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General Information
Symbol
Dmel\Drp1UAS.cDb
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct b of DuBoff
FlyBase ID
FBal0278275
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DRP1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a Drp1 cDNA (AT04516).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Drp1UAS.cDb under the control of Scer\GAL4VGlut-OK371 significantly increases mitochondrial number in axons of adult neurons, compared to controls, with no effect on mitochondrial length.

The expression of Drp1Scer\UAS.cDb under the control of Scer\GAL4elav-C155 has no significant effect on adult locomotion, or on either the number of neurons or the mitochondria size in the adult brain, as compared to controls.

Expression of Drp1Scer\UAS.cDb under the control of Scer\GAL4RapGAP1-OK6 results in increased circularity of mitochondria (due to increased mitochondrial fission) within distal portions of anterior (shorter, synapsing at segment 3) motor neuron axons in third instar larvae, but has no significant effect on the mitochondrial morphology in the the distal portions of the longer posterior (synapsing at segment 7) motoneuron axons. Expression under the Scer\GAL4da.G32 driver leads to disrupted smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal boutons at the neuromuscular junctions of both anterior and posterior motor neurons.

Expression of Drp1Scer\UAS.cDb using the Scer\GAL4nSyb.PS driver does not significantly alter climbing ability of the adult flies compared to controls.

Expression of Drp1Scer\UAS.cDb under the control of Scer\GAL4elav.PU has no effect on mitochondrial length in adult neurons. The amount of cell death and cell cycle activation are similar to controls, as are levels of superoxide production.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
NOT suppressed by
Suppressor of
Statement
Reference
Phenotype Manifest In
NOT Enhanced by
NOT suppressed by
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of either Arl6IP1GD3027 or Arl6IP1KK109154 RNAi leads to abnormal elongated mitochondrial morphology in the distal portions of the longer posterior motor neuron axons (synapsing at segment 7) in third instar larvae (with the Scer\GAL4RapGAP1-OK6 driver) as well as age-progressive climbing ability defects in adults (with the Scer\GAL4nSyb.PS driver) and both these phenotypes are significantly suppressed by co-expression of Drp1Scer\UAS.cDb (without compromising adult lifespan).

However, the disruption of the smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal synaptic boutons at the neuromuscular junctions of both anterior and posterior motor neurons observed in third instar larvae expressing Arl6IP1GD3027 or Arl6IP1KK109154 or Drp1Scer\UAS.cDb alone under the control of Scer\GAL4da.G32 is not significantly changed when Drp1Scer\UAS.cDb is co-expressed with either of the Arl6IP1 RNAi lines.

The abnormal elongated morphology of mitochondria in the distal portions of long posterior (synapsing at segment 7) motor neurons axons observed in third instar larvae expressing Rtnl1GD15707 (under the Scer\GAL4RapGAP1-OK6 driver) as well the rapid age-progressive decline in climbing abilities in Rtnl1GD15707-expressing adults (using the Scer\GAL4nSyb.PS driver) is rescued by co-expression of Drp1Scer\UAS.cDb (without compromising adult lifespan).

Xenogenetic Interactions
Statement
Reference

The additional expression of Drp1Scer\UAS.cDbunder the control of Scer\GAL4elav-C155 partially suppresses the adult locomotor activity defects, the adult brain vacuolization, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased mitochondria size in the adult brain resulting from the expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb.

Expression of Drp1Scer\UAS.cDb significantly suppresses the increase in mitochondria length seen when Hsap\MAPTR406W.Scer\UAS is expressed under the control of Scer\GAL4elav.PU. The increases in neurotoxicity and cell cycle activation are also significantly rescued.

Expression of Drp1Scer\UAS.cDb suppresses the increase in superoxide production seen when Hsap\MAPTR406W.Scer\UAS is expressed under the control of Scer\GAL4elav.PU.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Drp1Scer\UAS.cDb
Drp1UAS.cDb
Name Synonyms
Saccharomyces cerevisiae UAS construct b of DuBoff
Secondary FlyBase IDs
    References (5)