FlyBase Allele Report: Dmel\Arl6IP1[GD3027]

General Information

Symbol

Dmel\Arl6IP1^GD3027

Species

D. melanogaster

Name

FlyBase ID

FBal0198984

Feature type

allele

Associated gene

Dmel\Arl6IP1

Associated Insertion(s)

Carried in Construct

P{GD3027}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3027}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3027

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of hereditary spastic paraplegia

CEA

(Fowler and O'Sullivan, 2016)

model of hereditary spastic paraplegia 61

CEA

(Fowler et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of hereditary spastic paraplegia

is ameliorated by Drp1^UAS.cDb

(Fowler and O'Sullivan, 2016)

model of hereditary spastic paraplegia 61

is exacerbated by Drp1^{K38A.UAS.Tag:HA}

(Fowler et al., 2020)

is ameliorated by Drp1^UAS.Tag:HA

(Fowler et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

axon | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

embryonic/larval epidermis | larval stage, with Scer\GAL4^da.G32

(Fowler et al., 2020)

embryonic/larval motor neuron | larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

mitochondrion | decreased number | larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

mitochondrion | larval stage, with Scer\GAL4^da.G32

(Fowler et al., 2020)

mitochondrion | larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

mitochondrion | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

motor neuron | third instar larval stage, with Scer\GAL4^da.G32

(Fowler and O'Sullivan, 2016)

motor neuron | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

NMJ bouton | third instar larval stage, with Scer\GAL4^da.G32

(Fowler and O'Sullivan, 2016)

smooth endoplasmic reticulum | third instar larval stage, with Scer\GAL4^da.G32

(Fowler and O'Sullivan, 2016)

Detailed Description

Statement

Reference

In the epidermis of larvae expressing Arl6IP1^GD3027 under the control of Scer\GAL4^da.G32 mitochondria are longer and an increased proportion are apposed to the ER.

Larval motor neurons expressing Arl6IP1^GD3027 under the control of Scer\GAL4^RapGAP1-OK6 show longer mitochondria in posterior axon bundles and reduced mitochondrial load in terminal boutons within posterior neuromuscular junctions.

Larvae and adults expressing Arl6IP1^GD3027 under the control of Scer\GAL4^nSyb.PS show decreased crawling and climbing activity, respectively.

(Fowler et al., 2020)

Expression of Arl6IP1^GD3027 under the control of Scer\GAL4^nSyb.PS results in a more rapid age-progressive decline in climbing abilities of adult flies as well as defects in larval crawling without significantly affecting their adult lifespan. Expression under the Scer\GAL4^da.G32 driver does not significantly change the number or the type composition of synaptic boutons on neuromuscular junctions (NMJs) in third instar larval muscles, but disrupts the smooth endoplasmic reticulum network morphology (assessed by a marker expression pattern) in the terminal synaptic boutons at the ends of both shorter anterior motor neuron axons (synapsing at segment 3) and longer posterior axons (synapsing at segment 7).

Scer\GAL4^RapGAP1-OK6-controlled expression does not lead to any gross defects in axonal transport, but mitochondria in the distal portions of longer posterior axons are significantly elongated compared to controls, whereas the mitochondrial morphology in the distal portions of shorter anterior axons is normal.

(Fowler and O'Sullivan, 2016)

Adults expressing CG10326^GD3027 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3027

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Arl6IP1^GD3027, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | adult stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^nSyb.PS

(Fowler and O'Sullivan, 2016)

NOT suppressed by

Statement

Reference

Arl6IP1^GD3027, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | larval stage phenotype, non-suppressible by Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^nSyb.PS

(Fowler et al., 2020)

Enhancer of

Statement

Reference

Arl6IP1^GD3027, Scer\GAL4^nSyb.PS is an enhancer of abnormal locomotor behavior | adult stage phenotype of Marf^GD11094, Scer\GAL4^nSyb.PS

(Fowler and O'Sullivan, 2016)

Arl6IP1^GD3027, Scer\GAL4^nSyb.PS is an enhancer of short lived phenotype of Marf^GD11094, Scer\GAL4^nSyb.PS

(Fowler and O'Sullivan, 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has mitochondrion | larval stage phenotype, enhanceable by Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has axon | larval stage phenotype, enhanceable by Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has embryonic/larval motor neuron | larval stage phenotype, enhanceable by Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Suppressed by

Statement

Reference

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has embryonic/larval motor neuron | larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has mitochondrion | larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has mitochondrion | larval stage | decreased number phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has axon | larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^da.G32 has embryonic/larval epidermis | larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^da.G32

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^da.G32 has mitochondrion | larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^da.G32

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has mitochondrion | third instar larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has axon | third instar larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has motor neuron | third instar larval stage phenotype, suppressible by Drp1^UAS.cDb, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

NOT suppressed by

Statement

Reference

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has mitochondrion | larval stage | decreased number phenotype, non-suppressible by Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^RapGAP1-OK6

(Fowler et al., 2020)

NOT Enhancer of

Statement

Reference

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 is a non-enhancer of motor neuron | third instar larval stage phenotype of Marf^GD11094, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 is a non-enhancer of axon | third instar larval stage phenotype of Marf^GD11094, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Arl6IP1^GD3027, Scer\GAL4^RapGAP1-OK6 is a non-enhancer of mitochondrion | third instar larval stage phenotype of Marf^GD11094, Scer\GAL4^RapGAP1-OK6

(Fowler and O'Sullivan, 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Arl6IP1^GD3027 leads to abnormal elongated mitochondrial morphology in the distal portions of the longer posterior motor neuron axons (synapsing at segment 7) in third instar larvae (with the Scer\GAL4^RapGAP1-OK6 driver) as well as age-progressive climbing ability defects in adults (with the Scer\GAL4^nSyb.PS driver) and both these phenotypes are significantly suppressed by co-expression of Drp1^Scer\UAS.cDb (without compromising adult lifespan).

However, the disruption of the smooth endoplasmic reticulum network (assessed by a marker expression pattern) in the terminal synaptic boutons at the neuromuscular junctions of both anterior and posterior motor neurons observed in third instar larvae expressing either Arl6IP1^GD3027 or Drp1^Scer\UAS.cDb alone under the control of Scer\GAL4^da.G32 is not significantly changed when the two transgenes are co-expressed.

The rapid age-dependent loss of climbing abilities and reduced lifespan characteristic for adult flies expressing Marf^GD11094 under the control of Scer\GAL4^nSyb.PS is exacerbated by co-expression of Arl6IP1^GD3027. However, the increase in mitochondrial circularity induced by Scer\GAL4^RapGAP1-OK6-driven expression of Marf^GD11094 in the both anterior (synapsing to segment 3) and posterior (synapsing to segment 7) motor neuron axons in third instar larvae is not greatly affected by co-expression with Arl6IP1^GD3027, which on its own induces mitochondrial elongation in the posterior axons.

(Fowler and O'Sullivan, 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3027

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Arl6IP1^GD3027

CG10326^GD3027

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close