FB2026_02 , released June 18, 2026
Recombinant Construct: P{R12C11-p65.AD}
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General Information
Symbol
P{R12C11-p65.AD}
FlyBase ID
FBtp0099519
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\RELAAD.R12C11
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\RELAAD.R12C11
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR12C11
Encoded product / tool
p65(AD)::Zip+
Description

The R12C11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
larval Wave neuron

Comment: when combined with P{R20G03-GAL4.DBD} (combination referred to as 'MB120B')

(Takagi et al., 2017)
adult stage
mushroom body output neuron 1

Comment: when combined with P{R14C08-GAL4.DBD} (combination referred to as 'MB002B')

(Scaplen et al., 2021, Lewis et al., 2015, Aso et al., 2014)
mushroom body output neuron 3

Comment: when combined with P{R14C08-GAL4.DBD} (combination referred to as 'MB002B')

(Scaplen et al., 2021, Dolan et al., 2018, Lewis et al., 2015, Aso et al., 2014, Aso et al., 2014)
mushroom body output neuron 1 | faint

Comment: when combined with P{R14C08-GAL4.DBD} (combination referred to as 'MB002B')

(Aso et al., 2014)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Images
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

FlyBase curator comment: According to correspondence with the authors, some split combinations listed in the methods of the paper are incorrect; the correct combinations (MB002B=R12C11-AD+R14C08-DBD, MB316B=R58E02-AD+R93G08-DBD, MB056B=R76F05-AD+R80G12-DBD, MB047B=R58E02-AD+R93A09-DBD) are listed on FlyLight so these are the ones that have been associated with the paper.

(Lewis et al., 2015)
Stocks (3)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{R12C11-p65.AD}
(Takagi et al., 2017, FlyBase, 1992-)
R12C11
(Aso et al., 2014)
R12C11-ADp65
(Dolan et al., 2018)
R12C11-p65ADZp
(Lewis et al., 2015)
Secondary FlyBase IDs
    References (27)