FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4DBD.R29G11
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General Information
Symbol
Scer\GAL4DBD.R29G11
Species
S. cerevisiae
Name
FlyBase ID
FBal0295717
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R29G11-GAL4.DBD}
Also Known As
R29G11-DBD, R29G11DBD, R29G11-ZpGDBD, 29G11DBD, R29G11_ZpGdbd
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Chen et al., 2014)
Progenitor genotype
Carried in construct
P{R29G11-GAL4.DBD}
Cytology
Description

The R29G11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). An Hsp70 transcriptional terminator is present.

(Chen et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
GMR29G11
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      centrifugal neuron C3

      Comment: when combined with P{R26H02-p65.AD}

      when combined with P{R26H09-p65.AD}

      when combined with P{R35A03-p65.AD}

      (Takemura et al., 2017, Tuthill et al., 2013)
      lobula plate intrinsic neuron | subset

      Comment: when combined with P{R35A03-p65.AD}

      (Tuthill et al., 2013)
      lamina monopolar neuron L1 | subset

      Comment: when combined with P{R48A08-p65.AD}

      when combined with P{R75H09-p65.AD}

      (Tuthill et al., 2013)
      lamina monopolar neuron L2

      Comment: when combined with P{R48A08-p65.AD}

      when combined with P{R53G02-p65.AD} (combination referred to as 'L2')

      when combined with P{R53G02-p65.AD} (combination referred to as 'SS00801')

      when combined with P{R48H08-p65.AD}

      when combined with P{R53G02-p65.AD}

      when combined with P{R75H09-p65.AD}

      (Lillvis et al., 2022, Kind et al., 2021, Drews et al., 2020, von Reyn et al., 2017, Tuthill et al., 2013)
      alpha'/beta' Kenyon cell | intense

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Aso et al., 2014)
      alpha/beta core Kenyon cell | faint

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Aso et al., 2014)
      alpha/beta posterior Kenyon cell | intense

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Vogt et al., 2016, Aso et al., 2014)
      alpha/beta surface Kenyon cell | intense

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Vogt et al., 2016, Aso et al., 2014)
      gamma Kenyon cell | intense

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Aso et al., 2014)
      alpha'/beta' anterior-posterior Kenyon cell | intense

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Vogt et al., 2016)
      alpha'/beta' middle Kenyon cell | intense

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Vogt et al., 2016)
      alpha/beta core Kenyon cell

      Comment: when combined with P{R26E07-p65.AD} (combination referred to as 'MB417B')

      (Vogt et al., 2016)
      lamina monopolar neuron L1

      Comment: when combined with P{R48H08-p65.AD}

      (von Reyn et al., 2017)
      centrifugal neuron C3 | subset

      Comment: when combined with P{R26H02-p65.AD} (combination referred to as 'C3-Gal4')

      (Yuan et al., 2021)
      lamina monopolar neuron L2 | subset

      Comment: when combined with P{R53G02-p65.AD} (combination referred to as 'L2b-Gal4')

      (Yuan et al., 2021)
      Additional Information
      Statement
      Reference
       
      Marker for
      centrifugal neuron C3
      (Tuthill et al., 2013)
      lamina monopolar neuron L1
      (Tuthill et al., 2013)
      lamina monopolar neuron L2
      (Tuthill et al., 2013)
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R29G11 and Hsap\RELAAD.R48H08 in adults does not affect takeoff rates, compared to controls; spontaneous takeoffs are similar in duration to those of controls.

      Silencing (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R29G11 and Hsap\RELAAD.R48H08 in adults substantially and significantly reduces takeoff rates in response to looming stimuli, compared to controls; it also eliminates all aspects of giant fiber cell activity seen in controls in response to looming stimuli.

      (von Reyn et al., 2017)

      Blocking synaptic transmission (using shits1.IVS.p10.20xScer\UAS at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4DBD.R29G11 and Hsap\RELAAD.R26E07 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

      (Vogt et al., 2016)

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R29G11 and Hsap\RELAAD.R26E07 in adult males or females does not significantly affect the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

      (Sitaraman et al., 2015)

      Silencing of L1 + L2 lamina output neurons, using Hsap\RELAAD.R75H09.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, significantly affects fly responses to many different visual behavioural conditions.

      Silencing of L1 + L2 lamina output neurons, using Hsap\RELAAD.R48A08.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, significantly affects fly responses to many different visual behavioural conditions.

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R53G02.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, significantly affects fly responses to many different visual behavioural conditions.

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R53G02.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, impairs fly responses to monocular progressive but not regressive motion.

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R53G02.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, results in enhancement of reverse-optomotor inversion at high speeds.

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R53G02.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, compresses the height of the contrast sensitivity tuning function.

      Silencing of C3 lamina neurons, using Hsap\RELAAD.R35A03.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, abolishes steering responses to regressive motion stimuli moving at high speeds but does not affect responses to progressive motion or basic optomotor stimuli.

      Silencing of C3 lamina neurons, using Hsap\RELAAD.R26H02.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, abolishes steering responses to regressive motion stimuli moving at high speeds but does not affect responses to progressive motion or basic optomotor stimuli.

      Silencing of C3 lamina neurons, using Hsap\RELAAD.R35A03.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, results in enhancement of reverse-optomotor inversion at high speeds.

      Silencing of C3 lamina neurons, using Hsap\RELAAD.R26H02.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, results in enhancement of reverse-optomotor inversion at high speeds.

      Silencing of C3 lamina neurons, using Hsap\RELAAD.R35A03.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, affects the ability of flies to distinguish small contrast differences at low test speeds, while behaviour at higher test speeds remains normal.

      Silencing of C3 lamina neurons, using Hsap\RELAAD.R26H02.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, affects the ability of flies to distinguish small contrast differences at low test speeds, while behaviour at higher test speeds remains normal.

      (Tuthill et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (8)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Tuthill et al., 2013)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R29G11.T:Zzzz\Zip-
      Scer\GAL4DBD.R29G11.T:Zzzz\ZipRREEL
      Scer\GAL4DBD.R29G11
      Name Synonyms
      Secondary FlyBase IDs
        References (29)