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General Information
Symbol
Rcom\RTA-cs2.UAS
Species
R. communis
Name
FlyBase ID
FBal0042323
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ricinCS, UAS-Ricin
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a cold-sensitive form of RT-A(cs2).

Allele components
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In the presence of Scer\GAL4ey-OK107 at low temperatures, deformed mushroom bodies (MBs), indicating leaky activity, are seen with P{UAS-RA.cs2}39, but not P{UAS-RA.cs2}CC. Two types of MB defects are noticed: axons crossing the midline and reduced number of vertical axons. In some severe cases, the alpha' lobe is missing. At 30 degrees C, P{UAS-RA.cs2}CC is as effective as P{UAS-RA.cs2}39, causing severe MB defects.

Expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4Cha.7.4 at 30[o]C impairs long term memory (1 day memory after spaced training is reduced compared to controls in an olfactory associative conditioning assay when the flies are shifted to 30[o]C immediately after training). This effect is still seen if the flies also carry Scer\GAL80Mef2.PT (which limits Scer\GAL4 expression to neurons outside of the mushroom body), but is not seen if the flies also carry Scer\GAL80cry.PS (which removes Scer\GAL4 expression from two dorsal-anterior-lateral neurons of the protocerebrum).

1 day memory after spaced training in an olfactory associative conditioning assay is intact in flies expressing activated Rcom\RAcs2.Scer\UAS during the 24 hour retention period (flies shifted to 30[o]C immediately after training and maintained at that temperature for 24 hours) when expression is driven by one of Scer\GAL4P0010, Scer\GAL4Feb170, Scer\GAL4Aph-4-c507, Scer\GAL4c42, Scer\GAL4c217, Scer\GAL4ey-OK107, Scer\GAL4G0050, Scer\GAL4E0973, Scer\GAL4c747, Scer\GAL4c739, Scer\GAL4c305a, Scer\GAL4c247, Scer\GAL4ple.PF, Scer\GAL4c316, Scer\GAL4GH146, Scer\GAL4Orco.PC, Scer\GAL4GMR.PF, Scer\GAL4repo.PU, Scer\GAL4VGlut.PD, Scer\GAL4Tdc2.PC, Scer\GAL4Gad1.3.098 or Scer\GAL4tim.PE.

Long term memory (1 day memory after spaced training in an olfactory associative conditioning assay) is impaired in flies expressing activated Rcom\RAcs2.Scer\UAS during the 24 hour retention period (flies shifted to 30[o]C immediately after training and maintained at that temperature for 24 hours) when expression is driven by one of Scer\GAL4cer-Mz1180, Scer\GAL4Ddc.PL, Scer\GAL4Hn.493, Scer\GAL4Hn.996, Scer\GAL4cry.PU or Scer\GAL4CaMKII.7.

Long term memory (1 day memory after spaced training in an olfactory associative conditioning assay) is impaired in flies expressing activated Rcom\RAcs2.Scer\UAS during the 24 hour retention period (flies shifted to 30[o]C immediately after training and maintained at that temperature for 24 hours) when expression is driven by Scer\GAL4per.PK. This defect is no longer seen if the Scer\GAL4per.PKflies also carry Scer\GAL80cry.PS (which removes Scer\GAL4 expression from two dorsal-anterior-lateral neurons of the protocerebrum).

1 day memory is disrupted after spaced training but not after massed training in an olfactory associative conditioning assay in flies in which Rcom\RAcs2.Scer\UAS is activated (by shifting the flies to 30[o]C) during the first 12 hours after training when expression of Rcom\RAcs2.Scer\UAS is driven by either Scer\GAL4E0946, Scer\GAL4G0338 or Scer\GAL4G0431.

Expression of Rcom\RAcs2.Scer\UAS under the control of either Scer\GAL4ap-md544, Scer\GAL4bbg-C96 or Scer\GAL4brk.PU from the early second instar until eclosion (animals initially raised at 18[o]C, and then switched to 29[o]C at the early second instar) results in a reduction in total wing area compared to control flies. The wings have normal shape, proportion and vein patterning.

