FlyBase Gene Report: Dmel\Snap25

General Information

Symbol

Dmel\Snap25

Species

D. melanogaster

Name

Synaptosomal-associated protein 25kDa

Annotation Symbol

CG40452

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011288

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

Synaptosomal-associated protein 25kDa (Snap25) encodes a synaptic target SNARE protein and a component of SNARE protein complexes known to function in neurotransmitter release. It functions in synaptic transmission in larvae and is essential in adults. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

SNAP-25, Synapse protein 25

Key Links

Genomic Location

Cytogenetic map

80F9-80F9

Sequence location

3L:24,074,180..24,299,205 [+]

Recombination map

3-47

RefSeq locus

NT_037436 REGION:24074180..24299205

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Syt1; FB:FBgn0004242,FLYBASE:Syt4; FB:FBgn0028400

(Wang and Chapman, 2010)

enables SNAP receptor activity

inferred from direct assay

(Wang and Chapman, 2010)

enables SNARE binding

inferred from physical interaction with FLYBASE:nSyb; FB:FBgn0013342,FLYBASE:Syx1A; FB:FBgn0013343

(Wang and Chapman, 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables SNAP receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(GO Reference Genome Project, 2011-)

enables syntaxin binding

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(GO Reference Genome Project, 2011-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in regulation of short-term neuronal synaptic plasticity

inferred from direct assay

(Kawasaki and Ordway, 2009)

involved_in synaptic vesicle fusion to presynaptic active zone membrane

inferred from direct assay

(Yu et al., 2011)

involved_in synaptic vesicle priming

inferred from direct assay

(Kawasaki and Ordway, 2009)

involved_in synaptic vesicle transport

inferred from direct assay

(Kawasaki and Ordway, 2009)

involved_in vesicle fusion

inferred from direct assay

(Wang and Chapman, 2010)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in exocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(GO Reference Genome Project, 2011-)

involved_in neurotransmitter secretion

non-traceable author statement

(Lloyd et al., 2000)

involved_in synaptic vesicle docking

non-traceable author statement

(Littleton et al., 1999)

involved_in synaptic vesicle fusion to presynaptic active zone membrane

inferred from biological aspect of ancestor with PANTHER:PTN000447754

(GO Reference Genome Project, 2011-)

involved_in synaptic vesicle priming

inferred from biological aspect of ancestor with PANTHER:PTN000447754

(GO Reference Genome Project, 2011-)

involved_in vesicle-mediated transport

non-traceable author statement

(Lloyd et al., 2000)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of SNARE complex

inferred from direct assay

(Yu et al., 2011, Wang and Chapman, 2010)

located_in terminal bouton

inferred from direct assay

(Yu et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from sequence or structural similarity with UniProtKB:P60879

(Gene Ontology Curators, 2002-)

located_in membrane

inferred from sequence or structural similarity with UniProtKB:P60879

(Gene Ontology Curators, 2002-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000447752

(GO Reference Genome Project, 2011-)

part_of SNARE complex

inferred from sequence or structural similarity with UniProtKB:P60880-1

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000447753

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Qbc-SNAREs

Protein Family (UniProt)

Belongs to the SNAP-25 family. (P36975)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

Qbc-SNAREs -: Q-SNAREs are members of the SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein (SNAP) receptor) family that mediate vesicle-fusion. SNAREs are cytoplasmic, membrane-anchored proteins that contain a 60 amino acid SNARE domain. Three Q-SNAREs domains from the target membrane and one R-SNARE domain from the vesicle interact to form a four four-helix bundle that pulls the two membranes in close proximity and promotes fusion. After fusion, the complexes are dissembled by NSF (N-ethylmaleimide-sensitive factor) and SNAPs (Soluble NSF Attachment Proteins). Q-SNAREs are named from their contribution of a conserved glutamine (Q) residue to the helix bundle. Q-SNARE domains are designated a, b or c, depending on their position within the helix bundle. Qbc-SNAREs (or SNAPs (synaptosomal-associated proteins) possess a Qb and Qc domain, thus contribute two helices to the bundle. (Adapted from PMID:15893389 and FBrf0216182).

Protein Function (UniProtKB)

May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion (By similarity).

