Genetic reagents for making split-GAL4 lines in Drosophila.
The lines in the associated file were constructed for use with the split-GAL4 intersectional method (Luan et al. 2006, FBrf0193617) using the optimized vectors pBPZpGAL4DBDUw or pBPp65ADZpUw (Pfeiffer et al. 2010, FBrf0212052). In the split-GAL4 method, individual enhancers drive the expression of a non-functional half of GAL4 that contains either GAL4’s DNA-binding domain (DBD) or an activation domain (AD; in these constructs, the p65 activation domain is used) joined to a leucine-zipper dimerization domain. When these halves of GAL4 are present in the same cell, they combine to make a functional GAL4 protein that can bind to tandem arrays of its cognate UAS DNA sequence and activate transcription. Here we provide a set of transgenic such "hemidriver" lines expressing either the p65 AD or GAL4 DBD domain under the control of an enhancer chosen from either the collection generated at the Janelia Research Campus (Jenett et al. 2012, FBrf0219813) or at the Institute for Molecular Pathology (Tirian and Dickson 2017). BPp65ADZp and BPZpGDBD are hemidriver constructs that lack an enhancer fragment.
Please cite the following papers when using these lines in publications: For the Janelia enhancers: Dionne H, Hibbard KL, Cavallaro A, Kao J-C, Rubin GM. Genetic reagents for making split-GAL4 lines in Drosophila (https://www.biorxiv.org/content/early/2017/10/02/197509) For the VT enhancers: Tirian L, Dickson BJ. The VT GAL4, LexA, and split-GAL4 collection for targeted expression in the Drosophila nervous system (https://www.biorxiv.org/content/early/2017/10/05/198648). The HL9, Tdc2, ple (TH) and Trh hemidrivers are described in Aso et al. 2014 (FBrf0227179). These papers provide more details on the construction and use of the lines.