FB2021_02, released April 13, 2021

Allele: Hsap\RELAAD.R18C11

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General Information
Symbol
Hsap\RELAAD.R18C11
Species
H. sapiens
Name
FlyBase ID
FBal0328444
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R18C11-p65.AD}
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Rubin Laboratory at Janelia Farm, 2017)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{R18C11-p65.AD}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The GMR18C11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

      (Rubin Laboratory at Janelia Farm, 2017)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      p65(AD)::Zip+
      Associated Sequence Features
      GMR18C11
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      antennal grooming descending neuron 1

      Comment: when combined with P{R71D01-GAL4.DBD}

      (Hampel et al., 2015)
      Additional Information
      Statement
      Reference

      In a split-GAL4 system, Hsap\RELAAD.R18C11 drives expression in antennal grooming descending neuron 1 when combined with Scer\GAL4DBD.R71D01. These neurons were seen in close proximity to Ecol\lexAGMR26B12 expressing antennal grooming brain interneuron 2 and Ecol\lexAGMR27H08 expressing zone C/E Johnston organ neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between C/E Johnston organ neurons and antennal grooming descending neuron 1 using these drivers.

      (Hampel et al., 2015)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1Scer\UAS.(B).cKa at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R71D01 and Hsap\RELAAD.R18C11 results in a significant increase of antennal grooming behavior compared to control flies; this phenotype persists following antennal amputation.

      (Hampel et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R18C11.T:Zzzz\Zip+
      Hsap\RELAAD.R18C11
      Name Synonyms
      Secondary FlyBase IDs
        References (3)