FlyBase Allele Report: Hsap\RELA[AD.R24C08]

General Information

Symbol

Hsap\RELA^AD.R24C08

Species

H. sapiens

Name

FlyBase ID

FBal0328578

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R24C08-p65.AD}

Key Links

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Rubin Laboratory at Janelia Farm, 2017)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{R24C08-p65.AD}

Cytology

Nature of the lesion

Statement

Reference

The GMR24C08 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Product class / Tool use(s)

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Associated Sequence Features

GMR24C08

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | posterior | restricted

Comment: when combined with P{R26B12-GAL4.DBD}

(Hampel et al., 2015)

adult central complex

Comment: when combined with P{R26B12-GAL4.DBD}

(Hampel et al., 2015)

antennal grooming brain interneuron 2

Comment: when combined with P{R26B12-GAL4.DBD}

(Hampel et al., 2015)

adult lateral horn AV4b4 neuron

Comment: when combined with P{R20A03-GAL4.DBD} (combination referred to as 'LH2098')

(Dolan et al., 2019)

adult lateral horn AV6b3 neuron

Comment: when combined with P{R30E03-GAL4.DBD} (combination referred to as 'LH180')

(Dolan et al., 2019)

adult lateral horn PV2c1 neuron

Comment: when combined with P{R53C02-GAL4.DBD} (combination referred to as 'LH2111')

(Dolan et al., 2019)

Additional Information

Statement

Reference

In a split-GAL4 system, Hsap\RELA^AD.R24C08 drives expression in antennal grooming brain interneuron 2, the adult central complex and the posterior part of the brain when combined with Scer\GAL4^DBD.R26B12. Antennal grooming brain interneuron 2 were seen in close proximity to Ecol\lexA^GMR27H08 expressing zone C/E Johnston organ neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between C/E Johnston organ neurons and antennal grooming brain interneuron 2 using these drivers.

(Hampel et al., 2015)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal grooming behavior | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R26B12

(Hampel et al., 2015)

abnormal grooming behavior | heat sensitive, with Scer\GAL4^DBD.R26B12, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R26B12 and Hsap\RELA^AD.R24C08 results in a significant increase of antennal grooming behavior compared to control flies; there is a further significant increase in antennal grooming behavior following antennal amputation.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R26B12 and Hsap\RELA^AD.R24C08 induces antennal grooming, which persists for tens of seconds beyond red light cessation.

(Hampel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R24C08, Scer\GAL4^DBD.R26B12 is a suppressor | partially of abnormal grooming behavior | heat sensitive phenotype of Ecol\lexA^GMR27H08, TrpA1^{13xlexAop.JFRC26}

(Hampel et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Inhibition of synaptic release (using Ctet\TeTxLC^TNT.Scer\UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R26B12 and Hsap\RELA^AD.R24C08 significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA1^{13xlexAop.JFRC26} at 30-31[o]C) of Ecol\lexA^GMR27H08 neurons.

(Hampel et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

86714

w¹¹¹⁸; P{R24C08-p65.AD}attP40; P{R30E03-GAL4.DBD}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\RELA^{AD.R24C08.T:Zzzz\Zip+}

Hsap\RELA^AD.R24C08

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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