FlyBase Allele Report: Dmel\Snap25[ts]

General Information

Symbol

Dmel\Snap25^ts

Species

D. melanogaster

Name

FlyBase ID

FBal0130594

Feature type

allele

Associated gene

Dmel\Snap25

Associated Insertion(s)

Carried in Construct

Also Known As

SNAP-25^ts

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutation at position 50 in the first amphipathic helix of Snap25.

(Rao et al., 2001)

Amino acid replacement: G?E.

(Rao et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Rivlin and Deitcher, 2001)

abnormal neurophysiology

(Rao et al., 2001)

abnormal neurophysiology | heat sensitive

(Kawasaki et al., 2011, Kawasaki and Ordway, 2009)

abnormal neurophysiology | temperature conditional (with Df(3L)1-16)

(Vilinsky et al., 2002)

paralytic | heat sensitive

(Rivlin and Deitcher, 2001)

paralytic | heat sensitive (with Snap25^1-16-0)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25³)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹⁴)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹⁶)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25²²)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25²⁷)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25³³)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25³⁴)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁴²)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁴⁴)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁴⁶)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁴⁸)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁵²)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁵³)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁷⁰)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁸⁷)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁹³)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹¹⁴)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹²⁴)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹²⁵)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹²⁶)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹³²)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹³³)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹⁴⁷)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹⁵³)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25¹⁶⁴)

(Vilinsky et al., 2002)

paralytic | heat sensitive (with Snap25⁶⁹⁵)

(Vilinsky et al., 2002)

paralytic | recessive | heat sensitive

(Rao et al., 2001)

Phenotype Manifest In

neuromuscular junction

(Rao et al., 2001)

neuromuscular junction | temperature conditional (with Df(3L)1-16)

(Vilinsky et al., 2002)

t-bar

(Rao et al., 2001)

Detailed Description

Statement

Reference

Snap25^ts mutants display a short term depression phenotype in response to train stimulation at 33[o]C.

(Kawasaki et al., 2011)

At the restrictive temperature of 33[o]C, Snap25^ts mutants exhibit a wild-type excitatory postsynaptic current (EPSC) in response to stimulus. However, subsequent stimulation produces a marked activity-dependent reduction in EPSC amplitude relative to wild-type.

At the restrictive temperature of 33[o]C, Snap25^ts mutants exhibit disrupted first component of recovery in PPD, which is greatly diminished in weight and exhibits a slower time constant. Moreover, both the weight and time constant of the slow recovery component are markedly increased.

After 5Hz stimulation at 33[o]C, Snap25^ts DLM neuromuscular synapses exhibit marked activity-dependent reduction in EPSC amplitude with respect to wild-type. Snap25^ts synapses exhibit immediate enhancement of synaptic depression to a steady-state EPSC amplitude of 39.5 with respect to the first amplitude. Recovery in Snap25^ts exhibits a similar time constant to the slow component in PP experiments.

Snap25^ts does not alter basic calcium-dependent properties of neurotransmitter release such as synaptic facilitation and calcium sensitivity.

Snap25^ts synapses stimulated at 33[o]C exhibit a wild-type first stimulation train but recover to only approximately 50% during the intertrain interval (compared to approximately 72% in wild-type).

(Kawasaki and Ordway, 2009)

The mean evoked excitatory junctional potential (EJP) amplitude at the neuromuscular junction is increased to 35.5mV in Snap25^ts/Df(3L)1-16 animals (compared to 24.7 mV in control animals) at 22^o. The frequency of miniature EJPs is increased from 2.3Hz in control animals to 13.5Hz in Snap25^ts/Df(3L)1-16 animals at 22^o. At 37^o, the mean EJP amplitude of Snap25^ts/Df(3L)1-16 animals is reduced about 50% compared to controls.

(Vilinsky et al., 2002)

At 22^oC evoked release of neurotransmitter is greatly increased as measured by excitatory junctional potentials. Quantal size is unaffected, though frequency of spontaneous release is 6 fold more frequent in mutants than wild type. There is no difference between mutants and wild type in the number of synaptic boutons. Flies can crawl, fly and mate. At 37^oC release of neurotransmitter is reduced 60% compared with controls. Frequency of spontaneous release is not detectably different from controls. At 35^oC and higher temperatures flies are paralyzed. Number, size and distribution of vesicles appears to be not significantly different from controls, though T-bars are more dense and filamentous than in wild type, particularly at 22^oC. This may obscure docked vesicles close to the central beam of the T-bar.

(Rao et al., 2001)

Mutants show an increase in the number of morphologically docked vesicles at the permissive temperature in the synapse as compared to control synapses.

(Rivlin and Deitcher, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Snap25^ts has abnormal neurophysiology | heat sensitive phenotype, non-enhanceable by shi¹

(Kawasaki et al., 2011)

Suppressed by

Statement

Reference

Snap25^ts/Df(3L)1-16 has abnormal neurophysiology | temperature conditional phenotype, suppressible | partially by Snap24^hs.PN

(Vilinsky et al., 2002)

Snap25^ts has paralytic | heat sensitive phenotype, suppressible by Snap24^hs.PN

(Vilinsky et al., 2002)

NOT suppressed by

Statement

Reference

Snap25^ts has abnormal neurophysiology | heat sensitive phenotype, non-suppressible by shi¹

(Kawasaki et al., 2011)

NOT Enhancer of

Statement

Reference

Snap25^ts is a non-enhancer of abnormal neurophysiology | heat sensitive phenotype of shi¹

(Kawasaki et al., 2011)

NOT Suppressor of

Statement

Reference

Snap25^ts is a non-suppressor of abnormal neurophysiology | heat sensitive phenotype of shi¹

(Kawasaki et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Snap25^ts/Df(3L)1-16 has neuromuscular junction | temperature conditional phenotype, suppressible | partially by Snap24^hs.PN

(Vilinsky et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

shi¹;; Snap25^ts double mutants display a short term depression phenotype in response to train stimulation at 33[o]C, as in both single mutants.

(Kawasaki et al., 2011)

Overexpression of Snap24^hs.PN at 22^o completely rescues the mean evoked excitatory junctional potential (EJP) amplitude at the neuromuscular junction in Snap25^ts/Df(3L)1-16 animals and partially reduces the increased miniature EJP frequency of Snap25^ts/Df(3L)1-16 animals. At 37^oC, overexpression of Snap24^hs.PN partially rescues the reduction in EJP amplitude seen in Snap25^ts/Df(3L)1-16 animals. Expression of Snap24^hs.PN in Snap25^ts adults shifts the temperature of paralysis from 35^o to 38^o.