(UniProt, P36975)

Summary (Interactive Fly)

target-Snare (t-Snare) protein - one part of the four helix bundle of proteins referred to as SNAPs invoved in synaptic vesicle priming - required for vesicle docking at the presynapse - a part of the neuroexocytosis machinery

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Snap25 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P36975)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Snap25-RA

FBtr0111143

NM_001043176

2233

212

Snap25-RC

FBtr0304625

NM_001043177

2233

212

Additional Transcript Data and Comments

Reported size (kB)

2.3 (northern blot)

(Risinger et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Snap25-PA

FBpp0110435

23.7

212

4.24

FBpp0293167

23.7

212

4.20

NP_001036642

EAL24571

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

25 (kD observed)

(Risinger et al., 1997)

212 (aa); 23.7 (kD predicted)

(Risinger et al., 1993)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P36975

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Snap25 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Snap25_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.66

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

neuron

(Risinger et al., 1997)

larval ventral nerve cord

(Risinger et al., 1997)

adult stage

gnathal ganglion

(Risinger et al., 1993)

adult ventral nerve cord

• perikaryon

(Risinger et al., 1993)

abdominal neuromere

• perikaryon

(Risinger et al., 1993)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

(Risinger et al., 1993)

adult stage

(Risinger et al., 1993)

adult optic lobe

(Risinger et al., 1993)

adult brain

(Risinger et al., 1993)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

larval ventral nerve cord

(Risinger et al., 1997)

embryonic stage 16

chordotonal organ

(Risinger et al., 1997)

adult stage

neuron

• synapse

(Heifetz and Wolfner, 2004)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Snap25 protein is detected at high levels starting at embryonic stage 14. Most intense staining is seen in the dorsal extent of the ventral nerve cord, which is rich in synapses, and the esophageal ganglion. At stage 16, chordotonal organs of the PNS also stain for Snap25 protein.

(Risinger et al., 1997)

Marker for

synapse

(Heifetz and Wolfner, 2004)

Subcellular Localization

CV Term

Evidence

References

part_of SNARE complex

inferred from direct assay

(Yu et al., 2011, Wang and Chapman, 2010)

located_in terminal bouton

inferred from direct assay

(Yu et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Snap25 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Snap25

View exonic expression by tissue for Dmel\Snap25

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3355084/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011288

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011288

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011288

Flygut - An atlas of the Drosophila adult midgut

FBgn0011288

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 30 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Snap25

Transgenic constructs containing regulatory region of Snap25

Aberrations (Deficiencies and Duplications) ( 13 )

Inferred from experimentation ( 13 )

Gene not disrupted in

Df(3L)8A-80

(Deitcher, 2001.10.21)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Tub.PU

Snap25^HMS01367

lethal (with Df(3L)1-16)

lethal (with Df(3L)FX33)

Snap25^Z272

lethal (with Df(3L)FX53)

Snap25^Z272

lethal (with Df(3L)O-1)

Snap25^Z272

lethal | recessive

lethal - all die before end of P-stage | recessive

viable

Snap25^hs.PW

viable, with Scer\GAL4^Mef2.PR

Snap25^GD14547

viable, with Scer\GAL4^pnr-MD237

Snap25^GD14547

viable, with Scer\GAL4^Tub.PU

Snap25^JF02615

viable (with Df(3L)6B-29+Df(3R)6B-29)

Snap25^Z272

viable (with Df(3L)Δ1AK)

Snap25^Z272

viable (with Df(3R)XM3)

Snap25^Z272

Sterility

Allele

fertile, with Scer\GAL4^Tub.PU

Snap25^JF02615

Other Phenotypes

Allele

abnormal neuroanatomy

Snap25^ts

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.PG

Snap25^JF02615

abnormal neurophysiology

abnormal neurophysiology | heat sensitive

Snap25^ts

abnormal neurophysiology | temperature conditional (with Df(3L)1-16)

Snap25^ts

paralytic | heat sensitive

Snap25^ts

paralytic | heat sensitive (with Snap25^1-16-0)

Snap25^ts

paralytic | heat sensitive (with Snap25³)

Snap25^ts

paralytic | heat sensitive (with Snap25¹⁴)

