Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03 in adult males does not significantly affect the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS}, but not shi^1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03 in adults during training, consolidation and testing substantially reduces aversive 2 h associative olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} or shi^1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction. Inhibition of these neurons (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) during training, consolidation and testing does not significantly affect appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition during training and testing significantly reduces 24 h appetitive (to the extent that this is aversive), but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R20G03 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.