Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R65B09, does not significantly affect aversive olfactory long term memory in adults, compared to wild-type controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pairs of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R71D08, or Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R65B09, in adult males does not significantly affect the amount of sleep, compared to controls; in the case of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R71D08, but not Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R65B09, activation significantly decreases locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R71D08 in adult females in an area of an arena significantly increases attraction to that area, compared to controls; there is no effect on angular speed or walking speed, compared to baseline, similar to controls; simultaneous activation of these cells and neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R19F09 and Hsap\RELA^AD.R25D01 also increases attraction, to a similar extent to either subset alone.

Inhibition (using shi^{ts1.IVS.p10.20xUAS}, but not shi^1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R71D08 in adults during training, consolidation and testing substantially reduces 2 h associative aversive or attractive olfactory memory, compared to controls; this inhibition also significantly reduces appetitive, but not aversive, 1 min associative visual memory, compared to controls, with no effect on sugar attraction; inhibition during testing alone significantly reduces aversive 2 h associative olfactory memory, compared to controls, but inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R71D08 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R65B09 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R65B09 in adults during training, consolidation and testing does not substantially reduce aversive 2 h associative olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} or shi^1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction; inhibition does not significantly affect appetitive 1 min associative visual memory and inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R11F03 and Hsap\RELA^AD.R65B09 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.