Inhibition (using Hsap\KCNJ2^UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 in adults significantly increases food intake, but does not affect climbing rate, compared to controls.

Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08, significantly impairs aversive olfactory long term memory in adults, compared to controls.

Acute inhibition of endocytosis (using shi^1.UAS at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 during testing, 24 h after spaced, but not massed, training significantly impairs aversive olfactory long term memory in adults, compared to controls.

Inhibition of synaptic release (using shi^ts.UAS.cUa at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 significantly reduces carbon dioxide avoidance in starved or fed adults, compared to controls, but the effect is significantly smaller in fed flies.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 in adults results in a significant avoidance of the illuminated area, no avoidance is observed in controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 in adult males significantly reduces the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls; activation after sleep deprivation in adult males significantly reduces the amount of extra rebound sleep, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 in adult males does not significantly affect the amount of sleep, compared to controls; inhibition does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 in adult females in an area of an arena significantly increases avoidance of that area, compared to controls; simultaneous activation of these cells and neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R53C10 and Hsap\RELA^AD.R30E08 also increases avoidance, to a greater extent than either subset alone; there is no effect on angular speed or walking speed, compared to baseline, similar to controls, in either of these combinations.

Inhibition (using shi^{ts1.IVS.p10.20xUAS}, but not shi^1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 in adults during training, consolidation and testing substantially reduces associative aversive 2 h olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} or shi^1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction; inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) significantly reduces appetitive, but not aversive, 1 min associative visual memory, compared to controls, with no effect on sugar attraction; this inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition only during training and testing significantly reduces 24 h appetitive, but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa or TrpA1^{20xUAS.JFRC124} at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R15B01 and Hsap\RELA^AD.R14C08 in adults significantly reduces sleep, compared to controls, with no effect on social or locomotor behaviors.

Scer\GAL4^DBD.R15B01, Hsap\RELA^AD.R14C08, shi^{ts1.IVS.p10.20xUAS} is a suppressor | heat sensitive of abnormal sleep | adult stage | heat sensitive phenotype of Ecol\lexA^GMR35B12, TrpA1^{13xlexAop.JFRC26}

Scer\GAL4^DBD.R15B01, Hsap\RELA^AD.R14C08, shi^{ts1.IVS.p10.20xUAS} is a suppressor | heat sensitive of abnormal sleep | adult stage | heat sensitive phenotype of Ecol\lexA^GMR14H06, TrpA1^{13xlexAop.JFRC26}