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General Information
Symbol
Dmel\Arl6IP1GD3027
Species
D. melanogaster
Name
FlyBase ID
FBal0198984
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In the epidermis of larvae expressing Arl6IP1GD3027 under the control of Scer\GAL4da.G32 mitochondria are longer and an increased proportion are apposed to the ER.

Larval motor neurons expressing Arl6IP1GD3027 under the control of Scer\GAL4RapGAP1-OK6 show longer mitochondria in posterior axon bundles and reduced mitochondrial load in terminal boutons within posterior neuromuscular junctions.

Larvae and adults expressing Arl6IP1GD3027 under the control of Scer\GAL4nSyb.PS show decreased crawling and climbing activity, respectively.

Expression of Arl6IP1GD3027 under the control of Scer\GAL4nSyb.PS results in a more rapid age-progressive decline in climbing abilities of adult flies as well as defects in larval crawling without significantly affecting their adult lifespan. Expression under the Scer\GAL4da.G32 driver does not significantly change the number or the type composition of synaptic boutons on neuromuscular junctions (NMJs) in third instar larval muscles, but disrupts the smooth endoplasmic reticulum network morphology (assessed by a marker expression pattern) in the terminal synaptic boutons at the ends of both shorter anterior motor neuron axons (synapsing at segment 3) and longer posterior axons (synapsing at segment 7).

Scer\GAL4RapGAP1-OK6-controlled expression does not lead to any gross defects in axonal transport, but mitochondria in the distal portions of longer posterior axons are significantly elongated compared to controls, whereas the mitochondrial morphology in the distal portions of shorter anterior axons is normal.

Adults expressing CG10326GD3027 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
NOT Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Arl6IP1GD3027 leads to abnormal elongated mitochondrial morphology in the distal portions of the longer posterior motor neuron axons (synapsing at segment 7) in third instar larvae (with the Scer\GAL4RapGAP1-OK6 driver) as well as age-progressive climbing ability defects in adults (with the Scer\GAL4nSyb.PS driver) and both these phenotypes are significantly suppressed by co-expression of Drp1Scer\UAS.cDb (without compromising adult lifespan).

However, the disruption of the smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal synaptic boutons at the neuromuscular junctions of both anterior and posterior motor neurons observed in third instar larvae expressing either Arl6IP1GD3027 or Drp1Scer\UAS.cDb alone under the control of Scer\GAL4da.G32 is not significantly changed when the two transgenes are co-expressed.

The rapid age-dependent loss of climbing abilities and reduced lifespan characteristic for adult flies expressing MarfGD11094 under the control of Scer\GAL4nSyb.PS is exacerbated by co-expression of Arl6IP1GD3027. However, the increase in mitochondrial circularity induced by Scer\GAL4RapGAP1-OK6-driven expression of MarfGD11094 in the both anterior (synapsing to segment 3) and posterior (synapsing to segment 7) motor neuron axons in third instar larvae is not greatly affected by co-expression with Arl6IP1GD3027, which on its own induces mitochondrial elongation in the posterior axons.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Arl6IP1GD3027
CG10326GD3027
Name Synonyms
Secondary FlyBase IDs
    References (7)