FlyBase Allele Report: Dmel\Snap25[124]

General Information

Symbol

Dmel\Snap25¹²⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0141408

Feature type

allele

Associated gene

Dmel\Snap25

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Vilinsky et al., 2002)

amorphic allele - molecular evidence

(Vilinsky et al., 2002)

Mutagen

X ray

(Vilinsky et al., 2002)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Vilinsky et al., 2002)

lethal - all die before end of P-stage (with Df(3L)1-16)

(Vilinsky et al., 2002)

lethal - all die before end of P-stage | recessive

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25^ts)

(Vilinsky et al., 2002)

some die during pharate adult stage (with Df(3L)1-16)

(Vilinsky et al., 2002)

some die during pharate adult stage | recessive

(Vilinsky et al., 2002)

Phenotype Manifest In

neuromuscular junction

(Vilinsky et al., 2002)

Detailed Description

Statement

Reference

Homozygous larvae appear to behave normally; they can crawl and feed and withdraw upon prodding. Pupal development occurs normally and pharate adults have a beating heart, tanning of the cuticle and some weak pumping of the head capsule at the stage at which control animals being eclosing from the pupal case. If these animals are manually removed from the puparium they are capable of slight twitching movements of the forelegs and palps. They do not inflate their wings and survive for up to 18 hours if kept in a moist enclosed chamber at 22^o. The size of the excitatory junctional currents (EJCs) at the neuromuscular junction is not significantly different from controls in homozygous third instar larvae. The size and shape of miniature EJCs (mEJCs) is normal in the mutant larvae, but the frequency of mEJCs is about 50% of normal. Electroretinograms of mutant pharate adults show a complete lack of on- and off-transients.

(Vilinsky et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Snap25¹²⁴ has lethal | recessive | pharate adult stage phenotype, suppressible by Snap24^hs.PN

(Vilinsky et al., 2002)

Snap25¹²⁴ has abnormal neurophysiology phenotype, suppressible by Snap24^hs.PN

(Vilinsky et al., 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The presynaptic homeostatic plasticity that normally occurs after application of the postsynaptic glutamate receptor antagonist PhTx is blocked in Snap25¹²⁴/+ ; Df(2L)Exel6277/+ double heterozygous larvae. Synaptic homeostasis can be restored in the double heterozygotes through expression of Snapin^Scer\UAS.cDa in the motorneurons (under the control of Scer\GAL4^elav-C155).

(Dickman et al., 2012)

The electroretinogram defects of Snap25¹²⁴ pharate adults are rescued by expression Snap24^hs.PN.

(Vilinsky et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Snap25^ts

(Vilinsky et al., 2002)

Rescued by

Snap25¹²⁴/Df(3L)1-16 is rescued by Snap25^nSyb.PR

(Vilinsky et al., 2002)

Snap25¹²⁴ is rescued by Snap25^nSyb.PR

(Vilinsky et al., 2002)

Comments

The reduction in frequency of miniature excitatory junctional currents at the neuromuscular junction of Snap25¹²⁴ homozygous third instar larvae is rescued by Snap25^n-syb.PR. The electroretinogram defects of Snap25¹²⁴ pharate adults are rescued by Snap25^n-syb.PR.

(Vilinsky et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51623

Snap25¹²⁴ red¹ e¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Snap25¹²⁴

(Kawasaki and Ordway, 2009)

Snap25^mx124

(Dickman et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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