The R70B10 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB051B')
when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB051C')
Comment: when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB051B')
when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB051C')
Comment: when combined with P{R19F09-GAL4.DBD} (combination referred to as 'MB051B')
Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10 in adult males significantly increases the amount of sleep, compared to controls; activation significantly increases locomotor activity of awake adult males, compared to controls.
Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.
Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10 in adults during training, consolidation and testing does not substantially reduce aversive 2 h associative olfactory memory, or aversive or appetitive 1 min associative visual memory, compared to controls; inhibition (using shits1.IVS.p10.20xUAS or shi1.UAS, at 32[o]C) substantially reduces attractive 2 h associative olfactory memory, compared to controls, with no effect on naive sugar attraction; inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls nad inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol.
Thermogenetic activation (using TrpA1UAS.(B).cKa or TrpA120xUAS.JFRC124 at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19F09 and Hsap\RELAAD.R70B10 in adults significantly increases sleep, compared to controls, with no effect on social or locomotor behaviors.
Split-GAL4 transgene, constructed as described in FBrf0212052.