Snap25^ts

paralytic | heat sensitive (with Snap25¹⁶)

Snap25^ts

paralytic | heat sensitive (with Snap25²²)

Snap25^ts

paralytic | heat sensitive (with Snap25²⁷)

Snap25^ts

paralytic | heat sensitive (with Snap25³³)

Snap25^ts

paralytic | heat sensitive (with Snap25³⁴)

Snap25^ts

paralytic | heat sensitive (with Snap25⁴²)

Snap25^ts

paralytic | heat sensitive (with Snap25⁴⁴)

Snap25^ts

paralytic | heat sensitive (with Snap25⁴⁶)

Snap25^ts

paralytic | heat sensitive (with Snap25⁴⁸)

Snap25^ts

paralytic | heat sensitive (with Snap25⁵²)

Snap25^ts

paralytic | heat sensitive (with Snap25⁵³)

Snap25^ts

paralytic | heat sensitive (with Snap25⁷⁰)

Snap25^ts

paralytic | heat sensitive (with Snap25⁸⁷)

Snap25^ts

paralytic | heat sensitive (with Snap25⁹³)

Snap25^ts

paralytic | heat sensitive (with Snap25¹¹⁴)

Snap25^ts

paralytic | heat sensitive (with Snap25¹²⁴)

Snap25^ts

paralytic | heat sensitive (with Snap25¹²⁵)

Snap25^ts

paralytic | heat sensitive (with Snap25¹²⁶)

Snap25^ts

paralytic | heat sensitive (with Snap25¹³²)

Snap25^ts

paralytic | heat sensitive (with Snap25¹³³)

Snap25^ts

paralytic | heat sensitive (with Snap25¹⁴⁷)

Snap25^ts

paralytic | heat sensitive (with Snap25¹⁵³)

Snap25^ts

paralytic | heat sensitive (with Snap25¹⁶⁴)

Snap25^ts

paralytic | heat sensitive (with Snap25⁶⁹⁵)

Snap25^ts

paralytic | recessive | heat sensitive

Snap25^ts

some die during pharate adult stage | recessive

Phenotype manifest in

Allele

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.PG

Snap25^JF02615

neuromuscular junction

neuromuscular junction | temperature conditional (with Df(3L)1-16)

Snap25^ts

t-bar

Snap25^ts

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (4)

Hsap\SNAP25

13 of 14

Yes

Hsap\SNAP23

11 of 14

Yes

Hsap\SNAP29

2 of 14

Hsap\SNAP47

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Snap25

11 of 14

Yes

Rnor\Snap23

10 of 14

Yes

Rnor\Snap29

2 of 14

Rnor\Snap47

1 of 14

Yes

Mus musculus (laboratory mouse) (4)

Mmus\Snap25

13 of 14

Yes

Mmus\Snap23

11 of 14

Yes

Mmus\Snap29

2 of 14

Mmus\Snap47

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (9)

Xtro\LOC100491227

7 of 13

Yes

Xtro\snap25

6 of 13

Xtro\snap23

5 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (6)

Drer\zgc:101731

14 of 14

Yes

Drer\snap25a

13 of 14

Drer\snap25b

13 of 14

Yes

Drer\snap23.1

12 of 14

Yes

Drer\snap23.2

6 of 14

Yes

Drer\snap29

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\ric-4

13 of 14

Yes

Cele\aex-4

4 of 14

Yes

Cele\snap-29

2 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP001394

11 of 12

Yes

Agam\AgaP_AGAP011135

2 of 12

Arabidopsis thaliana (thale-cress) (3)

Atha\SNAP29

10 of 13

Yes

Atha\SNAP30

10 of 13

Yes

Atha\SNAP33

10 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\SEC9

6 of 13

Yes

Scer\SPO20

4 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\sec9

6 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Snap25. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

Snap24

12 of 13

Snap29

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Snap25^JF02615

model of epilepsy

CEA

(Deneubourg et al., 2025)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SNAP25; synaptosome associated protein 25

model of congenital myasthenic syndrome 18

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Snap25^JF02615

exacerbates Parkinson's disease

modeled by Hsc70-5^KK100233

(Zhu et al., 2021)

model of epilepsy

is ameliorated by Epg5^KO

(Deneubourg et al., 2025)

ameliorates Vici syndrome

modeled by Epg5^KO

(Deneubourg et al., 2025)

