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General Information
Symbol
Dmel\Snap25ts
Species
D. melanogaster
Name
FlyBase ID
FBal0130594
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
SNAP-25ts
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Mutation at position 50 in the first amphipathic helix of Snap25.

    Amino acid replacement: G?E.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Snap25ts mutants display a short term depression phenotype in response to train stimulation at 33[o]C.

    At the restrictive temperature of 33[o]C, Snap25ts mutants exhibit a wild-type excitatory postsynaptic current (EPSC) in response to stimulus. However, subsequent stimulation produces a marked activity-dependent reduction in EPSC amplitude relative to wild-type.

    At the restrictive temperature of 33[o]C, Snap25ts mutants exhibit disrupted first component of recovery in PPD, which is greatly diminished in weight and exhibits a slower time constant. Moreover, both the weight and time constant of the slow recovery component are markedly increased.

    After 5Hz stimulation at 33[o]C, Snap25ts DLM neuromuscular synapses exhibit marked activity-dependent reduction in EPSC amplitude with respect to wild-type. Snap25ts synapses exhibit immediate enhancement of synaptic depression to a steady-state EPSC amplitude of 39.5 with respect to the first amplitude. Recovery in Snap25ts exhibits a similar time constant to the slow component in PP experiments.

    Snap25ts does not alter basic calcium-dependent properties of neurotransmitter release such as synaptic facilitation and calcium sensitivity.

    Snap25ts synapses stimulated at 33[o]C exhibit a wild-type first stimulation train but recover to only approximately 50% during the intertrain interval (compared to approximately 72% in wild-type).

    The mean evoked excitatory junctional potential (EJP) amplitude at the neuromuscular junction is increased to 35.5mV in Snap25ts/Df(3L)1-16 animals (compared to 24.7 mV in control animals) at 22o. The frequency of miniature EJPs is increased from 2.3Hz in control animals to 13.5Hz in Snap25ts/Df(3L)1-16 animals at 22o. At 37o, the mean EJP amplitude of Snap25ts/Df(3L)1-16 animals is reduced about 50% compared to controls.

    At 22oC evoked release of neurotransmitter is greatly increased as measured by excitatory junctional potentials. Quantal size is unaffected, though frequency of spontaneous release is 6 fold more frequent in mutants than wild type. There is no difference between mutants and wild type in the number of synaptic boutons. Flies can crawl, fly and mate. At 37oC release of neurotransmitter is reduced 60% compared with controls. Frequency of spontaneous release is not detectably different from controls. At 35oC and higher temperatures flies are paralyzed. Number, size and distribution of vesicles appears to be not significantly different from controls, though T-bars are more dense and filamentous than in wild type, particularly at 22oC. This may obscure docked vesicles close to the central beam of the T-bar.

    Mutants show an increase in the number of morphologically docked vesicles at the permissive temperature in the synapse as compared to control synapses.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT Enhanced by
    Statement
    Reference

    Snap25ts has abnormal neurophysiology | heat sensitive phenotype, non-enhanceable by shi1

    Suppressed by
    Statement
    Reference

    Snap25ts has paralytic | heat sensitive phenotype, suppressible | partially by Snap24hs.PN

    Snap25ts/Df(3L)1-16 has abnormal neurophysiology | temperature conditional phenotype, suppressible | partially by Snap24hs.PN

    NOT suppressed by
    Statement
    Reference

    Snap25ts has abnormal neurophysiology | heat sensitive phenotype, non-suppressible by shi1

    NOT Enhancer of
    Statement
    Reference

    Snap25ts is a non-enhancer of abnormal neurophysiology | heat sensitive phenotype of shi1

    NOT Suppressor of
    Statement
    Reference

    Snap25ts is a non-suppressor of abnormal neurophysiology | heat sensitive phenotype of shi1

    Phenotype Manifest In
    Suppressed by
    Statement
    Reference

    Snap25ts/Df(3L)1-16 has neuromuscular junction | temperature conditional phenotype, suppressible | partially by Snap24hs.PN

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    shi1;; Snap25ts double mutants display a short term depression phenotype in response to train stimulation at 33[o]C, as in both single mutants.

    Overexpression of Snap24hs.PN at 22o completely rescues the mean evoked excitatory junctional potential (EJP) amplitude at the neuromuscular junction in Snap25ts/Df(3L)1-16 animals and partially reduces the increased miniature EJP frequency of Snap25ts/Df(3L)1-16 animals. At 37oC, overexpression of Snap24hs.PN partially rescues the reduction in EJP amplitude seen in Snap25ts/Df(3L)1-16 animals. Expression of Snap24hs.PN in Snap25ts adults shifts the temperature of paralysis from 35o to 38o.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (6)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (8)