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Citation
Williams, J.L., Shearin, H.K., Stowers, R.S. (2019). Conditional Synaptic Vesicle Markers for Drosophila.  G3 (Bethesda) 9(3): 737--748.
FlyBase ID
FBrf0241707
Publication Type
Research paper
Abstract

The release of neurotransmitters from synaptic vesicles (SVs) at pre-synaptic release sites is the principle means by which information transfer between neurons occurs. Knowledge of the location of SVs within a neuron can thus provide valuable clues about the location of neurotransmitter release within a neuron and the downstream neurons to which a given neuron is connected, important information for understanding how neural circuits generate behavior. Here the development and characterization of four conditional tagged SV markers for Drosophila melanogaster is presented. This characterization includes evaluation of conditionality, specificity for SV localization, and sensitivity of detection in diverse neuron subtypes. These four SV markers are genome-edited variants of the synaptic vesicle-specific protein Rab3. They depend on either the B2 or FLP recombinases for conditionality, and incorporate GFP or mCherry fluorescent proteins, or FLAG or HA epitope tags, for detection.

PubMed ID
PubMed Central ID
PMC6404611 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    G3 (Bethesda)
    Title
    G3 : genes - genomes - genetics
    ISBN/ISSN
    2160-1836
    Data From Reference
    Alleles (28)
    Genes (10)
    Natural transposons (2)
    Insertions (20)
    Experimental Tools (12)
    Transgenic Constructs (19)