

(Fowler and O'Sullivan, 2016)

Xenogenetic Interactions

Statement

Reference

The additional expression of Drp1^Scer\UAS.cDbunder the control of Scer\GAL4^elav-C155 partially suppresses the adult locomotor activity defects, the adult brain vacuolization, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased mitochondria size in the adult brain resulting from the expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb.

(Ordonez et al., 2018)

Expression of Drp1^Scer\UAS.cDb significantly suppresses the increase in mitochondria length seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU. The increases in neurotoxicity and cell cycle activation are also significantly rescued.

Expression of Drp1^Scer\UAS.cDb suppresses the increase in superoxide production seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU.

(Duboff et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Drp1^Scer\UAS.cDb

Drp1^UAS.cDb

Name Synonyms

Saccharomyces cerevisiae UAS construct b of DuBoff

Secondary FlyBase IDs

References (6)

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