Snap25^NIG.17676R

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-231.UAS}

(Onur et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SNAP25; synaptosome associated protein 25

13 of 14

600322

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 117; DEE117

congenital myasthenic syndrome 18

SNAP23; synaptosome associated protein 23

11 of 14

602534

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Snap25 - Bet1

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Membrin

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Nsf2

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Sec22

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Slh

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Snap24

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Snap29

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Syb

comigration in sds page, western blot, experimental knowledge based

(Guruharsha et al., 2011, Niemeyer and Schwarz, 2000)

Snap25 - Syt1

phenotype-based detection assay, inferred by author

(Wang and Chapman, 2010)

Snap25 - Syt4

phenotype-based detection assay, inferred by author

(Wang and Chapman, 2010)

Snap25 - Syx13

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Syx16

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Syx1A

anti bait coimmunoprecipitation, western blot, experimental knowledge based, pull down, comigration in sds page, phenotype-based detection assay, inferred by author

(Wang et al., 2014, Guruharsha et al., 2011, Yu et al., 2011, Wang and Chapman, 2010, Niemeyer and Schwarz, 2000)

Snap25 - Syx5

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Syx7

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Syx8

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Vamp7

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - Vha100-1

anti bait coimmunoprecipitation, western blot, bio-layer interferometry

(Wang et al., 2014)

Snap25 - Ykt6

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - cpx

anti tag coimmunoprecipitation, western blot

(Iyer et al., 2013)

Snap25 - koko

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - nSyb

phenotype-based detection assay, inferred by author, comigration in sds page, western blot

(Wang and Chapman, 2010, Niemeyer and Schwarz, 2000)

Snap25 - αSnap

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - γSnap1

experimental knowledge based

(Guruharsha et al., 2011)

Snap25 - γSnap2

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Snap25

suppressible

Snap24

(Vilinsky et al., 2002)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Hsc70-5

enhanceable

Snap25

(Zhu et al., 2021)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

78209

DroID - A comprehensive database of gene and protein interactions.

FBgn0011288

MIST (protein-protein) - An integrated Molecular Interaction Database

3355084

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47

Cytogenetic map

80F9-80F9

Sequence location

3L:24,074,180..24,299,205 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

80F9-80F9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

80-80

h47

(Drosophila Heterochromatin Genome Project, 2004)

FlyBase curator comment: FBrf0067338 stated that Snap25 mapped to clone DS04327 and thus cytological location 64E11--65B5, however subsequent analysis by Drosophila Heterochromatin Genome Project authoritatively determined that Snap25 lies in 3L heterochromatin.

(BDGP Project Members, 1994-1999)

FlyBase curator comment: FBrf0127207 stated that Snap25 mapped to 64E--65B, determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones, however subsequent analysis by Drosophila Heterochromatin Genome Project authoritatively determined that Snap25 lies in 3L heterochromatin.

(Lloyd et al., 2000)

Experimentally Determined Recombination Data

Location

3-47

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

51997

y¹ w^*; P{UAS-Snap25.L}9

81903

w^*; PBac{U6:3-Snap25.gRNA}VK00027

27306

y¹ v¹; P{TRiP.JF02615}attP2

80880

y¹, v¹; P{TKO.GS04784}attP40

81908

w^*; PBac{U6:3-Snap24-Snap25.gRNA}VK00027

81907

w^*; PBac{U6:3-Snap24-Snap25-Snap29.gRNA}VK00027

51617

P{GawB}elav^C155; P{UAS-Snap25.L}119/TM3, Sb¹

51624

Snap25⁴⁴ red¹ e¹/TM6B, Tb⁺

51623

Snap25¹²⁴ red¹ e¹/TM6B, Tb¹

34377

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01367}attP2

17676R-4

v330641

More stocks available...

