FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Arl6IP1KK109154
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General Information
Symbol
Dmel\Arl6IP1KK109154
Species
D. melanogaster
Name
FlyBase ID
FBal0236094
Feature type
allele
Associated gene
Dmel\Arl6IP1
Associated Insertion(s)
Carried in Construct
P{KK109154}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK109154}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK109154
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  hereditary spastic paraplegia
      is ameliorated by Drp1UAS.cDb
      (Fowler and O'Sullivan, 2016)
      model of  hereditary spastic paraplegia 61
      is exacerbated by Drp1K38A.UAS.Tag:HA
      (Fowler et al., 2020)
      is ameliorated by Drp1UAS.Tag:HA
      (Fowler et al., 2020)
      is exacerbated by Orp8HMS01223
      (Byrne et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal segment motor neuron, with Scer\GAL4nSyb.PS

      (Byrne et al., 2022)

      abdominal segment motor neuron, with Scer\GAL4RapGAP1-OK6

      (Byrne et al., 2022)

      axon, with Scer\GAL4nSyb.PS

      (Byrne et al., 2022)

      axon, with Scer\GAL4RapGAP1-OK6

      (Byrne et al., 2022)

      axon | larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      axon | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler and O'Sullivan, 2016)

      embryonic/larval motor neuron | larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      larval abdominal segment 7, with Scer\GAL4nSyb.PS

      (Byrne et al., 2022)

      larval abdominal segment 7, with Scer\GAL4RapGAP1-OK6

      (Byrne et al., 2022)

      lipid droplet | increased number | third instar larval stage, with Scer\GAL4nSyb.PS

      (Byrne et al., 2022)

      lipid droplet | increased number | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Byrne et al., 2022)

      mitochondrion | decreased number | larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      mitochondrion | larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      mitochondrion | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler and O'Sullivan, 2016)

      motor neuron | third instar larval stage, with Scer\GAL4da.G32

      (Fowler and O'Sullivan, 2016)

      motor neuron | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Fowler and O'Sullivan, 2016)

      NMJ bouton | third instar larval stage, with Scer\GAL4da.G32

      (Fowler and O'Sullivan, 2016)

      smooth endoplasmic reticulum | third instar larval stage, with Scer\GAL4da.G32

      (Fowler and O'Sullivan, 2016)
      Detailed Description
      Statement
      Reference

      The expression of Arl6IP1KK109154 under the control of Scer\GAL4nSyb.PS leads to adults that show a progressive decrease in climbing ability. In third instar larvae, axon bundles of long motor neurons (i.e. targeting A7) display an increased number of lipid droplets.

      (Byrne et al., 2022)

      Larval motor neurons expressing Arl6IP1KK109154 under the control of Scer\GAL4RapGAP1-OK6 show longer mitochondria in posterior axon bundles and reduced mitochondrial load in terminal boutons within posterior neuromuscular junctions.

      Larvae and adults expressing Arl6IP1GD3027 under the control of Scer\GAL4nSyb.PS show decreased crawling and climbing activity, respectively.

      (Fowler et al., 2020)

      Expression of Arl6IP1KK109154 under the control of Scer\GAL4nSyb.PS results in a more rapid age-progressive decline in climbing abilities of adult flies as well as defects in larval crawling without significantly affecting their adult lifespan. Expression under the Scer\GAL4da.G32 driver does not significantly change the number or the type composition of synaptic boutons on neuromuscular junctions (NMJs) in third instar larval muscles, but disrupts the smooth endoplasmic reticulum network morphology (assessed by a marker expression pattern) in the terminal synaptic boutons at the ends of both shorter anterior motor neuron axons (synapsing at segment 3) and longer posterior axons (synapsing at segment 7).

      Scer\GAL4RapGAP1-OK6-controlled expression does not lead to any gross defects in axonal transport, but mitochondria in the distal portions of longer posterior axons are significantly elongated compared to controls, whereas the mitochondrial morphology in the distal portions of shorter anterior axons is normal.

      (Fowler and O'Sullivan, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has embryonic/larval motor neuron phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has mitochondrion | larval stage phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has embryonic/larval motor neuron | larval stage phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has mitochondrion | larval stage | decreased number phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has axon | larval stage phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler et al., 2020)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has motor neuron | third instar larval stage phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler and O'Sullivan, 2016)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has mitochondrion | third instar larval stage phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler and O'Sullivan, 2016)

      Arl6IP1KK109154, Scer\GAL4RapGAP1-OK6 has axon | third instar larval stage phenotype, suppressible by Drp1UAS.cDb, Scer\GAL4RapGAP1-OK6

      (Fowler and O'Sullivan, 2016)
      NOT suppressed by
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Arl6IP1KK109154 leads to abnormal elongated mitochondrial morphology in the distal portions of the longer posterior motor neuron axons (synapsing at segment 7) in third instar larvae (with the Scer\GAL4RapGAP1-OK6 driver) as well as age-progressive climbing ability defects in adults (with the Scer\GAL4nSyb.PS driver) and both these phenotypes are significantly suppressed by co-expression of Drp1Scer\UAS.cDb (without compromising adult lifespan).

      However, the disruption of the smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal synaptic boutons at the neuromuscular junctions of both anterior and posterior motor neurons observed in third instar larvae expressing either Arl6IP1KK109154 or Drp1Scer\UAS.cDb alone under the control of Scer\GAL4da.G32 is not significantly changed when the two transgenes are co-expressed.

      (Fowler and O'Sullivan, 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Arl6IP1KK109154
      CG10326KK109154
      Name Synonyms
      Secondary FlyBase IDs
        References (4)