FlyBase Allele Report: Dmel\Drp1[K38A.UAS.Tag:HA]

General Information

Symbol

Dmel\Drp1^{K38A.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0230286

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

Carried in Construct

P{UAS-Drp1.K38A.HA}

Also Known As

UAS-drp1^K38A

Key Links

Transgenic product class

dominant_negative_variant

(Park et al., 2009)

missense_variant

(Park et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Park et al., 2009)

missense_variant

(Park et al., 2009)

Mutagen

in vitro construct

(Park et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Drp1.K38A.HA}

Cytology

Description

UAS regulatory sequences drive expression of a dominant-negative form of Drp1 which is tagged at the C-terminal end with Tag:HA.

(Park et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Drp1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT exacerbate Charcot-Marie-Tooth disease type 2

modeled by Marf^R94Qlike.UAS

(El Fissi et al., 2018)

exacerbates Charcot-Marie-Tooth disease type 2

modeled by Marf^{R364Wlike.UAS}

(El Fissi et al., 2018)

exacerbates hereditary spastic paraplegia 61

modeled by Arl6IP1^KK109154

(Fowler et al., 2020)

modeled by Arl6IP1^GD3027

(Fowler et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | larval stage, with Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

chemical resistant | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

Phenotype Manifest In

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

embryonic/larval motor neuron | larval stage, with Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

mitochondrion | larval stage, with Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

NMJ bouton | larval stage, with Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Detailed Description

Statement

Reference

Expression of Drp1^{K38A.UAS.Tag:HA} under the control of Scer\GAL4^VGlut-OK371 induces bigger mitochondria in larval motor neuron cell bodies and loss of mitochondria in their neuromuscular boutons, as compared to controls.

(El Fissi et al., 2018)

Expression of Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ple.PF leads to protection from rotenone toxicity, with mutant flies displaying less PPL1 dopaminergic neuron loss in adult brains after rotenone feeding, in contrast to controls.

(Hwang et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut1-OK371 has abnormal size | larval stage phenotype, suppressible by Marf^RNAi.UAS.cUa, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is an enhancer of abnormal size | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^hs.PB/Drp1^{K38A.UAS.Tag:HA} is an enhancer of visible phenotype of Pink1^B9

(Park et al., 2009)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is a non-enhancer of abnormal size | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Suppressor of

Statement

Reference

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

NOT Suppressor of

Statement

Reference

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^nSyb.PS is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^nSyb.PS is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Arl6IP1^GD3027, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^nSyb.PS is a non-suppressor of abnormal locomotor behavior | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^nSyb.PS is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Arl6IP1^KK109154, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is a non-suppressor of abnormal size | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{WT.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color | adult stage phenotype of Hsap\FUS^{WT.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{P525L.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color | adult stage phenotype of Hsap\FUS^{P525L.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Scer\GAL4^hs.PB/Drp1^{K38A.UAS.Tag:HA} is a non-suppressor of flightless phenotype of Pink1^B9

(Park et al., 2009)

Other

Statement

Reference

Arl6IP1^KK109154, Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^nSyb.PS has short lived phenotype

(Fowler et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut1-OK371 has mitochondrion | larval stage phenotype, suppressible by Marf^RNAi.UAS.cUa, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut1-OK371 has embryonic/larval motor neuron | larval stage phenotype, suppressible by Marf^RNAi.UAS.cUa, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut1-OK371 has mitochondrion | larval stage phenotype, suppressible by Marf^WT.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut1-OK371 has embryonic/larval motor neuron | larval stage phenotype, suppressible by Marf^WT.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut1-OK371 has NMJ bouton | larval stage phenotype, suppressible by Marf^WT.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut1-OK371 has NMJ bouton | larval stage phenotype, suppressible by Marf^RNAi.UAS.cUa, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Enhancer of

Statement

Reference

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is an enhancer of axon | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is an enhancer of embryonic/larval motor neuron | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is an enhancer of axon | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is an enhancer of embryonic/larval motor neuron | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is an enhancer of mitochondrion | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is an enhancer of mitochondrion | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is an enhancer of mitochondrion | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is an enhancer of embryonic/larval motor neuron | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^hs.PB/Drp1^{K38A.UAS.Tag:HA} is an enhancer of wing phenotype of Pink1^B9

(Park et al., 2009)

Scer\GAL4^hs.PB/Drp1^{K38A.UAS.Tag:HA} is an enhancer of adult thorax phenotype of Pink1^B9

(Park et al., 2009)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is a non-enhancer of mitochondrion | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is a non-enhancer of embryonic/larval motor neuron | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Suppressor of

Statement

Reference

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^insc-Mz1407 is a suppressor of type II neuroblast | increased number phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

NOT Suppressor of

Statement

Reference

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is a non-suppressor of mitochondrion | larval stage | decreased number phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6 is a non-suppressor of mitochondrion | larval stage | decreased number phenotype of Arl6IP1^KK109154, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is a non-suppressor of mitochondrion | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^{K38A.UAS.Tag:HA} is a non-suppressor of embryonic/larval motor neuron | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of eye | adult stage phenotype of Hsap\FUS^{WT.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of eye | adult stage phenotype of Hsap\FUS^{P525L.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of ommatidium | adult stage phenotype of Hsap\FUS^{P525L.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^GMR.PU is a non-suppressor of interommatidial bristle phenotype of Hsap\FUS^{P525L.UAS.RFP(Unk),Tag:HA}, Scer\GAL4^GMR.PU

(Deng et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Drp1^{K38A.Scer\UAS.T:Ivir\HA1} suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

(Lee et al., 2013)

The downturned wing and crushed thorax phenotypes of Pink1^B9 mutant flies are enhanced by expression of Drp1^{K38A.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.PB.

(Park et al., 2009)

Xenogenetic Interactions

Statement

Reference

The adult eye phenotype (rough surface, pigmentation loss) characteristic for flies expressing either Hsap\FUS^{WT.Scer\UAS.T:Disc\RFP,T:Ivir\HA1} or Hsap\FUS^{P525L.Scer\UAS.T:Disc\RFP,T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU is not significantly modified by co-expression of Drp1^{K38A.Scer\UAS.T:Ivir\HA1}.

(Deng et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

95247

w^*; P{UAS-Drp1.K38A.HA}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Drp1^DN

(Liu and Lu, 2010)

Drp1^{K38A.Scer\UAS.T:Ivir\HA1}

Drp1^{K38A.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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