FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{MB262B,MB083C,MB011B.x2}
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General Information
Symbol
P{MB262B,MB083C,MB011B.x2}
FlyBase ID
FBtp0172614
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\RELAAD.R14C08
Hsap\RELAAD.R52B07
Hsap\RELAAD.R52G04
Scer\GAL4DBD.R15B01
Scer\GAL4DBD.R52H01
Scer\GAL4DBD.R94B10
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

Carries two copies each of the same hemidriver pairs as in the MB262B, MB083C and MB011B split system combinations.

(Luo and Schnitzer, 2025.3.19)
Construct components
Component allele Scer\GAL4DBD.R15B01
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR15B01
Encoded product / tool
GAL4(DBD)::Zip-
Description

The R15B01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Component allele Scer\GAL4DBD.R94B10
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR94B10
Encoded product / tool
GAL4(DBD)::Zip-
Description

The R94B10 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Component allele Scer\GAL4DBD.R52H01
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR52H01
Encoded product / tool
GAL4(DBD)::Zip-
Description

The R52H01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Component allele Hsap\RELAAD.R14C08
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR14C08
Encoded product / tool
p65(AD)::Zip+
Description

The R14C08 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Component allele Hsap\RELAAD.R52B07
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR52B07
Encoded product / tool
p65(AD)::Zip+
Description

The R52B07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Rubin, 2017.6.14)
Component allele Hsap\RELAAD.R52G04
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR52G04
Encoded product / tool
p65(AD)::Zip+
Description

The R52G04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
first instar larval stage -- third instar larval stage
larval mushroom body output neuron d1

Comment: when combined with P{R11E07-p65.AD} (combination referred to as 'SS01705')

(Eschbach et al., 2021)
third instar larval stage
larval mushroom body dopaminergic neuron f1

Comment: when combined with P{R76F05-p65.AD} (combination referred to as 'MB054B')

(Eschbach et al., 2020)
larval mushroom body dopaminergic neuron g1

Comment: when combined with P{R76F05-p65.AD} (combination referred to as 'MB054B')

(Eschbach et al., 2020)
larval mushroom body output neuron d1

Comment: when combined with P{R11E07-p65.AD} (combination referred to as 'SS01705')

(Truman et al., 2023)
adult stage
mushroom body output neuron 3

Comment: when combined with P{R15B01-GAL4.DBD} (combination referred to as 'MB011B')

when combined with P{R14C08-p65.AD} (combination referred to as 'MB011B')

(Ichinose et al., 2021, Scaplen et al., 2021, Liu et al., 2016, Lewis et al., 2015, Aso et al., 2014, Aso et al., 2014)
mushroom body output neuron 1

Comment: when combined with P{R15B01-GAL4.DBD} (combination referred to as 'MB011B')

when combined with P{R14C08-p65.AD} (combination referred to as 'MB011B')

(Ichinose et al., 2021, Scaplen et al., 2021, Lewis et al., 2015, Aso et al., 2014, Aso et al., 2014)
mushroom body output neuron 4

Comment: when combined with P{R15B01-GAL4.DBD} (combination referred to as 'MB011B')

when combined with P{R14C08-p65.AD} (combination referred to as 'MB011B')

(Scaplen et al., 2021, Lewis et al., 2015, Aso et al., 2014, Aso et al., 2014)
mushroom body output neuron 8

Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB083C')

when combined with PBac{R20A02-p65.AD} (combination referred to as 'MB110C')

when combined with PBac{R52G04-p65.AD} (combination referred to as 'MB083C')

(Scaplen et al., 2021, Aso et al., 2014)
mushroom body output neuron 8 | intense

Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB083C')

when combined with PBac{R20A02-p65.AD} (combination referred to as 'MB110C')

when combined with PBac{R52G04-p65.AD} (combination referred to as 'MB083C')

(Aso et al., 2014)
mushroom body output neuron 9 | intense

Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB083C')

when combined with PBac{R20A02-p65.AD} (combination referred to as 'MB110C')

when combined with PBac{R52G04-p65.AD} (combination referred to as 'MB083C')

(Aso et al., 2014)
mushroom body output neuron 9

Comment: when combined with P{R94B10-GAL4.DBD} (combination referred to as 'MB083C')

when combined with PBac{R20A02-p65.AD} (combination referred to as 'MB110C')

when combined with PBac{R52G04-p65.AD} (combination referred to as 'MB083C')

(Ichinose et al., 2021, Scaplen et al., 2021)
mushroom body output neuron 11

Comment: when combined with P{R52H01-GAL4.DBD} (combination referred to as 'MB262B')

when combined with P{R52B07-p65.AD} (combination referred to as 'MB262B')

(Aso et al., 2014)
dopaminergic PAM neuron 13 | intense

Comment: when combined with P{R24E12-p65.AD} (combination referred to as 'MB025B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 14 | intense

Comment: when combined with P{R24E12-p65.AD} (combination referred to as 'MB025B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 13

Comment: when combined with P{R24E12-p65.AD} (combination referred to as 'MB025B')

(Dolan et al., 2018, Aso et al., 2014)
dopaminergic PAM neuron 14

Comment: when combined with P{R24E12-p65.AD} (combination referred to as 'MB025B')

(Dolan et al., 2018, Aso et al., 2014)
dopaminergic neuron

Comment: when combined with P{Ddc-GAL4.DBD}

(Gao et al., 2019)
proximal medullary amacrine neuron Pm4

Comment: when combined with P{R53C05-GAL4.DBD} (combination referred to as 'SS00317')

(Davis et al., 2020)
transmedullary neuron | subset

Comment: when combined with P{R53C05-GAL4.DBD} (combination referred to as 'SS00317')

(Davis et al., 2020)
mushroom body output neuron 7

Comment: when combined with P{R17C11-GAL4.DBD} (combination referred to as 'MB310C')

when combined with P{R60F01-GAL4.DBD} (combination referred to as 'MB311C')

when combined with P{R49F03-GAL4.DBD} (combination referred to as 'MB334C')

(Ichinose et al., 2021, Scaplen et al., 2021, Gao et al., 2019, Vogt et al., 2016, Ichinose et al., 2015, Aso et al., 2014, Aso et al., 2014)
mushroom body output neuron 7 | intense

Comment: when combined with P{R17C11-GAL4.DBD} (combination referred to as 'MB310C')

(Aso et al., 2014)
medulla columnar neuron VPN-MB2 | subset

Comment: when combined with P{R49F03-GAL4.DBD} (combination referred to as 'MB334C')

(Vogt et al., 2016)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
605725
w*; P{MB262B,MB083C,MB011B.x2}attP2/TM6B, Tb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{MB262B,MB083C,MB011B.x2}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)