FB2026_02 , released June 18, 2026
Allele: Hsap\RELAAD.R11D03
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General Information
Symbol
Hsap\RELAAD.R11D03
Species
H. sapiens
Name
FlyBase ID
FBal0304346
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R11D03-p65.AD}
Also Known As
R11D03AD
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Tuthill et al., 2013)
Progenitor genotype
Carried in construct
P{R11D03-p65.AD}
Cytology
Description

The R11D03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Tuthill et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
GMR11D03
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      lamina wide-field 2 neuron | subset

      Comment: when combined with P{R19C10-GAL4.DBD}

      when combined with P{R19C10-GAL4.DBD} (combination referred to as 'Lawf2b-Gal4')

      when combined with P{R61H02-GAL4.DBD} (combination referred to as 'Lawf2a-Gal4')

      (Yuan et al., 2021, Tuthill et al., 2013)
      lamina wide-field 2 neuron

      Comment: when combined with P{R19C10-GAL4.DBD}

      when combined with P{R61H02-GAL4.DBD}

      (Tuthill et al., 2014, Tuthill et al., 2013)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R19C10 and Hsap\RELAAD.R11D03, or the split-GAL4 pair of Scer\GAL4DBD.R61H02 and Hsap\RELAAD.R11D03, in adult females increases flight turning responses to very low-frequency motion stimuli, without affecting responses to higher frequency motion, compared to controls; in the case of Scer\GAL4DBD.R19C10 and Hsap\RELAAD.R11D03 inhibited cells show normal overall morphology.

      (Tuthill et al., 2014)

      Silencing of Lawf2 lamina neurons, using Hsap\RELAAD.R11D03.T:Zzzz\Zip+, Scer\GAL4DBD.R19C10.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, results in enhancement of reverse-optomotor inversion at high speeds.

      Silencing of Lawf2 lamina neurons, using Hsap\RELAAD.R11D03.T:Zzzz\Zip+, Scer\GAL4DBD.R61H02.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, results in enhancement of reverse-optomotor inversion at high speeds.

      Silencing of Lawf2 lamina neurons, using Hsap\RELAAD.R11D03.T:Zzzz\Zip+, Scer\GAL4DBD.R19C10.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, affects the ability of flies to distinguish small contrast differences at low test speeds, while behaviour at higher test speeds remains normal.

      Silencing of Lawf2 lamina neurons, using Hsap\RELAAD.R11D03.T:Zzzz\Zip+, Scer\GAL4DBD.R61H02.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, affects the ability of flies to distinguish small contrast differences at low test speeds, while behaviour at higher test speeds remains normal.

      (Tuthill et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (4)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Tuthill et al., 2013)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R11D03.T:Zzzz\Zip+
      Hsap\RELAAD.R11D03
      Name Synonyms
      Secondary FlyBase IDs
        References (9)