Expression of either Arl6IP1GD3027 or Arl6IP1KK109154 RNAi leads to abnormal elongated mitochondrial morphology in the distal portions of the longer posterior motor neuron axons (synapsing at segment 7) in third instar larvae (with the Scer\GAL4RapGAP1-OK6 driver) as well as age-progressive climbing ability defects in adults (with the Scer\GAL4nSyb.PS driver) and both these phenotypes are significantly suppressed by co-expression of Drp1Scer\UAS.cDb (without compromising adult lifespan).
However, the disruption of the smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal synaptic boutons at the neuromuscular junctions of both anterior and posterior motor neurons observed in third instar larvae expressing Arl6IP1GD3027 or Arl6IP1KK109154 or Drp1Scer\UAS.cDb alone under the control of Scer\GAL4da.G32 is not significantly changed when Drp1Scer\UAS.cDb is co-expressed with either of the Arl6IP1 RNAi lines.
The abnormal elongated morphology of mitochondria in the distal portions of long posterior (synapsing at segment 7) motor neurons axons observed in third instar larvae expressing Rtnl1GD15707 (under the Scer\GAL4RapGAP1-OK6 driver) as well the rapid age-progressive decline in climbing abilities in Rtnl1GD15707-expressing adults (using the Scer\GAL4nSyb.PS driver) is rescued by co-expression of Drp1Scer\UAS.cDb (without compromising adult lifespan).
The additional expression of Drp1Scer\UAS.cDbunder the control of Scer\GAL4elav-C155 partially suppresses the adult locomotor activity defects, the adult brain vacuolization, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased mitochondria size in the adult brain resulting from the expression of Hsap\SNCANcra\QUAS.cOa under the control of Ncra\QFQF2.nSyb.
Expression of Drp1Scer\UAS.cDb significantly suppresses the increase in mitochondria length seen when Hsap\MAPTR406W.Scer\UAS is expressed under the control of Scer\GAL4elav.PU. The increases in neurotoxicity and cell cycle activation are also significantly rescued.