Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04, significantly impairs aversive olfactory long term memory in adults, compared to controls.

Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R20A02, significantly impairs aversive olfactory long term memory in adults, compared to controls.

Acute inhibition of endocytosis (using shi^1.UAS at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04 during testing, 24 h after spaced, but not massed, training significantly impairs aversive olfactory long term memory in adults, compared to controls.

Acute inhibition of endocytosis (using shi^1.UAS at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R20A02 during testing, 24 h after spaced, but not massed, training significantly impairs aversive olfactory long term memory in adults, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04 in adult males does not significantly affect the amount of sleep, compared to controls; activation significantly increases locomotor activity of awake adult males, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04 in adult males does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04 in adult females in an area of an arena significantly increases attraction to that area, compared to controls; there is no effect on angular speed or walking speed, compared to baseline, similar to controls; simultaneous activation of these cells and neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R13F04 and Hsap\RELA^AD.R93D10, or Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 also increases attraction, to a similar extent, or greater extent, respectively, compared to either subset alone.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04 in adults during training, consolidation and testing does not substantially reduce aversive or attractive 2 h associative olfactory memory, or aversive or appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa or TrpA1^{20xUAS.JFRC124} at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R52G04 in adults significantly increases sleep, compared to controls, with no effect on social or locomotor behaviors.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R20A02 in adult females in an area of an arena significantly increases attraction to that area, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R94B10 and Hsap\RELA^AD.R20A02 in adults significantly increases sleep, compared to controls.