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Citation
Wu, M.N., Fergestad, T., Lloyd, T.E., He, Y., Broadie, K., Bellen, H.J. (1999). Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo.  Neuron 23(3): 593--605.
FlyBase ID
FBrf0111523
Publication Type
Research paper
Abstract

Biochemical studies suggest that syntaxin 1A participates in multiple protein-protein interactions in the synaptic terminal, but the in vivo significance of these interactions is poorly understood. We used a targeted mutagenesis approach to eliminate specific syntaxin binding interactions and demonstrate that Drosophila syntaxin 1A plays multiple regulatory roles in neurotransmission in vivo. Syntaxin mutations that eliminate ROP/Munc-18 binding display increased neurotransmitter release, suggesting that ROP inhibits neurosecretion through its interaction with syntaxin. Syntaxin mutations that block Ca2+ channel binding also cause an increase in neurotransmitter release, suggesting that syntaxin normally functions in inhibiting Ca2+ channel opening. Additionally, we identify and characterize a syntaxin Ca2+ effector domain, which may spatially organize the Ca2+ channel, cysteine string protein, and synaptotagmin for effective excitation-secretion coupling in the presynaptic terminal.

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PubMed Central ID
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Erratum

Erratum.
Wu et al., 2000, Neuron 25(3): 1 after 728 [FBrf0128138]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference
    Alleles (8)
    Genes (7)
    Insertions (1)
    Transgenic Constructs (5)