FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lexAGMR27H08
Open Close
General Information
Symbol
Ecol\lexAGMR27H08
Species
E. coli
Name
FlyBase ID
FBal0289266
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
P{GMR27H08-lexA}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2013.10.9)
Progenitor genotype
Carried in construct
P{GMR27H08-lexA}
Cytology
Description

A 2600 base pair fragment from the flanking non-coding or intronic region of mam is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)
Allele components
Component
Use(s)
Regulatory region(s)
GMR27H08
Encoded product / tool
lexA::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      zone C/E Johnston organ neuron | subset
      (Hampel et al., 2015)
      zone C Johnston organ neuron | subset
      (Hampel et al., 2015)
      zone E Johnston organ neuron | subset
      (Hampel et al., 2015)
      Additional Information
      Statement
      Reference

      EcollexAGMR27H08 neurons have distinct projections in the ventral adult subesophageal zone but show overlapping aborizations with zone C and E Johnston organ neurons in the antennal mechanosensory and motor center driven by ScerGAL4NP6303 or ScerGAL4NP6250, and close proximity to the following neuron projections; antennal grooming brain interneuron 1 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R34C03 and HsapRELAAD.R11B11, antennal grooming brain interneuron 2 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R26B12 and HsapRELAAD.R24C08, antennal grooming descending neuron 1 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R71D01 and HsapRELAAD.R18C11, and antennal grooming descending neuron 2 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R18C11 and HsapRELAAD.R76F12. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between C/E Johnston organ neurons and antennal grooming brain interneurons 1 & 2, antennal grooming descending neuron 1 and antennal grooming descending neuron 2 using the respective driver combinations above.

      (Hampel et al., 2015)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA113xlexAop.JFRC26 at 30-31[o]C) of Ecol\lexAGMR27H08 neurons results in a significant increase of antennal grooming behavior compared to control flies.

      (Hampel et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Inhibition of synaptic release (using Ctet\TeTxLCTNT.Scer\UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R60E02 and Hsap\RELAAD.R52F12 or Scer\GAL4DBD.R34C03 and Hsap\RELAAD.R11B11 or Scer\GAL4DBD.R71D01 and Hsap\RELAAD.R11B11 or Scer\GAL4DBD.R26B12 and Hsap\RELAAD.R24C08 or Scer\GAL4DBD.R26B12 and Hsap\RELAAD.R26A02 significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA113xlexAop.JFRC26 at 30-31[o]C) of Ecol\lexAGMR27H08 neurons.

      Inhibition of synaptic release (using Ctet\TeTxLCTNT.Scer\UAS) in Scer\GAL4GMR27H08 neurons significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA113xlexAop.JFRC26 at 30-31[o]C) of Ecol\lexAGMR27H08 neurons.

      Inhibition of synaptic release (using Ctet\TeTxLCTNT.Scer\UAS) in Scer\GAL4GMR18C11 neurons does not significantly suppress the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA113xlexAop.JFRC26 at 30-31[o]C) of Ecol\lexAGMR27H08 neurons.

      (Hampel et al., 2015)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

      (Pfeiffer et al., 2013.10.9)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\lexAGMR27H08
      Name Synonyms
      Secondary FlyBase IDs
        References (2)