Genomic Clones (58)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (74)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH28821

Other clones

RH47128

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011288

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011288

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Risinger et al., 1997)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2R+ : Stable cell lines co-expressing Snap25 (wildtype or P125A mutant) in combination with either Hip14, app, or Patsas.
(Porcellato et al., 2022)

Other Comments

Snap25 has an evolutionarily conserved and complex gene organisation. The onset of Snap25 expression correlates with a time in neuronal development when synapses begin to be formed and when other synapse-specific genes are switched on.

(Risinger et al., 1997)

Isolated from a Drosophila head cDNA library using a chicken SNAP-25 cDNA fragment (encoding the 5' half of the coding region) as a probe, under low stringency conditions.

(Risinger et al., 1993)

Snap25 has been cloned and sequenced, and its RNA expression pattern has been analysed.

(Risinger et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: Snap25 CG17884 CG17676

(Bayraktaroglu, 2000.4.12)

Source for merge of: CG17676 CG17884

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG17676 and CG17884 merged as CG40452 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG17676

CG17884

CG40452

(Meadows, 2018.10.31, Aradska et al., 2015, Meadows, 2014.12.12, White-Grindley et al., 2014, Khanna et al., 2010, Ni et al., 2010.12.1)

SNAP-25

(Sauvola and Littleton, 2021, Gundersen, 2020, Yoon et al., 2017, Harris and Littleton, 2015, Megighian et al., 2013, Kawasaki et al., 2011, Liu et al., 2011, Yu et al., 2011, Megighian et al., 2010, Rozas et al., 2010, Wang and Chapman, 2010, Kawasaki and Ordway, 2009, Kloepper et al., 2008, Lagow et al., 2007, Stowers and Isacoff, 2007, Heifetz and Wolfner, 2004, Bhattacharya et al., 2002, Verstreken and Bellen, 2002, Vilinsky et al., 2002, Vilinsky et al., 2002, Xue and Zhang, 2002, Deitcher, 2001.10.21, Littleton et al., 2001, Rao et al., 2001, Rivlin and Deitcher, 2001, Rivlin and Deitcher, 2001, Vilinsky and Deitcher, 2001, Littleton, 2000, Lloyd et al., 2000, Niemeyer and Schwarz, 2000, Nie et al., 1999, Niemeyer and Schwarz, 1999, Wu et al., 1999, Tolar and Pallanck, 1998, Wu et al., 1998, Risinger et al., 1997, Washbourne et al., 1997, Weimbs et al., 1997, Risinger, 1994.1.24, Risinger et al., 1993)

SNAP25

(Akai et al., 2025, Lee et al., 2025, Ataellahi et al., 2023, Quiñones-Frías and Littleton, 2021, Bulgari et al., 2019, Imler et al., 2019, Cho et al., 2014, Frank, 2014, Wang et al., 2014, Bykhovskaia et al., 2013, Iyer et al., 2013, Turner et al., 2013, Dickman et al., 2012, Kloepper et al., 2008, Ohyama et al., 2007, Drummond et al., 2001)

dSNAP-25

(Xu et al., 2014)

dSnap25

(Porcellato et al., 2022)

snap-25

(Syrzycka et al., 2019, Richmond and Broadie, 2002, Risinger, 1996.12.6, Risinger, 1996.12.6)

snap25

(Salzman et al., 2013)

snp25

(Tolar and Pallanck, 1998)

Name Synonyms

Synapse protein 25

(Chung et al., 2011, Khanna et al., 2010)

Synaptosomal-associated protein 25kDa

synaptosomal-associated protein 25

(Liu et al., 2011)

Secondary FlyBase IDs

FBan0040452
FBgn0039960
FBgn0039996

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 59 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3355084

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P36975

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P36975

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P36975

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3355084/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011288

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011288

FlyMine - An integrated database for Drosophila genomics

FBgn0011288

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG40452

MARRVEL_MODEL - MARRVEL (model organism gene)

3355084

Linkouts

BioGRID - A database of protein and genetic interactions.

78209

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011288

DroID - A comprehensive database of gene and protein interactions.

FBgn0011288

DRSC - Results frm RNAi screens

FBgn0011288

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Snap25_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011288

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011288

Flygut - An atlas of the Drosophila adult midgut

FBgn0011288

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0011288

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC010177

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

3355084

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (145)

The following external sites may use different assemblies or annotations than FlyBase.

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