FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{R25D01-p65.AD}
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General Information
Symbol
PBac{R25D01-p65.AD}
FlyBase ID
FBtp0099535
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\RELAAD.R25D01
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\RELAAD.R25D01
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR25D01
Encoded product / tool
p65(AD)::Zip+
Description

The R25D01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
mushroom body output neuron 12

Comment: when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB077B')

when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB077C')

(Scaplen et al., 2021, Berry et al., 2018, Dolan et al., 2018, Yamazaki et al., 2018, Aso et al., 2014, Aso et al., 2014)

Comment: when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB077B')

(Yamazaki et al., 2018)
mushroom body output neuron 12 | intense

Comment: when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB077B')

(Aso et al., 2014)
adult wedge projection neuron 1

Comment: when combined with P{R19F09-GAL4.DBD} (combination referred to as 'LH2447')

(Dolan et al., 2019)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Images
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
601611
w1118; PBac{R25D01-p65.AD}VK00027
68284
w1118; P{R19F09-GAL4.DBD}attP2 PBac{R25D01-p65.AD}VK00027
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{R25D01-p65.AD}
(FlyBase, 1992-)
R25D01
(Aso et al., 2014)
Secondary FlyBase IDs
    References (11)