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General Information
Symbol
Hsap\RELAAD.R17A04
Species
H. sapiens
Name
FlyBase ID
FBal0304498
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
R17A04_p65ADZp
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The R17A04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
descending neuron of the posterior brain DNp28

Comment: when combined with P{R24A03-GAL4.DBD} (combination referred to as 'SS01056')

mushroom body calyx arborizing neuron 1 | intense

Comment: when combined with P{R65D07-GAL4.DBD} (combination referred to as 'MB380B')

lobula columnar neuron LC20

Comment: when combined with P{R35B06-GAL4.DBD} (combination referred to as 'SS00343')

adult ventral nerve cord | restricted

Comment: when combined with P{R35B06-GAL4.DBD} (combination referred to as 'SS00343')

mushroom body calyx arborizing neuron 1

Comment: when combined with P{R65D07-GAL4.DBD} (combination referred to as 'LH2449')

when combined with P{VT006903-GAL4.DBD} (combination referred to as 'LH1950')

when combined with P{VT020605-GAL4.DBD} (combination referred to as 'LH1995')

when combined with P{VT026759-GAL4.DBD} (combination referred to as 'LH1979')

when combined with P{VT041432-GAL4.DBD} (combination referred to as 'LH1900')

GABAergic neuron | subset

Comment: when combined with P{VT041432-GAL4.DBD} (combination referred to as 'LH1900')

Additional Information
Statement
Reference

In a split-GAL4 system, Hsap\RELAAD.R17A04 drives expression in lobula columnar neuron LC20 and a subset of neurons in the thoracico-abdominal ganglion when combined with Scer\GAL4DBD.R35B06.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R68A06 and Hsap\RELAAD.R17A04 in adults significantly increases takeoff rates, compared to controls; takeoffs are shorter in duration than spontaneous takeoffs in controls.

Silencing (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R68A06 and Hsap\RELAAD.R17A04 in adults mildly reduces overall takeoff rates in response to looming stimuli, and significantly and substantially reduces the proportion of short takeoffs, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (8)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\RELAAD.R17A04.T:Zzzz\Zip+
Hsap\RELAAD.R17A04
Name Synonyms
Secondary FlyBase IDs
    References (16)