nSyb bearing v-SNARE vesicles contain a lipid-bound fluorescence resonance energy transfer donor-acceptor pair. When v-SNARE vesicles fuse to t-SNARE vesicles (bearing Syx1A and Snap25), the donor-acceptor pair is diluted, resulting in dequenching of the fluorescence signal.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.