FlyBase Allele Report: Hsap\RELA[AD.R11B11]

General Information

Symbol

Hsap\RELA^AD.R11B11

Species

H. sapiens

Name

FlyBase ID

FBal0328313

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R11B11-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R11B11-p65.AD}

Cytology

Description

The GMR11B11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR11B11

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

antennal grooming brain interneuron 1

Comment: when combined with P{R34C03-GAL4.DBD}

when combined with P{R71D01-GAL4.DBD}

(Hampel et al., 2015)

adult central complex

Comment: when combined with P{R71D01-GAL4.DBD}

(Hampel et al., 2015)

protocerebral bridge 1 glomerulus-fan-shaped body layer 2-lateral accessory lobe-crepine neuron

Comment: when combined with P{VT063731-GAL4.DBD} (combination referred to as 'SS53185')

(Wolff and Rubin, 2018)

adult neuron | subset of adult brain

Comment: when combined with P{R12D12-GAL4.DBD} (combination referred to as 'SS47731')

(Sterne et al., 2021)

adult roundup subesophageal neuron

Comment: when combined with P{R12D12-GAL4.DBD} (combination referred to as 'SS47731')

when combined with P{VT003236-GAL4.DBD} (combination referred to as 'SS47745')

(Sterne et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal grooming behavior | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R34C03

(Hampel et al., 2015)

abnormal grooming behavior | heat sensitive, with Scer\GAL4^DBD.R34C03, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

abnormal grooming behavior | heat sensitive, with Scer\GAL4^DBD.R71D01, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R34C03 and Hsap\RELA^AD.R11B11 or Scer\GAL4^DBD.R71D01 and Hsap\RELA^AD.R11B11 results in a significant increase of antennal grooming behavior compared to control flies; this phenotype persists following antennal amputation.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R34C03 and Hsap\RELA^AD.R11B11 induces antennal grooming, which persists for tens of seconds beyond red light cessation.

(Hampel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\RELA^AD.R11B11, Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R34C03 is a suppressor | partially of abnormal grooming behavior | heat sensitive phenotype of Ecol\lexA^GMR27H08, TrpA1^{13xlexAop.JFRC26}

(Hampel et al., 2015)

Hsap\RELA^AD.R11B11, Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R71D01 is a suppressor | partially of abnormal grooming behavior | heat sensitive phenotype of Ecol\lexA^GMR27H08, TrpA1^{13xlexAop.JFRC26}

(Hampel et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Inhibition of synaptic release (using Ctet\TeTxLC^TNT.Scer\UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R34C03 and Hsap\RELA^AD.R11B11 or Scer\GAL4^DBD.R71D01 and Hsap\RELA^AD.R11B11 significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA1^{13xlexAop.JFRC26} at 30-31[o]C) of Ecol\lexA^GMR27H08 neurons.