FlyBase Gene Report: Dmel\nSyb

adult brain cell body rind

photoreceptor neuron

(DiAntonio et al., 1993)

• neuronal cell body

(DiAntonio et al., 1993)

cell body of adult optic lobe

(DiAntonio et al., 1993)

adult ventral nerve cord

• neuronal cell body

(DiAntonio et al., 1993)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(DiAntonio et al., 1993)

Additional Descriptive Data

n-syb transcripts are first detected in 9-12hr embryos on northern blots and are detected at all subsequent stages tested. The two transcripts are present in roughly equal amounts until adulthood at which point the 3.0kb transcript predominates. n-syb transcripts are more abundant in adult head than body.

(DiAntonio et al., 1993)

n-syb transcripts are first detected in embryos at stage 13 and are restricted to the nervous system. They become quite abundant in both the central and peripheral nervous sytems. In adults, transcripts are detected in the cell bodies of the visual system, in the central brain and in the thoracic ganglia.

(DiAntonio et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

bouton

(Broadie et al., 1995)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

synapse of neuromuscular junction

(Deitcher et al., 1998)

neuromuscular junction of muscle cell of abdominal 1 ventral longitudinal muscle 3

(Deitcher et al., 1998)

neuromuscular junction of muscle cell of abdominal 1 ventral longitudinal muscle 4

(Deitcher et al., 1998)

larval intersegmental nerve

(Deitcher et al., 1998)

larval ventral nerve cord

(Deitcher et al., 1998)

larval segmental nerve

(Deitcher et al., 1998)

larval ventral nerve cord commissure

(Deitcher et al., 1998)

larval stage

neuromuscular junction

(Broadie et al., 1995)

synapse

(Broadie et al., 1995)

lamina

(Hiesinger et al., 2001)

photoreceptor neuron

(Hiesinger et al., 2001)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- third instar larval stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

n-syb protein staining is strong in the ventral nerve cord in embryos and is faint in axon commissures and segmental and intersegmental nerves. Staining is also apparent at the neuromuscular junction (NMJ). It is concentrated in the synaptic zones at the NMJ.

(Deitcher et al., 1998)

n-syb protein is largely restricted to the presynaptic boutons in larval neuromuscular junctions. Low levels of expression are seen in the motor axons.

(Broadie et al., 1995)

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in early endosome membrane

inferred from direct assay

(Haberman et al., 2012)

located_in plasma membrane

inferred from direct assay

(Estes et al., 2000)

colocalizes_with presynaptic active zone

inferred from direct assay

(Petersen and Stowers, 2011)

part_of SNARE complex

inferred from direct assay

(Yu et al., 2011, Wang and Chapman, 2010)

is_active_in synapse

inferred from direct assay

(Wang and Chapman, 2010)

is_active_in synaptic vesicle membrane

inferred from direct assay

(Haberman et al., 2012)

colocalizes_with synaptic vesicle

inferred from direct assay

(Petersen and Stowers, 2011)

located_in synaptic vesicle

inferred from direct assay

(Rolls et al., 2007)

located_in terminal bouton

inferred from direct assay

(Loveall and Deitcher, 2010)

Expression Deduced from Reporters

Reporter: P{GMR57C10-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

(Eschbach et al., 2021)

adult optic lobe neuron | subset

(Wu et al., 2016, Nern et al., 2015)

(Massey et al., 2019)

(Klapoetke et al., 2022)

Reporter: P{nSyb-GAL4.DBD::QF.AD}

Stage

Tissue/Position (including subcellular localization)

Reference

(Riabinina et al., 2015)

Reporter: P{nSyb-GAL4.P} M{nSyb-GAL4.P}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Bloomington Drosophila Stock Center, 2017, Riabinina and Potter, 2013.10.1)

(Riabinina et al., 2015)

(Tanaka and Clark, 2022)

Reporter: P{nSyb-GAL4.S}

Stage

Tissue/Position (including subcellular localization)

Reference

(Yu et al., 2010, Bushey et al., 2009)

late third instar larval stage

(Zhang et al., 2022)

Reporter: P{nSyb-GAL4.U}

Stage

Tissue/Position (including subcellular localization)

Reference

larval neuron | subset of larval brain

(Colombani et al., 2015)

Reporter: P{nSyb-GAL4.W}

Stage

Tissue/Position (including subcellular localization)

Reference

(McKinney et al., 2020)

Reporter: P{nSyb-GS.B}

Stage

Tissue/Position (including subcellular localization)

Reference

(Haynes et al., 2024)

(Jacobs and Sehgal, 2020, Bai et al., 2018)

Reporter: P{nSyb-lexA.DBD::G4.MD::QF.AD}

Stage

Tissue/Position (including subcellular localization)

Reference

(Riabinina et al., 2015)

Reporter: P{nSyb-lexA.DBD::QF.AD}

Stage

Tissue/Position (including subcellular localization)

Reference

(Riabinina et al., 2015)

wing nerve

(Szabó et al., 2023)

(Szabó et al., 2023)

Reporter: P{nSyb-QF2.P}

Stage

Tissue/Position (including subcellular localization)

Reference

(Riabinina et al., 2015)

Reporter: P{nSyb-QF2w.P}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

(Riabinina et al., 2015)

Reporter: PBac{R57C10-lexA::p65}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

(Liu et al., 2023)

Reporter: P{R57C10-P-lexA::p65}

Stage

Tissue/Position (including subcellular localization)

Reference

larval neuron

(Mohamed et al., 2023)

P-stage

(Bajar et al., 2022)

third instar larval stage

(Vagnoni and Bullock, 2016)

Reporter: PBac{nSyb-lexA::p65}

Stage

Tissue/Position (including subcellular localization)

Reference