FlyBase Allele Report: Scer\GAL4[DBD.R89B01]

General Information

Symbol

Scer\GAL4^DBD.R89B01

Species

S. cerevisiae

Name

FlyBase ID

FBal0304483

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R89B01-GAL4.DBD}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R89B01-GAL4.DBD}

Cytology

Description

The R89B01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR89B01

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

descending neuron of the gnathal ganglion DNg07

Comment: when combined with P{R42B02-p65.AD} (combination referred to as 'SS01074')

(Namiki et al., 2022, Namiki et al., 2018)

gamma Kenyon cell

Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB131B')

(Yamazaki et al., 2018)

gamma main Kenyon cell

Comment: when combined with P{R19B03-p65.AD} (combination referred to as 'MB108B')

when combined with P{R13F02-p65.AD} (combination referred to as 'MB131B')

(Yamazaki et al., 2018, Vogt et al., 2016, Aso et al., 2014)

gamma dorsal Kenyon cell | faint

Comment: when combined with P{R13F02-p65.AD} (combination referred to as 'MB131B')

(Vogt et al., 2016, Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | conditional, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R13F02

(Zhao et al., 2018)

abnormal locomotor behavior | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2015)

abnormal memory | adult stage | :heat sensitive | nutrition conditional, with Hsap\RELA^AD.R13F02, shi^{ts1.UAS.Tag:MYC}

(Yamazaki et al., 2018)

abnormal memory | adult stage | conditional, with Hsap\RELA^AD.R13F02, Npha\HR^{eNpHR2.0.UAS.cBa.YFP}

(Liu et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R13F02, shi^{ts1.UAS.Tag:MYC}

(Yamazaki et al., 2018)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R13F02, TrpA1^UAS.(B).cKa

(Yamazaki et al., 2018)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R19B03, shi^{ts1.UAS.Tag:MYC}

(Yamazaki et al., 2018)

abnormal sleep | adult stage | male limited | heat sensitive, with Hsap\RELA^AD.R19B03, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using shi^{ts1.UAS.Tag:MYC} at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02 in adults during retrieval, but not training or consolidation, significantly reduces 2 h aversive, or appetitive, olfactory memory, compared to controls; inhibition in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory in fed, but not starved, flies, compared to controls.

Inhibition (using shi^{ts1.UAS.Tag:MYC} at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R19B03 in adults during retrieval, but not training or consolidation, significantly reduces 2 h aversive or appetitive olfactory memory, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02 in adults during training, consolidation or retrieval significantly reduces 2 h aversive, or appetitive, olfactory memory, compared to controls; thermogenetic activation in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory in fed or starved flies, compared to controls.

(Yamazaki et al., 2018)

Adult males with inhibition (using Ctet\tetX^TNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02 have significantly weaker suppression of courtship behavior following training with mated females, compared to controls.

(Zhao et al., 2018)

Optogenetic stimulation (using Npha\HR^{e.UAS.cBa.YFP} with 593 nm (yellow) light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02 significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls.

(Liu et al., 2016)

Blocking synaptic transmission (using shi^{ts1.IVS.p10.20xScer\UAS} at 33[o]C) from neurons targeted by the split-GAL4 combination of Hsap\RELA^AD.R13F02 and Scer\GAL4^DBD.R89B01 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

(Vogt et al., 2016)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02 does not significantly affect the amount of sleep in adult males or females or affect locomotor activity of awake adult males, compared to controls; activation of neurons targeted by Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R19B03 significantly decreases the amount of sleep in males, but not females, and significantly decreases locomotor activity of awake adult males, compared to controls.

(Sitaraman et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

69303

w¹¹¹⁸; P{R89B01-GAL4.DBD}attP2

75840

w¹¹¹⁸; P{R42B02-p65.AD}attP40; P{R89B01-GAL4.DBD}attP2

68265

w¹¹¹⁸; P{R13F02-p65.AD}attP40/CyO; P{R89B01-GAL4.DBD}attP2

Notes on Origin

Discoverer

Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

(Aso et al., 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.R89B01.T:Zzzz\Zip-}

Scer\GAL4^DBD.R89B01

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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