FB2026_03 , released September 17, 2026
Recombinant Construct: P{GMR27H08-lexA}
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General Information
Symbol
P{GMR27H08-lexA}
FlyBase ID
FBtp0088300
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lexAGMR27H08
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lexAGMR27H08
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR27H08
Encoded product / tool
lexA::p65
Description

A 2600 base pair fragment from the flanking non-coding or intronic region of mam is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
zone C/E Johnston organ neuron | subset
(Hampel et al., 2015)
zone C Johnston organ neuron | subset
(Hampel et al., 2015)
zone E Johnston organ neuron | subset
(Hampel et al., 2015)
Additional Information
Statement
Reference

EcollexAGMR27H08 neurons have distinct projections in the ventral adult subesophageal zone but show overlapping aborizations with zone C and E Johnston organ neurons in the antennal mechanosensory and motor center driven by ScerGAL4NP6303 or ScerGAL4NP6250, and close proximity to the following neuron projections; antennal grooming brain interneuron 1 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R34C03 and HsapRELAAD.R11B11, antennal grooming brain interneuron 2 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R26B12 and HsapRELAAD.R24C08, antennal grooming descending neuron 1 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R71D01 and HsapRELAAD.R18C11, and antennal grooming descending neuron 2 projections as labelled by the split-GAL4 pair ScerGAL4DBD.R18C11 and HsapRELAAD.R76F12. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between C/E Johnston organ neurons and antennal grooming brain interneurons 1 & 2, antennal grooming descending neuron 1 and antennal grooming descending neuron 2 using the respective driver combinations above.

(Hampel et al., 2015)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
52734
w1118; P{GMR27H08-lexA}attP40
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
27H08
(FlyBase, 1996-)
P{GMR27H08-lexA}
(FlyBase, 1996-)
R27H08-LexA
(Hampel et al., 2015)
aJO-LexA
(Hampel et al., 2015)
Secondary FlyBase IDs
    References (4)