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General Information
Symbol
Scer\GAL4DBD.R19F09
Species
S. cerevisiae
Name
FlyBase ID
FBal0304437
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
R19F09
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The R19F09 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
mushroom body output neuron 13

Comment: when combined with P{R20G03-p65.AD} (combination referred to as 'MB018B')

when combined with PBac{R70B10-p65.AD} (combination referred to as 'MB051C')

when combined with PBac{R73H08-p65.AD} (combination referred to as 'MB090C')

when combined with P{R70B10-p65.AD} (combination referred to as 'MB051B')

mushroom body output neuron 12

Comment: when combined with P{R25D01-p65.AD} (combination referred to as 'MB077B')

when combined with PBac{R25D01-p65.AD} (combination referred to as 'MB077C')

when combined with PBac{R70B10-p65.AD} (combination referred to as 'MB051C')

Comment: when combined with P{R25D01-p65.AD} (combination referred to as 'MB077B')

mushroom body output neuron 13 | intense

Comment: when combined with P{R20G03-p65.AD} (combination referred to as 'MB018B')

mushroom body output neuron 12 | intense

Comment: when combined with P{R25D01-p65.AD} (combination referred to as 'MB077B')

when combined with P{R70B10-p65.AD} (combination referred to as 'MB051B')

adult wedge projection neuron 1

Comment: when combined with P{R25D01-p65.AD} (combination referred to as 'LH2447')

Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Hsap\KCNJ2UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 in adults does not affect food intake or climbing rate, compared to controls.

Inhibition of synaptic release (using shits1.IVS.p10.20xUAS at 32[o]C) in neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 during training and testing significantly impairs aversive odor-associated short term memory in adults, compared to controls; these flies also have significantly impaired aversive odor-associated short term memory when these neurons are inhibited (at 27[o]C) during testing only.

Inhibition (using shits1.UAS.Tag:MYC at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 in adults during training, consolidation or retrieval significantly reduces 2 h appetitive, but not aversive, olfactory memory, compared to controls.

Inhibition of the combined set of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Mef2.247 and Hsap\RELAAD.12xCRE and neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 in adults during training, consolidation or retrieval, significantly reduces 2 h appetitive, but not aversive, olfactory memory, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10, but not Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01, or Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R20G03, in adult males significantly increases the amount of sleep, compared to controls; activation of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10, but not Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01, or Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R20G03, significantly increases locomotor activity of awake adult males, compared to controls.

Inhibition (using shits1.IVS.p10.20xUAS at 31.5[o]C) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01, or Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R73H08, in non-sleep deprived adult males does not affect the amount of sleep, compared to controls; inhibition after sleep deprivation in adult males or females significantly reduces the amount of extra rebound sleep, compared to controls; inhibition does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R73H08 in adult females in an area of an arena significantly increases attraction to that area, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R73H08 in adults significantly increases sleep, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 in adult females in an area of an arena significantly increases attraction to that area, compared to controls; simultaneous activation of these cells and neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R13F04 and Hsap\RELAAD.R93D10 also increases attraction, to a greater extent than either subset alone; there is no effect on angular speed or walking speed, compared to baseline, similar to controls, in either of these combinations; simultaneous activation of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 and neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R94B10 and Hsap\RELAAD.R52G04, or the split-GAL4 pair of Scer\GAL4DBD.R11F03 and Hsap\RELAAD.R71D08, also increases attraction, to a greater extent, or a similar extent, respectively, compared to either subset alone.

Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 in adults during training, consolidation and testing does not substantially reduce aversive 2 h associative olfactory memory, but this (or inhibition using shi1.UAS) does reduce attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction. Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) does not significantly affect aversive or appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition only during training and testing significantly reduces 24 h appetitive, but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa or TrpA120xUAS.JFRC124 at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R25D01 in adults significantly increases sleep, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R20G03 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shits1.IVS.p10.20xUAS, but not shi1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R20G03 in adults during training, consolidation and testing substantially reduces aversive 2 h associative olfactory memory, compared to controls; inhibition (using shits1.IVS.p10.20xUAS or shi1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction. Inhibition of these neurons (using shits1.IVS.p10.20xUAS at 31-33[o]C) during training, consolidation and testing does not significantly affect appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition during training and testing significantly reduces 24 h appetitive (to the extent that this is aversive), but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R20G03 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10 in adults during training, consolidation and testing does not substantially reduce aversive 2 h associative olfactory memory, or aversive or appetitive 1 min associative visual memory, compared to controls; inhibition (using shits1.IVS.p10.20xUAS or shi1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction; inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls nad inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol.

Thermogenetic activation (using TrpA1UAS.(B).cKa or TrpA120xUAS.JFRC124 at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10 in adults significantly increases sleep, compared to controls, with no effect on social or locomotor behaviors.

External Data
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Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (5)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.R19F09.T:Zzzz\Zip-
Scer\GAL4DBD.R19F09
Name Synonyms
Secondary FlyBase IDs
    References (17)