Expression of Rcom\RAcs2.Scer\UAS under the control of either Scer\GAL4en-e16E or Scer\GAL4ci.PU from the early second instar until eclosion (animals initially raised at 18[o]C, and then switched to 29[o]C at the early second instar) results in a reduction in total wing area compared to control flies. A reduction in area of both the wing compartment expressing the Rcom\RAcs2.Scer\UAS transgene and the neighbouring, non-expressing wing compartment is seen in both cases.

BrdU incorporation is not significantly affected compared to controls in the posterior (Rcom\RAcs2.Scer\UAS expressing) compartment of wing discs expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C in third instar larvae. However, BrdU incorporation in the adjacent, non-expressing anterior compartment is strongly reduced.

BrdU incorporation is not significantly affected compared to controls in the dorsal (Rcom\RAcs2.Scer\UAS expressing) compartment of wing discs expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4ap-md544 at 30[o]C in third instar larvae. However, BrdU incorporation in the adjacent, non-expressing ventral compartment is strongly reduced.

A clear increase in the number of TUNEL-positive cells is seen in the territory of Rcom\RAcs2.Scer\UAS expression in wing discs expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C, as well as in adjacent cells that are not expressing Rcom\RAcs2.Scer\UAS.

Expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4tsh-md621 at 29oC during the second larval instar results in a reduction of proliferation in the genital disc.

Expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4how-24B at 29oC results in a severe loss of musculature in the embryo.

Flies expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4GMR.PF survive to adulthood at 180C. When adult flies are shifted to 290C prior to behavioural experiments, these flies still show optomotor responses.

Scer\GAL4C-734-mediated expression at 31oC for 8 hours causes an abrupt increase in the frequency of dead cells. Examination of Scer\GAL4 activity and subsequent Ecol\lacZ expression (Ecol\lacZScer\UAS.cBa) reveals that the dead cells do not migrate within the wing disc and are engulfed in place. Scer\GAL4en-e16E-mediated expression for 8-24 hours at 31oC causes the appearance of apoptotic cells in the posterior compartment of the wing disc. After a chase period of 8-24 hours at 17oC ectopic cell death is now observed in the anterior compartment, a temporal evolution is seen to the spreading effect. After induction of cell death there is a substantial increase in the rate of DNA synthesis. Induction of cell death after 24 hours of larval development does not affect the final size of the wings. Scer\GAL4zfh2-MS209-mediated expression causes cell death in the wing hinge and Scer\GAL4ap-md544-mediated expression causes cell death in the dorsal compartment of the wing disc.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
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Genetic Interactions
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Xenogenetic Interactions
Statement
Reference

Df(3L)H99/+ suppresses the increase in the number of TUNEL-positive cells seen in the wing discs in larvae expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. It also largely rescues the non-autonomous increase in BrdU incorporation which is seen in the non-expressing compartment in discs expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. Df(3L)H99/+ does not rescue the autonomous or the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.

DroncI29/+ suppresses the increase in the number of TUNEL-positive cells seen in the wing discs in larvae expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. It also largely rescues the non-autonomous increase in BrdU incorporation which is seen in the non-expressing compartment in discs expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. DroncI29/+ does not rescue the autonomous or the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.

Co-expression of BacA\p35Scer\UAS.cHa causes a clear autonomous reduction in the increase in the number of TUNEL-positive cells seen in the wing discs in larvae expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. It also causes a clear non-autonomous rescue of the increase in BrdU incorporation which is seen in the non-expressing compartment in discs expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C. Co-expression of BacA\p35Scer\UAS.cHa does not rescue the autonomous or the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.

Co-expression of Diap1EY00710 does not rescue the autonomous or the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.

The increase in the number of TUNEL-positive cells and the non-autonomous increase in BrdU incorporation seen in the wing discs in larvae expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C is largely suppressed by p53-ns.

The increase in the number of TUNEL-positive cells and the non-autonomous increase in BrdU incorporation seen in the wing discs in larvae expressing Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E at 30[o]C is largely suppressed by co-expression of either p53GD4483 or p53259H.GUS.

Co-expression of either p53Ct.GUS, p53GD4483 or p53259H.GUS does not rescue the autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E, but it does rescue the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.

Complementation and Rescue Data
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Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
RAcs2.UAS
Rcom\RAcs2.Scer\UAS
Rcom\RAcs2.UAS
Rcom\RTA-cs2.UAS
Rcom\RTcs2.UAS
Name Synonyms
Secondary FlyBase IDs
    References (13)