FlyBase Allele Report: Hsap\RELA[AD.R72F11]

General Information

Symbol

Hsap\RELA^AD.R72F11

Species

H. sapiens

Name

FlyBase ID

FBal0319052

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R72F11-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Ohyama et al., 2015)

Progenitor genotype

Carried in construct

P{R72F11-p65.AD}

Cytology

Description

The GMR72F11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR72F11

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval Basin-4 neuron

Comment: when combined with P{R57F07-GAL4.DBD}

(Jovanic et al., 2016, Ohyama et al., 2015)

larval Basin-2 neuron

Comment: when combined with P{R38H09-GAL4.DBD}

(Jovanic et al., 2016)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage | chemical conditional, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R38H09

(Lopez-Bellido et al., 2019)

abnormal locomotor behavior | larval stage | chemical conditional, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R57F07

(Lopez-Bellido et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\tetX^TNT-LC.UAS) of neurons targeted by the split-GAL4 pairs of Scer\GAL4^DBD.R38H09 and Hsap\RELA^AD.R72F11, or Scer\GAL4^DBD.R57F07 and Hsap\RELA^AD.R72F11, significantly reduces larval escape behavior in response to HCl application, compared to controls.

(Lopez-Bellido et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions