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General Information
Symbol
Scer\GAL4DBD.R53C10
Species
S. cerevisiae
Name
FlyBase ID
FBal0304470
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The R53C10 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
mushroom body output neuron 6

Comment: when combined with P{R30E08-p65.AD} (combination referred to as 'MB434B')

mushroom body output neuron 5

Comment: when combined with P{R30E08-p65.AD} (combination referred to as 'MB434B')

adult wedge projection neuron 5

Comment: when combined with P{R10E07-p65.AD} (combination referred to as 'LH2448')

when combined with P{R41H07-p65.AD} (combination referred to as 'LH1337')

Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R53C10 and Hsap\RELAAD.R30E08 in adult males significantly reduces the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R53C10 and Hsap\RELAAD.R30E08 in adult females in an area of an arena significantly increases avoidance of that area, compared to controls; simultaneous activation of these cells and neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R15B01 and Hsap\RELAAD.R14C08 also increases avoidance, to a greater extent than either subset alone; there is no effect on angular speed or walking speed, compared to baseline, similar to controls, in either of these combinations.

Inhibition (using shits1.IVS.p10.20xUAS, but not shi1.UAS, at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R53C10 and Hsap\RELAAD.R30E08 in adults during training, consolidation and testing substantially reduces aversive, but not appetitive, 2 h associative olfactory memory, compared to controls; inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) during training, consolidation and testing significantly reduces appetitive, but not aversive, 1 min associative visual memory, compared to controls, with no effect on sugar attraction; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition only during training and testing significantly reduces 24 h appetitive, but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa or TrpA120xUAS.JFRC124 at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R53C10 and Hsap\RELAAD.R30E08 in adults significantly reduces sleep, compared to controls, with no effect on social or locomotor behaviors.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.R53C10.T:Zzzz\Zip-
Scer\GAL4DBD.R53C10
Name Synonyms
Secondary FlyBase IDs
    References (11)